Search

Transcription Factor IIIA zinc fingers 4-6 bound to 5S rRNA 55mer
Authors
Lee, B.M.
Assembly
TFIIIA zf46 & 5S rRNA complex
Entity
1. 5S rRNA 55mer (polymer, Thiol state: not present), 55 monomers, 17688.40 Da Detail

GGGCCACACC UCUUGGGCCU GGUUAGUACC UGUUCGCUGG GAAUACCAGG UGCCC


2. TFIIIA zf46 (polymer, Thiol state: free and other bound), 87 monomers, 10276.65 Da Detail

MYVCHFENCG KAFKKHNQLK VHQFSHTQQL PYECPHEGCD KRFSLPSRLK RHEKVHAGYP CKKDDSCSFV GKTWTLYLKH VAECHQD


3. ZN (non-polymer), 65.409 × 3 Da
Total weight
28161.277 Da
Max. entity weight
17688.4 Da
Entity Connection
na 12 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1nasing2:CYS4:SG3:ZN1:ZN
2nasing2:CYS9:SG3:ZN1:ZN
3nasing2:HIS22:NE23:ZN1:ZN
4nasing2:HIS26:NE23:ZN1:ZN
5nasing2:CYS34:SG3:ZN1:ZN
6nasing2:CYS39:SG3:ZN1:ZN
7nasing2:HIS52:NE23:ZN1:ZN
8nasing2:HIS56:NE23:ZN1:ZN
9nasing2:CYS61:SG3:ZN1:ZN
10nasing2:CYS67:SG3:ZN1:ZN
11nasing2:HIS80:NE23:ZN1:ZN
12nasing2:HIS85:NE23:ZN1:ZN

Source organism
Xenopus laevis
Exptl. method
NMR
Refine. method
torsion angle dynamics, molecular dynamics, generalized Born solvation model
Data set
assigned_chemical_shifts, RDCs
Chem. Shift Complete
Sequence coverage: 61.3 %, Completeness: 46.9 %, Completeness (bb): 38.1 % Detail

Polymer type: polyribonucleotide polypeptide(L)

Total1H13C15N
All46.9 % (912 of 1946)49.4 % (517 of 1046)39.2 % (305 of 779)74.4 % (90 of 121)
Backbone38.1 % (426 of 1119)33.7 % (170 of 504)32.7 % (174 of 532)98.8 % (82 of 83)
Sidechain62.2 % (566 of 910)64.0 % (347 of 542)63.9 % (211 of 330)21.1 % (8 of 38)
Aromatic25.0 % (94 of 376)25.0 % (47 of 188)29.3 % (46 of 157) 3.2 % (1 of 31)
Methyl100.0 % (56 of 56)100.0 % (28 of 28)100.0 % (28 of 28)

1. 5S rRNA 55mer

GGGCCACACC UCUUGGGCCU GGUUAGUACC UGUUCGCUGG GAAUACCAGG UGCCC

2. TFIIIA zf46

MYVCHFENCG KAFKKHNQLK VHQFSHTQQL PYECPHEGCD KRFSLPSRLK RHEKVHAGYP CKKDDSCSFV GKTWTLYLKH VAECHQD

Sample

Temperature 291 (±0.1) K, pH 6.5 (±0.1)


#NameIsotope labelingTypeConcentration
15S rRNA 55mer0.6 ~ 2.0 mM
2TFIIIA zf46[U-15N]0.6 ~ 2.0 mM
3ZINC (II) ION1.8 ~ 6.0 mM
4KH2PO410 mM
5DTT20 mM

LACS Plot; CA
Referencing offset: -0.17 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: -0.17 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: 0.09 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2HGH, Strand ID: A Detail


RDC
98 RDC values in 1 lists
Field strength (1H) 900 MHz, Temperature 291 (±0.1) K, pH 6.5 (±0.1) Detail
Release date
2006-08-11
Citation
Induced fit and "lock and key" recognition of 5S RNA by zinc fingers of transcription factor IIIA
Lee, B.M., Xu, J., Clarkson, B.K., Martinez-Yamout, M.A., Dyson, H.JANE., Case, D.A., Gottesfeld, J.M., Wright, P.E.
J. Mol. Biol. (2006), 357, 275-291, PubMed 16405997 , DOI 10.1016/j.jmb.2005.12.010 ,
Related entities 1. 5S rRNA 55mer, : 1 : 1 entities Detail
Related entities 2. TFIIIA zf46, : 1 : 1 : 2 : 1 : 225 entities Detail
Experiments performed 24 experiments Detail
nullKeywords zinc finger