BMRBj - BMREntryMaster

Found 1 Document (2.77 seconds)

BMRB: 7158

2GQE

Molecular characterization of the Ran binding zinc finger domain

Authors

Alam, S.L., Higa, M.M., Ullman, K.S., Sundquist, W.I.

Assembly

2nd RAN binding zinc finger from hNUP153

Entity

1. hNUP153 (polymer, Thiol state: all other bound), 32 monomers, 3488.060 Da Detail

GHMVIGTWDC DTCLVQNKPE AIKCVACETP KP

2. ZN (non-polymer), 65.409 Da

Total weight

3553.469 Da

Max. entity weight

3488.06 Da

Entity Connection

na 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS10:SG	2:ZN1:ZN
2	na	sing	1:CYS13:SG	2:ZN1:ZN
3	na	sing	1:CYS24:SG	2:ZN1:ZN
4	na	sing	1:CYS27:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.0 %, Completeness (bb): 96.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	98.0 % (348 of 355)	98.9 % (182 of 184)	97.8 % (136 of 139)	93.8 % (30 of 32)
Backbone	96.2 % (179 of 186)	96.8 % (61 of 63)	96.8 % (91 of 94)	93.1 % (27 of 29)
Sidechain	100.0 % (199 of 199)	100.0 % (121 of 121)	100.0 % (75 of 75)	100.0 % (3 of 3)
Aromatic	100.0 % (16 of 16)	100.0 % (8 of 8)	100.0 % (7 of 7)	100.0 % (1 of 1)
Methyl	100.0 % (34 of 34)	100.0 % (17 of 17)	100.0 % (17 of 17)

1. hNUP153

GHMVIGTWDC DTCLVQNKPE AIKCVACETP KP

Show sample condition

Sample

Pressure 1.0 (±0.1) atm, Temperature 293 (±1) K, Details 15N labeled sample

#	Name	Isotope labeling	Type	Concentration
1	hNUP153	[U-95% 15N]	protein	1.5 (±0.1) mM
2	tris buffer		buffer	20 (±0.01) mM
3	NaCl		salt	50 (±0.01) mM
4	BME		reducing agent	5 (±0.01) mM
5	ZnCl2		salt	10 (±0.01) uM

Protein Blocks Logo

Calculated from 20 models in PDB: 2GQE, Strand ID: A Detail

Release date

2007-04-18

Citation

Molecular characterization of the Ran-binding zinc finger domain of Nup153
Higa, M.M., Alam, S.L., Sundquist, W.I., Ullman, K.S.
J. Biol. Chem. (2007), 282, 17090-17100, PubMed 17426026 , DOI 10.1074/jbc.M702715200 , Abstract

Related entities 1. hNUP153, : 1 : 3 : 153 entities Detail

Interaction partners 1. hNUP153, : 39 interactors Detail

More details for 39 interactors identified by 24 citations

Experiments performed 17 experiments Detail

1 1H15N_HSQC

Instrument

Model Varian INOVA (600 MHz)

Sample #1

pH 7.2 (±0.1), Details 15N labeled sample

#	Name	Isotope labeling	Type	Concentration
1	hNUP153	[U-95% 15N]	protein	1.5 (±0.1) mM
2	tris buffer		buffer	20 (±0.01) mM
3	NaCl		salt	50 (±0.01) mM
4	BME		reducing agent	5 (±0.01) mM
5	ZnCl2		salt	10 (±0.01) uM

Sample #2

pH 7.2 (±0.1), Details 13C 15N labled sample

#	Name	Isotope labeling	Type	Concentration
6	hNUP153	[U-95% 13C; U-95% 15N]	protein	1.0 (±0.1) mM
7	tris buffer		buffer	20 (±0.01) mM
8	NaCl		salt	50 (±0.01) mM
9	BME		reducing agent	5 (±0.01) mM
10	ZnCl2		salt	10 (±0.01) uM

2 1H13C HSQC

Instrument

Model Varian INOVA (600 MHz)

