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BMRB: 7112

2HWT

1H, 13C, and 15N NMR Assignment of the Master-Rep Protein Nuclease Domain (2-95) from the Faba Bean Necrotic Yellows Virus

Authors

Vega-Rocha, S., Campos-Olivas, R.

Assembly

FBNYV M-Rep (2-95)

Entity

1. FBNYV M-Rep (2-95) (polymer, Thiol state: all free), 94 monomers, 10825.23 Da Detail

ARQVICWCFT LNNPLSPLSL HDSMKYLVYQ TEQGEAGNIH FQGYIEMKKR TSLAGMKKLI PGAHFEKRRG TQGEARAYSM KEDTRLEGPW EYGE

Formula weight

10825.23 Da

Source organism

Faba bean necrotic yellows virus

Exptl. method

NMR

Refine. method

30 conformers calculated using simulated annealing in torsion angle space with CYANA 2.1 (Models 2-31) and average structure after CYANA variable target function minimisation (Model 1)

Data set

assigned_chemical_shifts, coupling_constants, heteronucl_NOEs

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.7 %, Completeness (bb): 96.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.7 % (1076 of 1124)	95.8 % (567 of 592)	95.8 % (414 of 432)	95.0 % (95 of 100)
Backbone	96.8 % (538 of 556)	94.3 % (182 of 193)	99.3 % (271 of 273)	94.4 % (85 of 90)
Sidechain	95.4 % (623 of 653)	96.5 % (385 of 399)	93.4 % (228 of 244)	100.0 % (10 of 10)
Aromatic	84.9 % (90 of 106)	100.0 % (53 of 53)	68.6 % (35 of 51)	100.0 % (2 of 2)
Methyl	97.4 % (76 of 78)	94.9 % (37 of 39)	100.0 % (39 of 39)

1. FBNYV M-Rep (2-95)

ARQVICWCFT LNNPLSPLSL HDSMKYLVYQ TEQGEAGNIH FQGYIEMKKR TSLAGMKKLI PGAHFEKRRG TQGEARAYSM KEDTRLEGPW EYGE

Show sample condition

Sample #1

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	(2-95) M-Rep FBNYV	[U-10% 13C; U-15N]	0.5 mM
2	Sodium phosphate		20 mM
3	NaCl		600 mM
4	DTT		1 mM
5	D2O		8 %

Sample #2

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	(2-95) M-Rep FBNYV	[U-13C; U-15N]	0.5 mM
7	Sodium phosphate		20 mM
8	NaCl		600 mM
9	DTT		1 mM
10	D2O		8 %

Sample #3

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	2-95 Rep FBNYV	[U-13C; U-15N]	0.5 mM
12	Sodium phosphate		20 mM
13	NaCl		600 mM
14	DTT		1 mM
15	D2O		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.31 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.31 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.22 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 31 models in PDB: 2HWT, Strand ID: A Detail

Heteronucl. NOE

59 NOE values in 1 lists
Value type relative intensities, Field strength (¹H) 600 MHz, Temperature 293 (±0.5) K, pH 6.6 (±0.1) Detail

Coupling constant

70 J values in 1 lists
Temperature 293 (±0.5) K, pH 6.6 (±0.1) Detail

Release date

2006-11-12

Citation

1H, 13C, and 15N NMR assignment of the master Rep protein nuclease domain from the nanovirus FBNYV
Vega-Rocha, S., Gronenborn, A.M., Gronenborn, B., Campos-Olivas, R.
J. Biomol. NMR (2007), 38, 169-169, PubMed 17043745 , DOI 10.1007/s10858-006-9085-y , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 7218 released on 2006-10-29
Title 1H, 13C and 15N assignment of the Master-Rep protein nuclease domain from the Faba Bean Necrotic Yellows Virus

Related entities 1. FBNYV M-Rep (2-95), : 1 : 2 : 1 : 23 entities Detail

Experiments performed 17 experiments Detail

1 2D 1H15N_HSQC

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	(2-95) M-Rep FBNYV	[U-10% 13C; U-15N]	0.5 mM
2	Sodium phosphate		20 mM
3	NaCl		600 mM
4	DTT		1 mM
5	D2O		8 %

