BMRBj - BMREntryMaster

Found 1 Document (1.864 seconds)

BMRB: 7137

2H7T

NMR assignments of the C-terminal domain of insulin-like growth factor binding protein 2 (IGFBP-2)

Authors

KUANG, Z., YAO, S., KEIZER, D.W., WANG, C.C., BACH, L.A., FORBES, B.E., WALLACE, J.C., NORTON, R.S.

Assembly

C-TERMINAL DOMAIN OF INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 2

Entity

1. C-TERMINAL DOMAIN OF INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 2 (polymer, Thiol state: all disulfide bound), 107 monomers, 12219.75 Da Detail

GPPPARTPCQ QELDQVLERI STMRLPDERG PLEHLYSLHI PNCDKHGLYN LKQCKMSLNG QRGECWCVNP NTGKLIQGAP TIRGDPECHL FYNEQQEARG VHTQRMQ

Formula weight

12219.75 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS9:SG	1:CYS43:SG
2	disulfide	sing	1:CYS54:SG	1:CYS65:SG
3	disulfide	sing	1:CYS67:SG	1:CYS88:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.3 %, Completeness: 85.9 %, Completeness (bb): 92.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.9 % (1077 of 1254)	82.8 % (553 of 668)	91.1 % (430 of 472)	82.5 % (94 of 114)
Backbone	92.9 % (578 of 622)	93.9 % (200 of 213)	92.3 % (288 of 312)	92.8 % (90 of 97)
Sidechain	81.1 % (592 of 730)	77.6 % (353 of 455)	91.1 % (235 of 258)	23.5 % (4 of 17)
Aromatic	98.5 % (65 of 66)	97.0 % (32 of 33)	100.0 % (32 of 32)	100.0 % (1 of 1)
Methyl	95.5 % (84 of 88)	95.5 % (42 of 44)	95.5 % (42 of 44)

1. IGFBP-2

GPPPARTPCQ QELDQVLERI STMRLPDERG PLEHLYSLHI PNCDKHGLYN LKQCKMSLNG QRGECWCVNP NTGKLIQGAP TIRGDPECHL FYNEQQEARG VHTQRMQ

Show sample condition

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	IGFBP-2	[U-95% 15N]	0.8 mM
2	Na acetate		10 mM
3	azide		0.02 %
4	D2O		5 %

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	IGFBP-2	[U-95% 13C; U-90% 15N]	0.5 mM
6	Na acetate		10 mM
7	azide		0.02 %
8	D2O		5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl protons	null	indirect	0.2514495
¹H	TMS	methyl protons	internal	direct	1.0
¹⁵N	TMS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl protons	null	indirect	0.2514495
¹H	TMS	methyl protons	internal	direct	1.0
¹⁵N	TMS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl protons	null	indirect	0.2514495
¹H	TMS	methyl protons	internal	direct	1.0
¹⁵N	TMS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl protons	null	indirect	0.2514495
¹H	TMS	methyl protons	internal	direct	1.0
¹⁵N	TMS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2H7T, Strand ID: A Detail

Release date

2006-10-29

Citation

Structure, dynamics and heparin binding of the C-terminal domain of insulin-like growth factor-binding protein-2 (IGFBP-2)
KUANG, Z., YAO, S., KEIZER, D.W., WANG, C.C., BACH, L.A., FORBES, B.E., WALLACE, J.C., NORTON, R.S.
J. Mol. Biol. (2006), 364, 690-704, PubMed 17020769 , DOI 10.1016/j.jmb.2006.09.006 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P18065 released on 1990-11-01
Title IBP2_HUMAN Entity Insulin-like growth factor-binding protein 2

Related entities 1. C-TERMINAL DOMAIN OF INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 2, : 1 : 5 : 42 entities Detail

Interaction partners 1. C-TERMINAL DOMAIN OF INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 2, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 13 experiments Detail

1 1H15N_HSQC

Instrument

#1 Model Bruker AVANCE (500 MHz) Details CRYOPROBE

#2 Model Bruker DRX (600 MHz) Details TRIPLE-RESONANCE PROBE AND TRIPLE-AXIS GRADIENTS