Sample #1

pH 7.2 (±0.1), Details 15N labeled sample

#	Name	Isotope labeling	Type	Concentration
1	hNUP153	[U-95% 15N]	protein	1.5 (±0.1) mM
2	tris buffer		buffer	20 (±0.01) mM
3	NaCl		salt	50 (±0.01) mM
4	BME		reducing agent	5 (±0.01) mM
5	ZnCl2		salt	10 (±0.01) uM

Sample #2

pH 7.2 (±0.1), Details 13C 15N labled sample

#	Name	Isotope labeling	Type	Concentration
6	hNUP153	[U-95% 13C; U-95% 15N]	protein	1.0 (±0.1) mM
7	tris buffer		buffer	20 (±0.01) mM
8	NaCl		salt	50 (±0.01) mM
9	BME		reducing agent	5 (±0.01) mM
10	ZnCl2		salt	10 (±0.01) uM

3 HNCA

Instrument

Model Varian INOVA (600 MHz)

Sample #1

pH 7.2 (±0.1), Details 15N labeled sample

#	Name	Isotope labeling	Type	Concentration
1	hNUP153	[U-95% 15N]	protein	1.5 (±0.1) mM
2	tris buffer		buffer	20 (±0.01) mM
3	NaCl		salt	50 (±0.01) mM
4	BME		reducing agent	5 (±0.01) mM
5	ZnCl2		salt	10 (±0.01) uM

Sample #2

pH 7.2 (±0.1), Details 13C 15N labled sample

#	Name	Isotope labeling	Type	Concentration
6	hNUP153	[U-95% 13C; U-95% 15N]	protein	1.0 (±0.1) mM
7	tris buffer		buffer	20 (±0.01) mM
8	NaCl		salt	50 (±0.01) mM
9	BME		reducing agent	5 (±0.01) mM
10	ZnCl2		salt	10 (±0.01) uM

4 HNCO

Instrument

Model Varian INOVA (600 MHz)

Sample #1

pH 7.2 (±0.1), Details 15N labeled sample

#	Name	Isotope labeling	Type	Concentration
1	hNUP153	[U-95% 15N]	protein	1.5 (±0.1) mM
2	tris buffer		buffer	20 (±0.01) mM
3	NaCl		salt	50 (±0.01) mM
4	BME		reducing agent	5 (±0.01) mM
5	ZnCl2		salt	10 (±0.01) uM

Sample #2

pH 7.2 (±0.1), Details 13C 15N labled sample

#	Name	Isotope labeling	Type	Concentration
6	hNUP153	[U-95% 13C; U-95% 15N]	protein	1.0 (±0.1) mM
7	tris buffer		buffer	20 (±0.01) mM
8	NaCl		salt	50 (±0.01) mM
9	BME		reducing agent	5 (±0.01) mM
10	ZnCl2		salt	10 (±0.01) uM

5 HC(CO)CA

Instrument

Model Varian INOVA (600 MHz)

Sample #1

pH 7.2 (±0.1), Details 15N labeled sample

#	Name	Isotope labeling	Type	Concentration
1	hNUP153	[U-95% 15N]	protein	1.5 (±0.1) mM
2	tris buffer		buffer	20 (±0.01) mM
3	NaCl		salt	50 (±0.01) mM
4	BME		reducing agent	5 (±0.01) mM
5	ZnCl2		salt	10 (±0.01) uM

Sample #2

pH 7.2 (±0.1), Details 13C 15N labled sample

#	Name	Isotope labeling	Type	Concentration
6	hNUP153	[U-95% 13C; U-95% 15N]	protein	1.0 (±0.1) mM
7	tris buffer		buffer	20 (±0.01) mM
8	NaCl		salt	50 (±0.01) mM
9	BME		reducing agent	5 (±0.01) mM
10	ZnCl2		salt	10 (±0.01) uM

6 HNCACB

Instrument

Model Varian INOVA (600 MHz)

