BMRB: 7120

The SAM domain of DLC1 defines a novel structure that interacts with elongation factor 1A1 at cortical actin and membrane ruffles

Authors

Yang, S.

Assembly

Rho-GTPase-activating protein 7

Entity

1. Rho-GTPase-activating protein 7 (polymer, Thiol state: all free), 76 monomers, 8952.386 Da Detail

MCRKKPDTMI LTQIEAKEAC DWLRATGFPQ YAQLYEDFLF PIDISLVKRE HDFLDRDAIE ALCRRLNTLN KCAVMK

Formula weight

8952.386 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.4 %, Completeness: 82.6 %, Completeness (bb): 81.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	82.6 % (766 of 927)	88.3 % (429 of 486)	72.1 % (261 of 362)	96.2 % (76 of 79)
Backbone	81.1 % (365 of 450)	96.7 % (145 of 150)	66.1 % (150 of 227)	95.9 % (70 of 73)
Sidechain	85.7 % (473 of 552)	84.5 % (284 of 336)	87.1 % (183 of 210)	100.0 % (6 of 6)
Aromatic	65.3 % (47 of 72)	66.7 % (24 of 36)	62.9 % (22 of 35)	100.0 % (1 of 1)
Methyl	100.0 % (86 of 86)	100.0 % (43 of 43)	100.0 % (43 of 43)

1. Rho-GTPase-activating protein 7

MCRKKPDTMI LTQIEAKEAC DWLRATGFPQ YAQLYEDFLF PIDISLVKRE HDFLDRDAIE ALCRRLNTLN KCAVMK

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	Rho-GTPase-activating protein 7	[U-15N; U-13C]		1 mM
2	sodium phosphate buffer			50 mM
3	DTT			3 mM
4	H2O			90 %
5	D2O			10 %

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LACS Plot; CA

Referencing offset: 0.18 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.18 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 10 models in PDB: 2GYT, Strand ID: A Detail

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Release date

2009-05-26

Citation

The SAM domain of DLC1 defines a novel structure that interacts with elongation factor 1A1 at cortical actin and membrane ruffles
Zhong, D., Yang, S., Zhang, J., Buschdorf, J.P., Yang, D., Low, B.C.

Related entities 1. Rho-GTPase-activating protein 7, : 1 : 1 : 22 entities Detail

Interaction partners 1. Rho-GTPase-activating protein 7, : 1 interactors Detail

Experiments performed 2 experiments Detail