Sample #2

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	(2-95) M-Rep FBNYV	[U-13C; U-15N]	0.5 mM
7	Sodium phosphate		20 mM
8	NaCl		600 mM
9	DTT		1 mM
10	D2O		8 %

Sample #3

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	2-95 Rep FBNYV	[U-13C; U-15N]	0.5 mM
12	Sodium phosphate		20 mM
13	NaCl		600 mM
14	DTT		1 mM
15	D2O		100 %

2 2D 1H15N_HSQC long range

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	(2-95) M-Rep FBNYV	[U-10% 13C; U-15N]	0.5 mM
2	Sodium phosphate		20 mM
3	NaCl		600 mM
4	DTT		1 mM
5	D2O		8 %

Sample #2

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	(2-95) M-Rep FBNYV	[U-13C; U-15N]	0.5 mM
7	Sodium phosphate		20 mM
8	NaCl		600 mM
9	DTT		1 mM
10	D2O		8 %

Sample #3

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	2-95 Rep FBNYV	[U-13C; U-15N]	0.5 mM
12	Sodium phosphate		20 mM
13	NaCl		600 mM
14	DTT		1 mM
15	D2O		100 %

3 2D 1H13C_HSQC/HMQC

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	(2-95) M-Rep FBNYV	[U-10% 13C; U-15N]	0.5 mM
2	Sodium phosphate		20 mM
3	NaCl		600 mM
4	DTT		1 mM
5	D2O		8 %

Sample #2

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	(2-95) M-Rep FBNYV	[U-13C; U-15N]	0.5 mM
7	Sodium phosphate		20 mM
8	NaCl		600 mM
9	DTT		1 mM
10	D2O		8 %

Sample #3

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	2-95 Rep FBNYV	[U-13C; U-15N]	0.5 mM
12	Sodium phosphate		20 mM
13	NaCl		600 mM
14	DTT		1 mM
15	D2O		100 %

4 2D HNCOCG arom

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	(2-95) M-Rep FBNYV	[U-10% 13C; U-15N]	0.5 mM
2	Sodium phosphate		20 mM
3	NaCl		600 mM
4	DTT		1 mM
5	D2O		8 %

Sample #2

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	(2-95) M-Rep FBNYV	[U-13C; U-15N]	0.5 mM
7	Sodium phosphate		20 mM
8	NaCl		600 mM
9	DTT		1 mM
10	D2O		8 %

Sample #3

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	2-95 Rep FBNYV	[U-13C; U-15N]	0.5 mM
12	Sodium phosphate		20 mM
13	NaCl		600 mM
14	DTT		1 mM
15	D2O		100 %

5 2D HNCO arom

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	(2-95) M-Rep FBNYV	[U-10% 13C; U-15N]	0.5 mM
2	Sodium phosphate		20 mM
3	NaCl		600 mM
4	DTT		1 mM
5	D2O		8 %

Sample #2

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	(2-95) M-Rep FBNYV	[U-13C; U-15N]	0.5 mM
7	Sodium phosphate		20 mM
8	NaCl		600 mM
9	DTT		1 mM
10	D2O		8 %

Sample #3

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	2-95 Rep FBNYV	[U-13C; U-15N]	0.5 mM
12	Sodium phosphate		20 mM
13	NaCl		600 mM
14	DTT		1 mM
15	D2O		100 %

6 3D HNCO

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	(2-95) M-Rep FBNYV	[U-10% 13C; U-15N]	0.5 mM
2	Sodium phosphate		20 mM
3	NaCl		600 mM
4	DTT		1 mM
5	D2O		8 %

Sample #2

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	(2-95) M-Rep FBNYV	[U-13C; U-15N]	0.5 mM
7	Sodium phosphate		20 mM
8	NaCl		600 mM
9	DTT		1 mM
10	D2O		8 %

Sample #3

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	2-95 Rep FBNYV	[U-13C; U-15N]	0.5 mM
12	Sodium phosphate		20 mM
13	NaCl		600 mM
14	DTT		1 mM
15	D2O		100 %

