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BMRB: 7170

2HC5

1H, 13C, and 15N Chemical Shift Assignments for Bacillus subtilis Hypothetical Protein yvyC: Northeast Structural Genomics Consortium target SR482

Authors

Eletsky, A., Liu, G., Atreya, H.S., Sukumaran, D., Wang, D., Cunningham, K., Janjua, H., Ma, L., Xiao, R., Liu, J., Baran, M., Acton, T.B., Rost, B., Montelione, G.T., Szyperski, T.

Assembly

sr482 monomer

Entity

1. sr482 monomer (polymer, Thiol state: not present), 117 monomers, 14071.65 Da Detail

LNIERLTTLQ PVWDRYDTQI HNQKDNDNEV PVHQVSYTNL AEMVGEMNKL LEPSQVHLKF ELHDKLNEYY VKVIEDSTNE VIREIPPKRW LDFYAAMTEF LGLFVDEKKL EHHHHHH

Formula weight

14071.65 Da

Source organism

Bacillus subtilis

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 91.5 %, Completeness (bb): 93.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.5 % (1322 of 1445)	92.0 % (693 of 753)	89.9 % (508 of 565)	95.3 % (121 of 127)
Backbone	93.1 % (644 of 692)	91.8 % (212 of 231)	93.4 % (326 of 349)	94.6 % (106 of 112)
Sidechain	90.7 % (787 of 868)	92.1 % (481 of 522)	87.9 % (291 of 331)	100.0 % (15 of 15)
Aromatic	66.7 % (96 of 144)	79.2 % (57 of 72)	52.9 % (37 of 70)	100.0 % (2 of 2)
Methyl	97.7 % (127 of 130)	96.9 % (63 of 65)	98.5 % (64 of 65)

1. sr482

LNIERLTTLQ PVWDRYDTQI HNQKDNDNEV PVHQVSYTNL AEMVGEMNKL LEPSQVHLKF ELHDKLNEYY VKVIEDSTNE VIREIPPKRW LDFYAAMTEF LGLFVDEKKL EHHHHHH

Show sample condition

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	sr482	[U-100% 13C; U-100% 15N]	protein	1.1 mM
2	Tris		buffer	10 mM
3	NaCl		salt	100 mM
4	NaN3			0.02 % w/w

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	sr482	[U-5% 13C; U-100% 15N]	protein	1.1 mM
6	Tris		buffer	10 mM
7	NaCl		salt	100 mM
8	NaN3			0.02 % w/w

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 5 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 5 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2HC5, Strand ID: A Detail

Release date

2015-04-22

Citation

Solution NMR Structure of Bacillus subtilis Protein yvyC
Eletsky, A., Liu, G., Atreya, H.S., Sukumaran, D., Wang, D., Cunningham, K., Janjua, H., Ma, L., Xiao, R., Liu, J., Baran, M., Acton, T.B., Rost, B., Montelione, G.T., Szyperski, T.
Proteins: Struct. Funct. Genet.

Related entities 1. sr482 monomer, : 1 : 1 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H15N_HSQC

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	sr482	[U-100% 13C; U-100% 15N]	protein	1.1 mM
2	Tris		buffer	10 mM
3	NaCl		salt	100 mM
4	NaN3			0.02 % w/w

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	sr482	[U-5% 13C; U-100% 15N]	protein	1.1 mM
6	Tris		buffer	10 mM
7	NaCl		salt	100 mM
8	NaN3			0.02 % w/w

2 2D 1H13C_HSQC (ali)

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	sr482	[U-100% 13C; U-100% 15N]	protein	1.1 mM
2	Tris		buffer	10 mM
3	NaCl		salt	100 mM
4	NaN3			0.02 % w/w

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	sr482	[U-5% 13C; U-100% 15N]	protein	1.1 mM
6	Tris		buffer	10 mM
7	NaCl		salt	100 mM
8	NaN3			0.02 % w/w

3 2D 1H13C_HSQC (aro)

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	sr482	[U-100% 13C; U-100% 15N]	protein	1.1 mM
2	Tris		buffer	10 mM
3	NaCl		salt	100 mM
4	NaN3			0.02 % w/w

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	sr482	[U-5% 13C; U-100% 15N]	protein	1.1 mM
6	Tris		buffer	10 mM
7	NaCl		salt	100 mM
8	NaN3			0.02 % w/w

4 2D CT-1H13C_HSQC (ali)

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	sr482	[U-100% 13C; U-100% 15N]	protein	1.1 mM
2	Tris		buffer	10 mM
3	NaCl		salt	100 mM
4	NaN3			0.02 % w/w

