BMRB: 7182

NMR Structure of YcgL, a Conserved Protein from Escherichia coli Representing the DUF709 Family with a Novel a/b/a Sandwich Fold

Authors

Minailiuc, O.M., Vavelyuk, O., Ekiel, I., Hung, M., Cygler, M., Gandhi, S.

Assembly

Hypothetical protein ycgL

Entity

1. Hypothetical protein ycgL (polymer, Thiol state: all free), 110 monomers, 12558.48 Da Detail

GSMPKPGILK SKSMFCVIYR SSKRDQTYLY VEKKDDFSRV PEELMKGFGQ PQLAMILPLD GRKKLVNADI EKVKQALTEQ GYYLQLPPPP EDLLKQHLSV MGQKTDDTNK

Formula weight

12558.48 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.5 %, Completeness: 79.3 %, Completeness (bb): 89.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	79.3 % (1069 of 1348)	73.7 % (529 of 718)	84.6 % (439 of 519)	91.0 % (101 of 111)
Backbone	89.7 % (576 of 642)	90.8 % (198 of 218)	87.6 % (283 of 323)	94.1 % (95 of 101)
Sidechain	71.8 % (581 of 809)	65.8 % (329 of 500)	82.3 % (246 of 299)	60.0 % (6 of 10)
Aromatic	74.3 % (55 of 74)	62.2 % (23 of 37)	86.5 % (32 of 37)
Methyl	99.1 % (105 of 106)	98.1 % (52 of 53)	100.0 % (53 of 53)

1. Hypothetical protein ycgL

GSMPKPGILK SKSMFCVIYR SSKRDQTYLY VEKKDDFSRV PEELMKGFGQ PQLAMILPLD GRKKLVNADI EKVKQALTEQ GYYLQLPPPP EDLLKQHLSV MGQKTDDTNK

Show sample condition

Sample

Pressure 1 atm, Temperature 308 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	Hypothetical protein ycgL
2	phosphate buffer			50 mM
3	DTT			15 mM
4	sodium azide			0.02 % w/v
5	D2O			10 %
6	H2O			90 %

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LACS Plot; CA

Referencing offset: 0.08 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: 0.08 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: -0.09 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CO

Referencing offset: 2.93 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 2H7A, Strand ID: A Detail

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Release date

2007-03-04

Citation

NMR structure of YcgL, a conserved protein from Escherichia coli representing the DUF709 family, with a novel alpha/beta/alpha sandwich fold
Minailiuc, O.M., Vavelyuk, O., Gandhi, S., Hung, M., Cygler, M., Ekiel, I.
Proteins (2007), 66, 1004-1007, PubMed 17221885 , DOI 10.1002/prot.21198 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Hypothetical protein ycgL, : 1 : 8 : 38 entities Detail

Interaction partners 1. Hypothetical protein ycgL, : 16 interactors Detail

Experiments performed 11 experiments Detail