7 3D HNCACB

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	(2-95) M-Rep FBNYV	[U-10% 13C; U-15N]	0.5 mM
2	Sodium phosphate		20 mM
3	NaCl		600 mM
4	DTT		1 mM
5	D2O		8 %

Sample #2

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	(2-95) M-Rep FBNYV	[U-13C; U-15N]	0.5 mM
7	Sodium phosphate		20 mM
8	NaCl		600 mM
9	DTT		1 mM
10	D2O		8 %

Sample #3

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	2-95 Rep FBNYV	[U-13C; U-15N]	0.5 mM
12	Sodium phosphate		20 mM
13	NaCl		600 mM
14	DTT		1 mM
15	D2O		100 %

8 3D CBCA (CO) NH

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	(2-95) M-Rep FBNYV	[U-10% 13C; U-15N]	0.5 mM
2	Sodium phosphate		20 mM
3	NaCl		600 mM
4	DTT		1 mM
5	D2O		8 %

Sample #2

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	(2-95) M-Rep FBNYV	[U-13C; U-15N]	0.5 mM
7	Sodium phosphate		20 mM
8	NaCl		600 mM
9	DTT		1 mM
10	D2O		8 %

Sample #3

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	2-95 Rep FBNYV	[U-13C; U-15N]	0.5 mM
12	Sodium phosphate		20 mM
13	NaCl		600 mM
14	DTT		1 mM
15	D2O		100 %

9 3D HNHA

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	(2-95) M-Rep FBNYV	[U-10% 13C; U-15N]	0.5 mM
2	Sodium phosphate		20 mM
3	NaCl		600 mM
4	DTT		1 mM
5	D2O		8 %

Sample #2

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	(2-95) M-Rep FBNYV	[U-13C; U-15N]	0.5 mM
7	Sodium phosphate		20 mM
8	NaCl		600 mM
9	DTT		1 mM
10	D2O		8 %

Sample #3

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	2-95 Rep FBNYV	[U-13C; U-15N]	0.5 mM
12	Sodium phosphate		20 mM
13	NaCl		600 mM
14	DTT		1 mM
15	D2O		100 %

10 3D HNHB

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	(2-95) M-Rep FBNYV	[U-10% 13C; U-15N]	0.5 mM
2	Sodium phosphate		20 mM
3	NaCl		600 mM
4	DTT		1 mM
5	D2O		8 %

Sample #2

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	(2-95) M-Rep FBNYV	[U-13C; U-15N]	0.5 mM
7	Sodium phosphate		20 mM
8	NaCl		600 mM
9	DTT		1 mM
10	D2O		8 %

Sample #3

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	2-95 Rep FBNYV	[U-13C; U-15N]	0.5 mM
12	Sodium phosphate		20 mM
13	NaCl		600 mM
14	DTT		1 mM
15	D2O		100 %

11 3D 15N edited TOCSY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	(2-95) M-Rep FBNYV	[U-10% 13C; U-15N]	0.5 mM
2	Sodium phosphate		20 mM
3	NaCl		600 mM
4	DTT		1 mM
5	D2O		8 %

Sample #2

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	(2-95) M-Rep FBNYV	[U-13C; U-15N]	0.5 mM
7	Sodium phosphate		20 mM
8	NaCl		600 mM
9	DTT		1 mM
10	D2O		8 %

Sample #3

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	2-95 Rep FBNYV	[U-13C; U-15N]	0.5 mM
12	Sodium phosphate		20 mM
13	NaCl		600 mM
14	DTT		1 mM
15	D2O		100 %

12 3D 15N edited NOESY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	(2-95) M-Rep FBNYV	[U-10% 13C; U-15N]	0.5 mM
2	Sodium phosphate		20 mM
3	NaCl		600 mM
4	DTT		1 mM
5	D2O		8 %

Sample #2

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	(2-95) M-Rep FBNYV	[U-13C; U-15N]	0.5 mM
7	Sodium phosphate		20 mM
8	NaCl		600 mM
9	DTT		1 mM
10	D2O		8 %

