BMRB: 7124

Solution Structure of MID1 B-box2 domain: A defining domain in TRIM/RBCC proteins reveals possible versatility in zinc-coordination

Authors

Matts, J.A.B., Short, K.M., Simmons, B.N., Singireddy, S., Zou, J., Cox, T.C., Massiah, M.A.

Assembly

B-box2 monomer

Entity

1. B-box2 (polymer, Thiol state: free and other bound), 47 monomers, 5381.154 Da Detail

SHIRGLMCLE HEDEKVNMYC VTDDQLICAL CKLVGRHRDH QVAALSE

2. ZN (non-polymer), 65.409 Da

Total weight

5446.563 Da

Max. entity weight

5381.154 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics, low target function

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 75.5 %, Completeness (bb): 78.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	75.5 % (400 of 530)	82.8 % (227 of 274)	61.2 % (126 of 206)	94.0 % (47 of 50)
Backbone	78.7 % (222 of 282)	90.6 % (87 of 96)	64.7 % (90 of 139)	95.7 % (45 of 47)
Sidechain	75.4 % (221 of 293)	78.7 % (140 of 178)	70.5 % (79 of 112)	66.7 % (2 of 3)
Aromatic	20.8 % (5 of 24)	41.7 % (5 of 12)	0.0 % (0 of 12)
Methyl	87.5 % (49 of 56)	89.3 % (25 of 28)	85.7 % (24 of 28)

1. B-box2

SHIRGLMCLE HEDEKVNMYC VTDDQLICAL CKLVGRHRDH QVAALSE

Show sample condition

Sample

Pressure 1 (±0) atm, Temperature 294 (±1) K, pH 7.5 (±.01)

#	Name	Isotope labeling	Type	Concentration
1	B-box2		protein	0.75 mM
2	ZN			0.0 ~ 0.0 mM
3	H2O			90 %
4	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.59 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.59 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 16 models in PDB: 2DQ5, Strand ID: A Detail

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Release date

2006-11-12

Citation

Solution structure of the RBCC/TRIM B-box1 domain of human MID1: B-box with a RING
Massiah, M.A., Simmons, B.N., Short, K.M., Cox, T.C.
J. Mol. Biol. (2006), 358, 532-545, PubMed 16529770 , DOI 10.1016/j.jmb.2006.02.009 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. B-box2, : 1 : 8 : 188 entities Detail

Interaction partners 1. B-box2, : 2 interactors Detail

Experiments performed 7 experiments Detail