Sample #1

pH 7.2 (±0.1), Details 15N labeled sample

#	Name	Isotope labeling	Type	Concentration
1	hNUP153	[U-95% 15N]	protein	1.5 (±0.1) mM
2	tris buffer		buffer	20 (±0.01) mM
3	NaCl		salt	50 (±0.01) mM
4	BME		reducing agent	5 (±0.01) mM
5	ZnCl2		salt	10 (±0.01) uM

Sample #2

pH 7.2 (±0.1), Details 13C 15N labled sample

#	Name	Isotope labeling	Type	Concentration
6	hNUP153	[U-95% 13C; U-95% 15N]	protein	1.0 (±0.1) mM
7	tris buffer		buffer	20 (±0.01) mM
8	NaCl		salt	50 (±0.01) mM
9	BME		reducing agent	5 (±0.01) mM
10	ZnCl2		salt	10 (±0.01) uM

7 CBCAC(CO)NH

Instrument

Model Varian INOVA (600 MHz)

Sample #1

pH 7.2 (±0.1), Details 15N labeled sample

#	Name	Isotope labeling	Type	Concentration
1	hNUP153	[U-95% 15N]	protein	1.5 (±0.1) mM
2	tris buffer		buffer	20 (±0.01) mM
3	NaCl		salt	50 (±0.01) mM
4	BME		reducing agent	5 (±0.01) mM
5	ZnCl2		salt	10 (±0.01) uM

Sample #2

pH 7.2 (±0.1), Details 13C 15N labled sample

#	Name	Isotope labeling	Type	Concentration
6	hNUP153	[U-95% 13C; U-95% 15N]	protein	1.0 (±0.1) mM
7	tris buffer		buffer	20 (±0.01) mM
8	NaCl		salt	50 (±0.01) mM
9	BME		reducing agent	5 (±0.01) mM
10	ZnCl2		salt	10 (±0.01) uM

8 H(CCO)NH-TOCSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

pH 7.2 (±0.1), Details 15N labeled sample

#	Name	Isotope labeling	Type	Concentration
1	hNUP153	[U-95% 15N]	protein	1.5 (±0.1) mM
2	tris buffer		buffer	20 (±0.01) mM
3	NaCl		salt	50 (±0.01) mM
4	BME		reducing agent	5 (±0.01) mM
5	ZnCl2		salt	10 (±0.01) uM

Sample #2

pH 7.2 (±0.1), Details 13C 15N labled sample

#	Name	Isotope labeling	Type	Concentration
6	hNUP153	[U-95% 13C; U-95% 15N]	protein	1.0 (±0.1) mM
7	tris buffer		buffer	20 (±0.01) mM
8	NaCl		salt	50 (±0.01) mM
9	BME		reducing agent	5 (±0.01) mM
10	ZnCl2		salt	10 (±0.01) uM

9 HCCH-TOCSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

pH 7.2 (±0.1), Details 15N labeled sample

#	Name	Isotope labeling	Type	Concentration
1	hNUP153	[U-95% 15N]	protein	1.5 (±0.1) mM
2	tris buffer		buffer	20 (±0.01) mM
3	NaCl		salt	50 (±0.01) mM
4	BME		reducing agent	5 (±0.01) mM
5	ZnCl2		salt	10 (±0.01) uM

Sample #2

pH 7.2 (±0.1), Details 13C 15N labled sample

#	Name	Isotope labeling	Type	Concentration
6	hNUP153	[U-95% 13C; U-95% 15N]	protein	1.0 (±0.1) mM
7	tris buffer		buffer	20 (±0.01) mM
8	NaCl		salt	50 (±0.01) mM
9	BME		reducing agent	5 (±0.01) mM
10	ZnCl2		salt	10 (±0.01) uM

