High-resolution structural and thermodynamic analysis of extreme stabilization of human procarboxypeptidase by computational protein design
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 80.7 % (765 of 948) | 91.2 % (447 of 490) | 65.1 % (246 of 378) | 90.0 % (72 of 80) |
Backbone | 76.7 % (348 of 454) | 91.4 % (139 of 152) | 62.8 % (145 of 231) | 90.1 % (64 of 71) |
Sidechain | 85.4 % (486 of 569) | 91.1 % (308 of 338) | 76.6 % (170 of 222) | 88.9 % (8 of 9) |
Aromatic | 38.7 % (41 of 106) | 66.0 % (35 of 53) | 9.8 % (5 of 51) | 50.0 % (1 of 2) |
Methyl | 100.0 % (92 of 92) | 100.0 % (46 of 46) | 100.0 % (46 of 46) |
1. Designed Protein AYE
HHHHHHGSKT IFVIVPTNEE QVAFLEALAK QDELNFDWQN PPTEPGQPVV ILIPSDMVEW FLEMLKAKGI PFTVYVEEPressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Designed Protein AYE | 1 mM | ||
2 | phosphate buffer | 50 mM | ||
3 | KCl | 100 mM | ||
4 | H2O | 90 % | ||
5 | D2O | 10 % |