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	sr482	[U-5% 13C; U-100% 15N]	protein	1.1 mM
6	Tris		buffer	10 mM
7	NaCl		salt	100 mM
8	NaN3			0.02 % w/w

5 2D CT-1H13C_HSQC (aro)

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	sr482	[U-100% 13C; U-100% 15N]	protein	1.1 mM
2	Tris		buffer	10 mM
3	NaCl		salt	100 mM
4	NaN3			0.02 % w/w

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	sr482	[U-5% 13C; U-100% 15N]	protein	1.1 mM
6	Tris		buffer	10 mM
7	NaCl		salt	100 mM
8	NaN3			0.02 % w/w

6 3D HNCO

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	sr482	[U-100% 13C; U-100% 15N]	protein	1.1 mM
2	Tris		buffer	10 mM
3	NaCl		salt	100 mM
4	NaN3			0.02 % w/w

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	sr482	[U-5% 13C; U-100% 15N]	protein	1.1 mM
6	Tris		buffer	10 mM
7	NaCl		salt	100 mM
8	NaN3			0.02 % w/w

7 (4,3)D GFT CABCA(CO)NHN

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	sr482	[U-100% 13C; U-100% 15N]	protein	1.1 mM
2	Tris		buffer	10 mM
3	NaCl		salt	100 mM
4	NaN3			0.02 % w/w

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	sr482	[U-5% 13C; U-100% 15N]	protein	1.1 mM
6	Tris		buffer	10 mM
7	NaCl		salt	100 mM
8	NaN3			0.02 % w/w

8 (4,3)D GFT HNNCABCA

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	sr482	[U-100% 13C; U-100% 15N]	protein	1.1 mM
2	Tris		buffer	10 mM
3	NaCl		salt	100 mM
4	NaN3			0.02 % w/w

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	sr482	[U-5% 13C; U-100% 15N]	protein	1.1 mM
6	Tris		buffer	10 mM
7	NaCl		salt	100 mM
8	NaN3			0.02 % w/w

9 (4,3)D GFT HABCAB(CO)NHN

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	sr482	[U-100% 13C; U-100% 15N]	protein	1.1 mM
2	Tris		buffer	10 mM
3	NaCl		salt	100 mM
4	NaN3			0.02 % w/w

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	sr482	[U-5% 13C; U-100% 15N]	protein	1.1 mM
6	Tris		buffer	10 mM
7	NaCl		salt	100 mM
8	NaN3			0.02 % w/w

10 (4,3)D GFT HCCH-COSY (ali)

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	sr482	[U-100% 13C; U-100% 15N]	protein	1.1 mM
2	Tris		buffer	10 mM
3	NaCl		salt	100 mM
4	NaN3			0.02 % w/w

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	sr482	[U-5% 13C; U-100% 15N]	protein	1.1 mM
6	Tris		buffer	10 mM
7	NaCl		salt	100 mM
8	NaN3			0.02 % w/w

11 (4,3)D GFT HCCH-COSY (aro)

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	sr482	[U-100% 13C; U-100% 15N]	protein	1.1 mM
2	Tris		buffer	10 mM
3	NaCl		salt	100 mM
4	NaN3			0.02 % w/w

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	sr482	[U-5% 13C; U-100% 15N]	protein	1.1 mM
6	Tris		buffer	10 mM
7	NaCl		salt	100 mM
8	NaN3			0.02 % w/w

12 3D 15N-, 13Cali-, 13Caro-resolved NOESY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	sr482	[U-100% 13C; U-100% 15N]	protein	1.1 mM
2	Tris		buffer	10 mM
3	NaCl		salt	100 mM
4	NaN3			0.02 % w/w

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	sr482	[U-5% 13C; U-100% 15N]	protein	1.1 mM
6	Tris		buffer	10 mM
7	NaCl		salt	100 mM
8	NaN3			0.02 % w/w

13 2D CT-1H13C_HSQC (methyl)

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (750 MHz)

Sample #1

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	sr482	[U-100% 13C; U-100% 15N]	protein	1.1 mM
2	Tris		buffer	10 mM
3	NaCl		salt	100 mM
4	NaN3			0.02 % w/w

Sample #2

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	sr482	[U-5% 13C; U-100% 15N]	protein	1.1 mM
6	Tris		buffer	10 mM
7	NaCl		salt	100 mM
8	NaN3			0.02 % w/w

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Found 1 Document (4.848 seconds)

BMRB: 7170

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. sr482 monomer, : 1 : 1 entities Detail

Experiments performed 13 experiments Detail

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