BMRB: 7186

Backbone 1H Chemical Shift Assignments for Magi 5

Authors

Sabo, J.K., Corzo, G., Bosmans, F., Billen, B., Villegas, E., Tygat, J., Norton, R.S.

Assembly

Spider toxin Magi 5

Entity

1. Spider toxin Magi 5 (polymer, Thiol state: all disulfide bound), 29 monomers, 3293.027 Da Detail

GCKLTFWKCK NKKECCGWNA CALGICMPR

Formula weight

3293.027 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS2:SG	1:CYS16:SG
2	disulfide	sing	1:CYS9:SG	1:CYS21:SG
3	disulfide	sing	1:CYS15:SG	1:CYS26:SG

Source organism

Macrothele gigas

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 86.4 %, Completeness (bb): 96.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	86.4 % (159 of 184)	86.4 % (159 of 184)
Backbone	96.7 % (58 of 60)	96.7 % (58 of 60)
Sidechain	81.5 % (101 of 124)	81.5 % (101 of 124)
Aromatic	88.2 % (15 of 17)	88.2 % (15 of 17)
Methyl	100.0 % (9 of 9)	100.0 % (9 of 9)

1. Magi 5

GCKLTFWKCK NKKECCGWNA CALGICMPR

Show sample condition

Sample

Pressure 1 atm, Temperature 278 (±0.1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Magi 5			2.2 mM
2	H2O			95 %
3	D2O			5 %

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Calculated from 20 models in PDB: 2GX1, Strand ID: A Detail

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Coupling constant

14 J values in 1 lists
Pressure 1 atm, Temperature 278 (±0.1) K, pH 4.5 (±0.2) Detail

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Pressure 1 atm, Temperature 278 (±0.1) K, pH 4.5 (±0.2)

Experiment name 2D 1H-1H DQF-COSY, 2D 1H-1H NOESY, 2D 1H-1H TOCSY

List #1 J_values_set_1

Release date

2006-06-21

Citation

Solution structure and alanine scan of a spider toxin that affects the activation of mammalian voltage-gated sodium channels
Corzo, G., Sabo, J.K., Bosmans, F., Billen, B., Villegas, E., Tygat, J., Norton, R.S.
J. Biol. Chem. (2007), 282, 4643-4652, PubMed 17148449 , DOI 10.1074/jbc.M605403200 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Spider toxin Magi 5, : 1 : 2 : 5 entities Detail

Experiments performed 3 experiments Detail