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BMRB: 7185

2GUT

An ARC/MEDIATOR subunit required for SREBP gene activation and regulation of cholesterol and fatty acid homeostasis

Authors

Wagner, G., Vought, B.W., Sun, Y.Z., Hyberts, S.G.

Assembly

Positive cofactor 2 glutamine/Q-rich-associated protein

Entity

1. Positive cofactor 2 glutamine/Q-rich-associated protein (polymer, Thiol state: not present), 77 monomers, 8772.923 Da Detail

GAMGQETDWR STAFRQKLVS QIEDAMRKAG VAHSKSSKDM ESHVFLKAKT RDEYLSLVAR LIIHFRDIHN KKSQASV

Formula weight

8772.923 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing using CYANA

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 74.0 %, Completeness (bb): 69.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	74.0 % (672 of 908)	70.1 % (333 of 475)	74.9 % (262 of 350)	92.8 % (77 of 83)
Backbone	69.9 % (323 of 462)	58.0 % (91 of 157)	68.4 % (156 of 228)	98.7 % (76 of 77)
Sidechain	81.2 % (422 of 520)	76.1 % (242 of 318)	91.3 % (179 of 196)	16.7 % (1 of 6)
Aromatic	57.6 % (38 of 66)	57.6 % (19 of 33)	59.4 % (19 of 32)	0.0 % (0 of 1)
Methyl	97.4 % (76 of 78)	97.4 % (38 of 39)	97.4 % (38 of 39)

1. Positive cofactor 2 glutamine/Q-rich-associated protein

GAMGQETDWR STAFRQKLVS QIEDAMRKAG VAHSKSSKDM ESHVFLKAKT RDEYLSLVAR LIIHFRDIHN KKSQASV

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Positive cofactor 2 glutamine/Q-rich-associated protein	[U-15N; U-13C]	1 mM
2	NaCl		30 mM
3	phosphate buffer		10 mM
4	H2O		90 %
5	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: -0.3 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: -0.3 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	null	null
¹H	na	na	null	null
¹⁵N	na	na	null	null

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2GUT, Strand ID: A Detail

Release date

2006-10-10

Citation

An ARC/Mediator subunit required for SREBP control of cholesterol and lipid homeostasis
Yang, F., Vought, B.W., Satterlee, J.S., Walker, A.K., Jim_Sun, Z.Y., Watts, J.L., DeBeaumont, R., Saito, R.M., Hyberts, S.G., Yang, S., Macol, C., Iyer, L., Tjian, R., van den Heuvel, S., Hart, A.C., Wagner, G., Naar, A.M.
Nature (2006), 442, 700-704, PubMed 16799563 , DOI 10.1038/nature04942 , Abstract

Entries sharing articles Swiss-Prot: 2 entries Detail

  Swiss-Prot: Q21955 released on 2002-06-06
    Title MED15_CAEEL Entity Mediator of RNA polymerase II transcription subunit 15
  Swiss-Prot: Q96RN5 released on 2002-04-16
    Title MED15_HUMAN Entity Mediator of RNA polymerase II transcription subunit 15

Related entities 1. Positive cofactor 2 glutamine/Q-rich-associated protein, : 1 : 5 : 9 entities Detail

Interaction partners 1. Positive cofactor 2 glutamine/Q-rich-associated protein, : 48 interactors Detail

More details for 48 interactors identified by 17 citations

Experiments performed 14 experiments Detail

1 HNCA