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	IGFBP-2	[U-95% 15N]	0.8 mM
2	Na acetate		10 mM
3	azide		0.02 %
4	D2O		5 %

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	IGFBP-2	[U-95% 13C; U-90% 15N]	0.5 mM
6	Na acetate		10 mM
7	azide		0.02 %
8	D2O		5 %

2 1H13C_HSQC

Instrument

#1 Model Bruker AVANCE (500 MHz) Details CRYOPROBE

#2 Model Bruker DRX (600 MHz) Details TRIPLE-RESONANCE PROBE AND TRIPLE-AXIS GRADIENTS

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	IGFBP-2	[U-95% 15N]	0.8 mM
2	Na acetate		10 mM
3	azide		0.02 %
4	D2O		5 %

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	IGFBP-2	[U-95% 13C; U-90% 15N]	0.5 mM
6	Na acetate		10 mM
7	azide		0.02 %
8	D2O		5 %

3 1H15N_NOESY-HSQC

Instrument

#1 Model Bruker AVANCE (500 MHz) Details CRYOPROBE

#2 Model Bruker DRX (600 MHz) Details TRIPLE-RESONANCE PROBE AND TRIPLE-AXIS GRADIENTS

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	IGFBP-2	[U-95% 15N]	0.8 mM
2	Na acetate		10 mM
3	azide		0.02 %
4	D2O		5 %

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	IGFBP-2	[U-95% 13C; U-90% 15N]	0.5 mM
6	Na acetate		10 mM
7	azide		0.02 %
8	D2O		5 %

4 1H13C_NOESY-HSQC

Instrument

#1 Model Bruker AVANCE (500 MHz) Details CRYOPROBE

#2 Model Bruker DRX (600 MHz) Details TRIPLE-RESONANCE PROBE AND TRIPLE-AXIS GRADIENTS

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	IGFBP-2	[U-95% 15N]	0.8 mM
2	Na acetate		10 mM
3	azide		0.02 %
4	D2O		5 %

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	IGFBP-2	[U-95% 13C; U-90% 15N]	0.5 mM
6	Na acetate		10 mM
7	azide		0.02 %
8	D2O		5 %

5 HNCA

Instrument

#1 Model Bruker AVANCE (500 MHz) Details CRYOPROBE

#2 Model Bruker DRX (600 MHz) Details TRIPLE-RESONANCE PROBE AND TRIPLE-AXIS GRADIENTS

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	IGFBP-2	[U-95% 15N]	0.8 mM
2	Na acetate		10 mM
3	azide		0.02 %
4	D2O		5 %

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	IGFBP-2	[U-95% 13C; U-90% 15N]	0.5 mM
6	Na acetate		10 mM
7	azide		0.02 %
8	D2O		5 %

6 HN(CO)CA

Instrument

#1 Model Bruker AVANCE (500 MHz) Details CRYOPROBE

#2 Model Bruker DRX (600 MHz) Details TRIPLE-RESONANCE PROBE AND TRIPLE-AXIS GRADIENTS

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	IGFBP-2	[U-95% 15N]	0.8 mM
2	Na acetate		10 mM
3	azide		0.02 %
4	D2O		5 %

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	IGFBP-2	[U-95% 13C; U-90% 15N]	0.5 mM
6	Na acetate		10 mM
7	azide		0.02 %
8	D2O		5 %

7 HNCO

Instrument

#1 Model Bruker AVANCE (500 MHz) Details CRYOPROBE

#2 Model Bruker DRX (600 MHz) Details TRIPLE-RESONANCE PROBE AND TRIPLE-AXIS GRADIENTS

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	IGFBP-2	[U-95% 15N]	0.8 mM
2	Na acetate		10 mM
3	azide		0.02 %
4	D2O		5 %

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	IGFBP-2	[U-95% 13C; U-90% 15N]	0.5 mM
6	Na acetate		10 mM
7	azide		0.02 %
8	D2O		5 %