10 (H)C(CO)NH-TOCSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

pH 7.2 (±0.1), Details 15N labeled sample

#	Name	Isotope labeling	Type	Concentration
1	hNUP153	[U-95% 15N]	protein	1.5 (±0.1) mM
2	tris buffer		buffer	20 (±0.01) mM
3	NaCl		salt	50 (±0.01) mM
4	BME		reducing agent	5 (±0.01) mM
5	ZnCl2		salt	10 (±0.01) uM

Sample #2

pH 7.2 (±0.1), Details 13C 15N labled sample

#	Name	Isotope labeling	Type	Concentration
6	hNUP153	[U-95% 13C; U-95% 15N]	protein	1.0 (±0.1) mM
7	tris buffer		buffer	20 (±0.01) mM
8	NaCl		salt	50 (±0.01) mM
9	BME		reducing agent	5 (±0.01) mM
10	ZnCl2		salt	10 (±0.01) uM

11 HNHA

Instrument

Model Varian INOVA (600 MHz)

Sample #1

pH 7.2 (±0.1), Details 15N labeled sample

#	Name	Isotope labeling	Type	Concentration
1	hNUP153	[U-95% 15N]	protein	1.5 (±0.1) mM
2	tris buffer		buffer	20 (±0.01) mM
3	NaCl		salt	50 (±0.01) mM
4	BME		reducing agent	5 (±0.01) mM
5	ZnCl2		salt	10 (±0.01) uM

Sample #2

pH 7.2 (±0.1), Details 13C 15N labled sample

#	Name	Isotope labeling	Type	Concentration
6	hNUP153	[U-95% 13C; U-95% 15N]	protein	1.0 (±0.1) mM
7	tris buffer		buffer	20 (±0.01) mM
8	NaCl		salt	50 (±0.01) mM
9	BME		reducing agent	5 (±0.01) mM
10	ZnCl2		salt	10 (±0.01) uM

12 HNHB

Instrument

Model Varian INOVA (600 MHz)

Sample #1

pH 7.2 (±0.1), Details 15N labeled sample

#	Name	Isotope labeling	Type	Concentration
1	hNUP153	[U-95% 15N]	protein	1.5 (±0.1) mM
2	tris buffer		buffer	20 (±0.01) mM
3	NaCl		salt	50 (±0.01) mM
4	BME		reducing agent	5 (±0.01) mM
5	ZnCl2		salt	10 (±0.01) uM

Sample #2

pH 7.2 (±0.1), Details 13C 15N labled sample

#	Name	Isotope labeling	Type	Concentration
6	hNUP153	[U-95% 13C; U-95% 15N]	protein	1.0 (±0.1) mM
7	tris buffer		buffer	20 (±0.01) mM
8	NaCl		salt	50 (±0.01) mM
9	BME		reducing agent	5 (±0.01) mM
10	ZnCl2		salt	10 (±0.01) uM

13 HN(CO)HB

Instrument

Model Varian INOVA (600 MHz)

Sample #1

pH 7.2 (±0.1), Details 15N labeled sample

#	Name	Isotope labeling	Type	Concentration
1	hNUP153	[U-95% 15N]	protein	1.5 (±0.1) mM
2	tris buffer		buffer	20 (±0.01) mM
3	NaCl		salt	50 (±0.01) mM
4	BME		reducing agent	5 (±0.01) mM
5	ZnCl2		salt	10 (±0.01) uM

Sample #2

pH 7.2 (±0.1), Details 13C 15N labled sample

#	Name	Isotope labeling	Type	Concentration
6	hNUP153	[U-95% 13C; U-95% 15N]	protein	1.0 (±0.1) mM
7	tris buffer		buffer	20 (±0.01) mM
8	NaCl		salt	50 (±0.01) mM
9	BME		reducing agent	5 (±0.01) mM
10	ZnCl2		salt	10 (±0.01) uM

14 LRCC

Instrument

Model Varian INOVA (600 MHz)

