BMRB: 7151

Backbone 1H, 13C, and 15N Chemical Shift Assignments for DLC2-SAM

Authors

JIN, D., LI, H., FUNG, K., CHUNG, S., CHING, Y., NG, I., SZE, K., KO, B., SUN, H.

Assembly

DLC2-SAM monomer

Entity

1. DLC2-SAM monomer (polymer, Thiol state: all free), 81 monomers, 9323.442 Da Detail

MHHHHHHSSG LVPRGSQEIE AKEACDWLRA AGFPQYAQLY EDSQFPINIV AVKNDHDFLE KDLVEPLCRR LNTLNKCASM K

Formula weight

9323.442 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing, molecular dynamics, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.6 %, Completeness: 88.7 %, Completeness (bb): 91.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.7 % (845 of 953)	90.4 % (450 of 498)	85.6 % (316 of 369)	91.9 % (79 of 86)
Backbone	91.0 % (435 of 478)	91.9 % (148 of 161)	90.4 % (217 of 240)	90.9 % (70 of 77)
Sidechain	87.2 % (482 of 553)	89.6 % (302 of 337)	82.6 % (171 of 207)	100.0 % (9 of 9)
Aromatic	48.8 % (42 of 86)	60.5 % (26 of 43)	35.7 % (15 of 42)	100.0 % (1 of 1)
Methyl	100.0 % (76 of 76)	100.0 % (38 of 38)	100.0 % (38 of 38)

1. SAM domain of Deleted in Liver Cancer 2

MHHHHHHSSG LVPRGSQEIE AKEACDWLRA AGFPQYAQLY EDSQFPINIV AVKNDHDFLE KDLVEPLCRR LNTLNKCASM K

Show sample condition

Sample

Temperature 293 (±0.2) K

#	Name	Isotope labeling	Type	Concentration
1	SAM domain of Deleted in Liver Cancer 2	[U- 13C; U-15N]		1.3 mM
2	phosphate buffer			20 mM
3	DTT			4 mM
4	NaN3			0.05 %

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LACS Plot; CA

Referencing offset: 0.14 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.14 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.19 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.46 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2H80, Strand ID: A Detail

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Release date

2007-04-17

Citation

Solution structures, dynamics, and lipid-binding of the sterile alpha-motif domain of the deleted in liver cancer 2
LI, H., FUNG, K., JIN, D., CHUNG, S., CHING, Y., NG, I., SZE, K., KO, B., SUN, H.
Proteins (2007), 67, 1154-1166, PubMed 17380510 , DOI 10.1002/prot.21361 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. DLC2-SAM monomer, : 1 : 1 : 10 entities Detail

Interaction partners 1. DLC2-SAM monomer, : 11 interactors Detail

Experiments performed 10 experiments Detail