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Found 1 Document (5.811 seconds)

BMRB: 7178

2GZP

Solution NMR structure of Q8ZP25 from Salmonella typhimurium LT2.

Authors

Parish, D., Liu, G., Shen, Y., Ho, C., Cunningham, K., Xiao, R., Swapna, G.V.T., Acton, T.B., Montelione, G.T., Szyperski, T.

Assembly

putative chaperone

Entity

1. putative chaperone (polymer, Thiol state: not present), 142 monomers, 16126.01 Da Detail

MANDTPFSAL WQRLLTRGWQ PVEASTVDDW IKRVGDGVIL LSSDPRRTPE VSDNPVMIAE LLREFPQFDW QVAVADLEQS EAIGDRFNVR RFPATLVFTD GKLRGALSGI HPWAELLTLM RSIVDTPAAQ ETVQLEHHHH HH

Formula weight

16126.01 Da

Source organism

Salmonella enterica subsp. enterica serovar Typhimurium

Exptl. method

NMR

Refine. method

constrained simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 73.9 %, Completeness: 64.4 %, Completeness (bb): 59.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	64.4 % (1065 of 1653)	69.4 % (592 of 853)	57.1 % (372 of 652)	68.2 % (101 of 148)
Backbone	59.7 % (498 of 834)	70.9 % (200 of 282)	49.2 % (206 of 419)	69.2 % (92 of 133)
Sidechain	69.3 % (661 of 954)	68.7 % (392 of 571)	70.7 % (260 of 368)	60.0 % (9 of 15)
Aromatic	64.9 % (96 of 148)	67.6 % (50 of 74)	59.4 % (41 of 69)	100.0 % (5 of 5)
Methyl	84.5 % (147 of 174)	85.1 % (74 of 87)	83.9 % (73 of 87)

1. putative chaperone

MANDTPFSAL WQRLLTRGWQ PVEASTVDDW IKRVGDGVIL LSSDPRRTPE VSDNPVMIAE LLREFPQFDW QVAVADLEQS EAIGDRFNVR RFPATLVFTD GKLRGALSGI HPWAELLTLM RSIVDTPAAQ ETVQLEHHHH HH

Show sample condition

Sample

Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	putative chaperone	[U-13C; U-15N]	1 mM
2	H20		95 %
3	D20		5 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2GZP, Strand ID: A Detail

Release date

2007-09-11

Citation

Solution NMR structure of Q8ZP25 from Salmonella typhimurium LT2
Parish, D., Liu, G., Shen, Y., Ho, C.K., Cunningham, K., Xiao, R., Swapna, G.V.T., Acton, T.B., Montelione, G.T., Szyperski, T.

Related entities 1. putative chaperone, : 1 : 1 : 1 : 8 entities Detail

Experiments performed 7 experiments Detail

1 Brunger, Adams, Clore, DeLano, Gros, Grosse-Kunstleve, Jiang, Kuszewski, Nilges, Pannu, Read, Rice, Simonson, Warren.

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	putative chaperone	[U-13C; U-15N]	1 mM
2	H20		95 %
3	D20		5 %

Sample #2

Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
4	putative chaperone	[U-5% 13C; U-15N]	1 mM
5	H20		95 %
6	D20		5 %

2 Guntert

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	putative chaperone	[U-13C; U-15N]	1 mM
2	H20		95 %
3	D20		5 %

Sample #2

Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
4	putative chaperone	[U-5% 13C; U-15N]	1 mM
5	H20		95 %
6	D20		5 %

3 Varian

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	putative chaperone	[U-13C; U-15N]	1 mM
2	H20		95 %
3	D20		5 %

Sample #2

Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
4	putative chaperone	[U-5% 13C; U-15N]	1 mM
5	H20		95 %
6	D20		5 %

4 Delaglio, Bax

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	putative chaperone	[U-13C; U-15N]	1 mM
2	H20		95 %
3	D20		5 %

Sample #2

Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
4	putative chaperone	[U-5% 13C; U-15N]	1 mM
5	H20		95 %
6	D20		5 %

5 Glaser

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	putative chaperone	[U-13C; U-15N]	1 mM
2	H20		95 %
3	D20		5 %

Sample #2

Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
4	putative chaperone	[U-5% 13C; U-15N]	1 mM
5	H20		95 %
6	D20		5 %

6 Huang, Tejero, Power, Montelione

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	putative chaperone	[U-13C; U-15N]	1 mM
2	H20		95 %
3	D20		5 %

Sample #2

Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
4	putative chaperone	[U-5% 13C; U-15N]	1 mM
5	H20		95 %
6	D20		5 %

7 Zimmerman, Moseley, Kiriyeva, Kulikowski, Montelione

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	putative chaperone	[U-13C; U-15N]	1 mM
2	H20		95 %
3	D20		5 %

Sample #2

Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
4	putative chaperone	[U-5% 13C; U-15N]	1 mM
5	H20		95 %
6	D20		5 %

nullKeywords GFT-NMR, NESG, Northeast Structural Genomics Consortium, Protein Structure Initiative, PSI, Putative thiol-disulfide isomerase, Structural Genomics

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Found 1 Document (5.811 seconds)

BMRB: 7178

Sample

Related entities 1. putative chaperone, : 1 : 1 : 1 : 8 entities Detail

Experiments performed 7 experiments Detail

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