Sample #1

pH 7.2 (±0.1), Details 15N labeled sample

#	Name	Isotope labeling	Type	Concentration
1	hNUP153	[U-95% 15N]	protein	1.5 (±0.1) mM
2	tris buffer		buffer	20 (±0.01) mM
3	NaCl		salt	50 (±0.01) mM
4	BME		reducing agent	5 (±0.01) mM
5	ZnCl2		salt	10 (±0.01) uM

Sample #2

pH 7.2 (±0.1), Details 13C 15N labled sample

#	Name	Isotope labeling	Type	Concentration
6	hNUP153	[U-95% 13C; U-95% 15N]	protein	1.0 (±0.1) mM
7	tris buffer		buffer	20 (±0.01) mM
8	NaCl		salt	50 (±0.01) mM
9	BME		reducing agent	5 (±0.01) mM
10	ZnCl2		salt	10 (±0.01) uM

15 1H13C 3D-NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

pH 7.2 (±0.1), Details 15N labeled sample

#	Name	Isotope labeling	Type	Concentration
1	hNUP153	[U-95% 15N]	protein	1.5 (±0.1) mM
2	tris buffer		buffer	20 (±0.01) mM
3	NaCl		salt	50 (±0.01) mM
4	BME		reducing agent	5 (±0.01) mM
5	ZnCl2		salt	10 (±0.01) uM

Sample #2

pH 7.2 (±0.1), Details 13C 15N labled sample

#	Name	Isotope labeling	Type	Concentration
6	hNUP153	[U-95% 13C; U-95% 15N]	protein	1.0 (±0.1) mM
7	tris buffer		buffer	20 (±0.01) mM
8	NaCl		salt	50 (±0.01) mM
9	BME		reducing agent	5 (±0.01) mM
10	ZnCl2		salt	10 (±0.01) uM

16 1H15N 3D NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

pH 7.2 (±0.1), Details 15N labeled sample

#	Name	Isotope labeling	Type	Concentration
1	hNUP153	[U-95% 15N]	protein	1.5 (±0.1) mM
2	tris buffer		buffer	20 (±0.01) mM
3	NaCl		salt	50 (±0.01) mM
4	BME		reducing agent	5 (±0.01) mM
5	ZnCl2		salt	10 (±0.01) uM

Sample #2

pH 7.2 (±0.1), Details 13C 15N labled sample

#	Name	Isotope labeling	Type	Concentration
6	hNUP153	[U-95% 13C; U-95% 15N]	protein	1.0 (±0.1) mM
7	tris buffer		buffer	20 (±0.01) mM
8	NaCl		salt	50 (±0.01) mM
9	BME		reducing agent	5 (±0.01) mM
10	ZnCl2		salt	10 (±0.01) uM

17 HCCH-COSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

pH 7.2 (±0.1), Details 15N labeled sample

#	Name	Isotope labeling	Type	Concentration
1	hNUP153	[U-95% 15N]	protein	1.5 (±0.1) mM
2	tris buffer		buffer	20 (±0.01) mM
3	NaCl		salt	50 (±0.01) mM
4	BME		reducing agent	5 (±0.01) mM
5	ZnCl2		salt	10 (±0.01) uM

Sample #2

pH 7.2 (±0.1), Details 13C 15N labled sample

#	Name	Isotope labeling	Type	Concentration
6	hNUP153	[U-95% 13C; U-95% 15N]	protein	1.0 (±0.1) mM
7	tris buffer		buffer	20 (±0.01) mM
8	NaCl		salt	50 (±0.01) mM
9	BME		reducing agent	5 (±0.01) mM
10	ZnCl2		salt	10 (±0.01) uM

nullKeywords RAN, zinc finger, NMR, nucleoporin, nucleus, NUP153, NUP358, Ran, RanBP2, structure, transport, nuclear pore, pore

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Found 1 Document (2.77 seconds)

BMRB: 7158

Sample

Related entities 1. hNUP153, : 1 : 3 : 153 entities Detail

Interaction partners 1. hNUP153, : 39 interactors Detail

Experiments performed 17 experiments Detail

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