BMRB: 7147

The acute myeloid leukemia fusion protein AML1-ETO targets E-proteins via a PAH-like TAFH domain

Authors

Plevin, M.J., Zhang, J., Guo, C., Roeder, R.G., Ikura, M.

Assembly

Core-binding factor, ML1-ETO

Entity

1. Core-binding factor, ML1-ETO (polymer, Thiol state: not present), 105 monomers, 11872.60 Da Detail

GSARQLSKLK RFLTTLQQFG NDISPEIGER VRTLVLGLVN STLTIEEFHS KLQEATNFPL RPFVIPFLKA NLPLLQRELL HAARLAKQNP AQYLAQHEQL LLDAS

Formula weight

11872.6 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 82.7 %, Completeness (bb): 80.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	82.7 % (1058 of 1279)	93.1 % (623 of 669)	68.2 % (339 of 497)	85.0 % (96 of 113)
Backbone	80.6 % (498 of 618)	96.6 % (201 of 208)	65.0 % (202 of 311)	96.0 % (95 of 99)
Sidechain	86.0 % (655 of 762)	91.5 % (422 of 461)	80.8 % (232 of 287)	7.1 % (1 of 14)
Aromatic	37.5 % (30 of 80)	75.0 % (30 of 40)	0.0 % (0 of 40)
Methyl	98.6 % (144 of 146)	100.0 % (73 of 73)	97.3 % (71 of 73)

1. Core-binding factor, ML1-ETO

GSARQLSKLK RFLTTLQQFG NDISPEIGER VRTLVLGLVN STLTIEEFHS KLQEATNFPL RPFVIPFLKA NLPLLQRELL HAARLAKQNP AQYLAQHEQL LLDAS

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Core-binding factor, ML1-ETO	[U-15N; U-13C]		0.7 mM
2	sodium phosphate			20 mM
3	NaCl			50 mM
4	PMSF			1 mM
5	AEBSF			1 mM
6	sodium azide			0.25 mM
7	H2O			90 %
8	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.49 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.101329

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LACS Plot; CB

Referencing offset: -0.49 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.101329

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.101329

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Protein Blocks Logo

Calculated from 20 models in PDB: 2H7B, Strand ID: A Detail

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Release date

2006-10-29

Citation

The acute myeloid leukemia fusion protein AML1-ETO targets E proteins via a paired amphipathic helix-like TBP-associated factor homology domain
Plevin, M.J., Zhang, J., Guo, C., Roeder, R.G., Ikura, M.
Proc. Natl. Acad. Sci. U. S. A. (2006), 103, 10242-10247, PubMed 16803958 , DOI 10.1073/pnas.0603463103 , Abstract

Related entities 1. Core-binding factor, ML1-ETO, : 1 : 6 : 19 entities Detail

Interaction partners 1. Core-binding factor, ML1-ETO, : 2 interactors Detail

Experiments performed 2 experiments Detail