Sample #3

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	2-95 Rep FBNYV	[U-13C; U-15N]	0.5 mM
12	Sodium phosphate		20 mM
13	NaCl		600 mM
14	DTT		1 mM
15	D2O		100 %

13 3D HCACO

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	(2-95) M-Rep FBNYV	[U-10% 13C; U-15N]	0.5 mM
2	Sodium phosphate		20 mM
3	NaCl		600 mM
4	DTT		1 mM
5	D2O		8 %

Sample #2

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	(2-95) M-Rep FBNYV	[U-13C; U-15N]	0.5 mM
7	Sodium phosphate		20 mM
8	NaCl		600 mM
9	DTT		1 mM
10	D2O		8 %

Sample #3

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	2-95 Rep FBNYV	[U-13C; U-15N]	0.5 mM
12	Sodium phosphate		20 mM
13	NaCl		600 mM
14	DTT		1 mM
15	D2O		100 %

14 3D CC (CO) NH

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	(2-95) M-Rep FBNYV	[U-10% 13C; U-15N]	0.5 mM
2	Sodium phosphate		20 mM
3	NaCl		600 mM
4	DTT		1 mM
5	D2O		8 %

Sample #2

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	(2-95) M-Rep FBNYV	[U-13C; U-15N]	0.5 mM
7	Sodium phosphate		20 mM
8	NaCl		600 mM
9	DTT		1 mM
10	D2O		8 %

Sample #3

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	2-95 Rep FBNYV	[U-13C; U-15N]	0.5 mM
12	Sodium phosphate		20 mM
13	NaCl		600 mM
14	DTT		1 mM
15	D2O		100 %

15 HCCH TOCSY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	(2-95) M-Rep FBNYV	[U-10% 13C; U-15N]	0.5 mM
2	Sodium phosphate		20 mM
3	NaCl		600 mM
4	DTT		1 mM
5	D2O		8 %

Sample #2

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	(2-95) M-Rep FBNYV	[U-13C; U-15N]	0.5 mM
7	Sodium phosphate		20 mM
8	NaCl		600 mM
9	DTT		1 mM
10	D2O		8 %

Sample #3

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	2-95 Rep FBNYV	[U-13C; U-15N]	0.5 mM
12	Sodium phosphate		20 mM
13	NaCl		600 mM
14	DTT		1 mM
15	D2O		100 %

16 4D 15N, 13C NOESY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	(2-95) M-Rep FBNYV	[U-10% 13C; U-15N]	0.5 mM
2	Sodium phosphate		20 mM
3	NaCl		600 mM
4	DTT		1 mM
5	D2O		8 %

Sample #2

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	(2-95) M-Rep FBNYV	[U-13C; U-15N]	0.5 mM
7	Sodium phosphate		20 mM
8	NaCl		600 mM
9	DTT		1 mM
10	D2O		8 %

Sample #3

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	2-95 Rep FBNYV	[U-13C; U-15N]	0.5 mM
12	Sodium phosphate		20 mM
13	NaCl		600 mM
14	DTT		1 mM
15	D2O		100 %

17 4D 13C, 13C NOESY

Instrument

#1 Model Bruker Avance (600 MHz)

#2 Model Bruker Avance (700 MHz)

Sample #1

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	(2-95) M-Rep FBNYV	[U-10% 13C; U-15N]	0.5 mM
2	Sodium phosphate		20 mM
3	NaCl		600 mM
4	DTT		1 mM
5	D2O		8 %

Sample #2

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	(2-95) M-Rep FBNYV	[U-13C; U-15N]	0.5 mM
7	Sodium phosphate		20 mM
8	NaCl		600 mM
9	DTT		1 mM
10	D2O		8 %

Sample #3

Temperature 293 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	2-95 Rep FBNYV	[U-13C; U-15N]	0.5 mM
12	Sodium phosphate		20 mM
13	NaCl		600 mM
14	DTT		1 mM
15	D2O		100 %

nullKeywords M-Rep(2-95) of FBNYV

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BMRB: 7112

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Chemical shift reference

Chemical shift reference

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Chemical shift reference

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. FBNYV M-Rep (2-95), : 1 : 2 : 1 : 23 entities Detail

Experiments performed 17 experiments Detail

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