BMRB: 7181

Solution Structure of Hypothetical protein PA4359: Northeast Structural Genomics Target PaT89

Authors

Zhang, Q., Liu, G., Yee, A., Arrowsmith, C., Szyperski, T.

Assembly

Hypothetical protein PA4359

Entity

1. Hypothetical protein PA4359 (polymer, Thiol state: not present), 75 monomers, 8251.594 Da Detail

MSALQPSRSY RITGYSPAIS NGYRQRLFSM GLLPGAALRV VRIAPLGDPI QVETRQTSLA LRRKDLALLT LVPLD

Formula weight

8251.594 Da

Source organism

Pseudomonas aeruginosa

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing, molecular dynamics, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.0 %, Completeness: 86.3 %, Completeness (bb): 81.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	86.3 % (752 of 871)	93.2 % (424 of 455)	76.3 % (261 of 342)	90.5 % (67 of 74)
Backbone	81.1 % (355 of 438)	94.6 % (141 of 149)	68.2 % (150 of 220)	92.8 % (64 of 69)
Sidechain	91.8 % (462 of 503)	92.5 % (283 of 306)	91.7 % (176 of 192)	60.0 % (3 of 5)
Aromatic	76.5 % (26 of 34)	100.0 % (17 of 17)	52.9 % (9 of 17)
Methyl	100.0 % (106 of 106)	100.0 % (53 of 53)	100.0 % (53 of 53)

1. Hypothetical protein PA4359

MSALQPSRSY RITGYSPAIS NGYRQRLFSM GLLPGAALRV VRIAPLGDPI QVETRQTSLA LRRKDLALLT LVPLD

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	Hypothetical protein PA4359	[U-13C; U-15N]		0.7 mM
2	tris			10 mM
3	NaCl			300 mM
4	glycerol			5 %
5	NaN3			0.01 %
6	benzamidiine			1 mM
7	H2O			90 %
8	D2O			10 %

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LACS Plot; CA

Referencing offset: 0.23 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: 0.23 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 2H3J, Strand ID: A Detail

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Release date

2006-11-07

Citation

Solution Structure of Hypothetical protein PA4359: Northest Structural Genomics Target PaT89
Zhang, Q., Liu, G., Yee, A., Arrowsmith, C., Szyperski, T.

Related entities 1. Hypothetical protein PA4359, : 1 : 7 entities Detail

Experiments performed 5 experiments Detail