BMRB: 7184

NMR solution structure of CSPsg4

Authors

Tomaselli, S., Crescenzi, O., Sanfelice, D., Ab, E., Tancredi, T., Picone, D.

Assembly

chemosensory protein CSP-sg4

Entity

1. chemosensory protein CSP-sg4 (polymer, Thiol state: all disulfide bound), 109 monomers, 12675.03 Da Detail

EEKYTTKYDN VNLDEILAND RLLNKYVQCL LEDDESNCTA DGKELKSVIP DALSNECAKC NEKQKEGTKK VLKHLINHKP DVWAQLKAKY DPDGTYSKKY EDREKELHQ

Formula weight

12675.03 Da

Entity Connection

disulfide 2 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS29:SG	1:CYS38:SG
2	disulfide	sing	1:CYS57:SG	1:CYS60:SG

Source organism

Schistocerca gregaria

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.2 %, Completeness (bb): 96.9 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.2 % (1164 of 1305)	87.8 % (606 of 690)	88.5 % (439 of 496)	100.0 % (119 of 119)
Backbone	96.9 % (628 of 648)	98.2 % (214 of 218)	95.1 % (308 of 324)	100.0 % (106 of 106)
Sidechain	83.7 % (639 of 763)	83.1 % (392 of 472)	84.2 % (234 of 278)	100.0 % (13 of 13)
Aromatic	33.3 % (24 of 72)	63.9 % (23 of 36)	0.0 % (0 of 35)	100.0 % (1 of 1)
Methyl	95.1 % (97 of 102)	96.1 % (49 of 51)	94.1 % (48 of 51)

1. chemosensory protein CSP-sg4

EEKYTTKYDN VNLDEILAND RLLNKYVQCL LEDDESNCTA DGKELKSVIP DALSNECAKC NEKQKEGTKK VLKHLINHKP DVWAQLKAKY DPDGTYSKKY EDREKELHQ

Show sample condition

Sample

Pressure 1 atm, Temperature 300 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	chemosensory protein CSP-sg4	[U-13C; U-15N]		1.2 mM
2	phosphate buffer			50 mM
3	D2O			5 %
4	H2O			95 %

Hide sample codition

LACS Plot; CA

Referencing offset: 2.27 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
15C	H2O	protons	0.0 ppm	internal	indirect	0.1013291
1H	H2O	protons	4.81616 ppm	internal	direct	1.0
13N	H2O	protons	0.0 ppm	internal	indirect	0.2514495

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: 2.27 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
15C	H2O	protons	0.0 ppm	internal	indirect	0.1013291
1H	H2O	protons	4.81616 ppm	internal	direct	1.0
13N	H2O	protons	0.0 ppm	internal	indirect	0.2514495

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
15C	H2O	protons	0.0 ppm	internal	indirect	0.1013291
1H	H2O	protons	4.81616 ppm	internal	direct	1.0
13N	H2O	protons	0.0 ppm	internal	indirect	0.2514495

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 2.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
15C	H2O	protons	0.0 ppm	internal	indirect	0.1013291
1H	H2O	protons	4.81616 ppm	internal	direct	1.0
13N	H2O	protons	0.0 ppm	internal	indirect	0.2514495

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 2GVS, Strand ID: A Detail

---

Release date

2006-07-13

Citation

Solution structure of a chemosensory protein from the desert locust Schistocerca gregaria
Tomaselli, S., Crescenzi, O., Sanfelice, D., Ab, E., Wechselberger, R., Angeli, S., Scaloni, A., Boelens, R., Tancredi, T., Pelosi, P., Picone, D.
Biochemistry (2006), 45, 10606-10613, PubMed 16939212 , DOI 10.1021/bi060998w , Abstract

Related entities 1. chemosensory protein CSP-sg4, : 1 : 14 entities Detail

Experiments performed 2 experiments Detail