BMRB: 7099

NMR Solution Structure of VP9 from White Spot Syndrome Virus

Authors

Liu, Y., Song, J.X., Hew, C.L.

Assembly

wsv230

Entity

1. wsv230 (polymer, Thiol state: all free), 85 monomers, 9492.592 Da Detail

GSHMATFQTD ADFLLVGDDT SRYEEVMKTF DTVEAVRKSD LDDRVYMVCL KQGSTFVLNG GIEELRLLTG DSTLEIQPMI VPTTE

Formula weight

9492.592 Da

Source organism

White spot syndrome virus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.5 %, Completeness: 76.6 %, Completeness (bb): 80.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	76.6 % (732 of 956)	82.8 % (405 of 489)	65.0 % (247 of 380)	92.0 % (80 of 87)
Backbone	80.8 % (409 of 506)	94.3 % (164 of 174)	66.3 % (165 of 249)	96.4 % (80 of 83)
Sidechain	75.6 % (400 of 529)	76.5 % (241 of 315)	75.7 % (159 of 210)	0.0 % (0 of 4)
Aromatic	15.0 % (9 of 60)	30.0 % (9 of 30)	0.0 % (0 of 30)
Methyl	85.8 % (91 of 106)	92.5 % (49 of 53)	79.2 % (42 of 53)

1. wsv230

GSHMATFQTD ADFLLVGDDT SRYEEVMKTF DTVEAVRKSD LDDRVYMVCL KQGSTFVLNG GIEELRLLTG DSTLEIQPMI VPTTE

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.72

#	Name	Isotope labeling	Type	Concentration
1	wsv230			3 mM
2	phosphate buffer			20 mM
3	H2O			90 %
4	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.32 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.32 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 10 models in PDB: 2GJI, Strand ID: A Detail

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Release date

2006-09-21

Citation

Identification of a novel nonstructural protein, VP9, from white spot syndrome virus: its structure reveals a ferredoxin fold with specific metal binding sites
Liu, Y., Wu, J.L., Song, J.X., Sivaraman, J., Hew, C.L.
J. Virol. (2006), 80, 10419-10427, PubMed 16956937 , DOI 10.1128/JVI.00698-06 , Abstract

Related entities 1. wsv230, : 1 : 1 : 1 : 7 entities Detail

Experiments performed 6 experiments Detail