8 CBCA(CO)NH

Instrument

#1 Model Bruker AVANCE (500 MHz) Details CRYOPROBE

#2 Model Bruker DRX (600 MHz) Details TRIPLE-RESONANCE PROBE AND TRIPLE-AXIS GRADIENTS

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	IGFBP-2	[U-95% 15N]	0.8 mM
2	Na acetate		10 mM
3	azide		0.02 %
4	D2O		5 %

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	IGFBP-2	[U-95% 13C; U-90% 15N]	0.5 mM
6	Na acetate		10 mM
7	azide		0.02 %
8	D2O		5 %

9 HNCACB

Instrument

#1 Model Bruker AVANCE (500 MHz) Details CRYOPROBE

#2 Model Bruker DRX (600 MHz) Details TRIPLE-RESONANCE PROBE AND TRIPLE-AXIS GRADIENTS

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	IGFBP-2	[U-95% 15N]	0.8 mM
2	Na acetate		10 mM
3	azide		0.02 %
4	D2O		5 %

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	IGFBP-2	[U-95% 13C; U-90% 15N]	0.5 mM
6	Na acetate		10 mM
7	azide		0.02 %
8	D2O		5 %

10 HNHA

Instrument

#1 Model Bruker AVANCE (500 MHz) Details CRYOPROBE

#2 Model Bruker DRX (600 MHz) Details TRIPLE-RESONANCE PROBE AND TRIPLE-AXIS GRADIENTS

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	IGFBP-2	[U-95% 15N]	0.8 mM
2	Na acetate		10 mM
3	azide		0.02 %
4	D2O		5 %

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	IGFBP-2	[U-95% 13C; U-90% 15N]	0.5 mM
6	Na acetate		10 mM
7	azide		0.02 %
8	D2O		5 %

11 HBHA(CO)NH

Instrument

#1 Model Bruker AVANCE (500 MHz) Details CRYOPROBE

#2 Model Bruker DRX (600 MHz) Details TRIPLE-RESONANCE PROBE AND TRIPLE-AXIS GRADIENTS

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	IGFBP-2	[U-95% 15N]	0.8 mM
2	Na acetate		10 mM
3	azide		0.02 %
4	D2O		5 %

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	IGFBP-2	[U-95% 13C; U-90% 15N]	0.5 mM
6	Na acetate		10 mM
7	azide		0.02 %
8	D2O		5 %

12 HCCH-TOCSY

Instrument

#1 Model Bruker AVANCE (500 MHz) Details CRYOPROBE

#2 Model Bruker DRX (600 MHz) Details TRIPLE-RESONANCE PROBE AND TRIPLE-AXIS GRADIENTS

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	IGFBP-2	[U-95% 15N]	0.8 mM
2	Na acetate		10 mM
3	azide		0.02 %
4	D2O		5 %

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	IGFBP-2	[U-95% 13C; U-90% 15N]	0.5 mM
6	Na acetate		10 mM
7	azide		0.02 %
8	D2O		5 %

13 CNH-NOESY-HSQC

Instrument

#1 Model Bruker AVANCE (500 MHz) Details CRYOPROBE

#2 Model Bruker DRX (600 MHz) Details TRIPLE-RESONANCE PROBE AND TRIPLE-AXIS GRADIENTS

Sample #1

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	IGFBP-2	[U-95% 15N]	0.8 mM
2	Na acetate		10 mM
3	azide		0.02 %
4	D2O		5 %

Sample #2

Temperature 298 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Concentration
5	IGFBP-2	[U-95% 13C; U-90% 15N]	0.5 mM
6	Na acetate		10 mM
7	azide		0.02 %
8	D2O		5 %

null

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Found 1 Document (1.864 seconds)

BMRB: 7137

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. C-TERMINAL DOMAIN OF INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 2, : 1 : 5 : 42 entities Detail

Interaction partners 1. C-TERMINAL DOMAIN OF INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 2, : 2 interactors Detail

Experiments performed 13 experiments Detail

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