BMRBj - BMREntryMaster

Found 1 Document (1.621 seconds)

BMRB: 7209

2IVW

Assignment of 1H, 13C, and 15N resonances for the PilP pilot protein from Neisseria meningitidis.

Authors

Golovanov, A.P., Balasingham, S., Tzitzilonis, C., Goult, B.T., Lian, L., Homberset, H., Tonjum, T., Derrick, J.P.

Assembly

PilP

Entity

1. PilP (polymer, Thiol state: not present), 113 monomers, 12461.60 Da Detail

ETDKKGENAP DTKRIKETLE KFSLENMRYV GILKSGQKVS GFIEAEGYVY TVGVGNYLGQ NYGRIESITD DSIVLNELIE DSTGNWVSRK AELLLNSSDK NTEQAAAPAA EQN

Formula weight

12461.6 Da

Source organism

Neisseria meningitidis

Exptl. method

NMR

Refine. method

CYANA2.1

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 91.7 %, Completeness (bb): 97.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.7 % (1178 of 1284)	93.1 % (622 of 668)	89.6 % (440 of 491)	92.8 % (116 of 125)
Backbone	97.9 % (660 of 674)	97.4 % (228 of 234)	97.9 % (322 of 329)	99.1 % (110 of 111)
Sidechain	87.0 % (620 of 713)	90.8 % (394 of 434)	83.0 % (220 of 265)	42.9 % (6 of 14)
Aromatic	50.0 % (36 of 72)	97.2 % (35 of 36)	0.0 % (0 of 35)	100.0 % (1 of 1)
Methyl	97.5 % (119 of 122)	100.0 % (61 of 61)	95.1 % (58 of 61)

1. PilP

ETDKKGENAP DTKRIKETLE KFSLENMRYV GILKSGQKVS GFIEAEGYVY TVGVGNYLGQ NYGRIESITD DSIVLNELIE DSTGNWVSRK AELLLNSSDK NTEQAAAPAA EQN

Show sample condition

Sample #1

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Isotope labeling	Type	Concentration
1	PilP	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
2	phosphate buffer		buffer	20 mM
3	EDTA		chelating agent	1 mM
4	H2O		solvent	95 %
5	D2O		solvent	5 %

Sample #2

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Type	Concentration
6	PilP	protein	0.5 ~ 1.0 mM
7	phosphate buffer	buffer	20 mM
8	EDTA	chelating agent	1 mM
9	H2O	solvent	95 %
10	D2O	solvent	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 6 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.47 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2IVW, Strand ID: A Detail

Release date

2006-10-01

Citation

Assignment of 1H, 13C, and 15N resonances for the PilP pilot protein from Neisseria meningitidis
Golovanov, A.P., Balasingham, S., Tzitzilonis, C., Goult, B.T., Lian, L., Homberset, H., Tonjum, T., Derrick, J.P.
J. Biomol. NMR (2006), 36 Suppl 1, 68-68, PubMed 16933020 , DOI 10.1007/s10858-006-9054-5 , Abstract

Related entities 1. PilP, : 1 : 1 : 1 entities Detail

Experiments performed 11 experiments Detail

1 HNCO

Instrument

Model Bruker AVANCE DRX (600 MHz)

Sample #1

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Isotope labeling	Type	Concentration
1	PilP	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
2	phosphate buffer		buffer	20 mM
3	EDTA		chelating agent	1 mM
4	H2O		solvent	95 %
5	D2O		solvent	5 %

Sample #2

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Type	Concentration
6	PilP	protein	0.5 ~ 1.0 mM
7	phosphate buffer	buffer	20 mM
8	EDTA	chelating agent	1 mM
9	H2O	solvent	95 %
10	D2O	solvent	5 %

2 HNCACO

Instrument

Model Bruker AVANCE DRX (600 MHz)

Sample #1

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Isotope labeling	Type	Concentration
1	PilP	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
2	phosphate buffer		buffer	20 mM
3	EDTA		chelating agent	1 mM
4	H2O		solvent	95 %
5	D2O		solvent	5 %

Sample #2

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Type	Concentration
6	PilP	protein	0.5 ~ 1.0 mM
7	phosphate buffer	buffer	20 mM
8	EDTA	chelating agent	1 mM
9	H2O	solvent	95 %
10	D2O	solvent	5 %

3 HNCA

Instrument

Model Bruker AVANCE DRX (600 MHz)

Sample #1

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Isotope labeling	Type	Concentration
1	PilP	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
2	phosphate buffer		buffer	20 mM
3	EDTA		chelating agent	1 mM
4	H2O		solvent	95 %
5	D2O		solvent	5 %

Sample #2

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Type	Concentration
6	PilP	protein	0.5 ~ 1.0 mM
7	phosphate buffer	buffer	20 mM
8	EDTA	chelating agent	1 mM
9	H2O	solvent	95 %
10	D2O	solvent	5 %

4 HNCOCA

Instrument

Model Bruker AVANCE DRX (600 MHz)

Sample #1

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Isotope labeling	Type	Concentration
1	PilP	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
2	phosphate buffer		buffer	20 mM
3	EDTA		chelating agent	1 mM
4	H2O		solvent	95 %
5	D2O		solvent	5 %

Sample #2

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Type	Concentration
6	PilP	protein	0.5 ~ 1.0 mM
7	phosphate buffer	buffer	20 mM
8	EDTA	chelating agent	1 mM
9	H2O	solvent	95 %
10	D2O	solvent	5 %

5 HBHACONH

Instrument

Model Bruker AVANCE DRX (600 MHz)

Sample #1

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Isotope labeling	Type	Concentration
1	PilP	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
2	phosphate buffer		buffer	20 mM
3	EDTA		chelating agent	1 mM
4	H2O		solvent	95 %
5	D2O		solvent	5 %

Sample #2

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Type	Concentration
6	PilP	protein	0.5 ~ 1.0 mM
7	phosphate buffer	buffer	20 mM
8	EDTA	chelating agent	1 mM
9	H2O	solvent	95 %
10	D2O	solvent	5 %

6 CBCACONH

Instrument

Model Bruker AVANCE DRX (600 MHz)

Sample #1

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Isotope labeling	Type	Concentration
1	PilP	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
2	phosphate buffer		buffer	20 mM
3	EDTA		chelating agent	1 mM
4	H2O		solvent	95 %
5	D2O		solvent	5 %

Sample #2

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Type	Concentration
6	PilP	protein	0.5 ~ 1.0 mM
7	phosphate buffer	buffer	20 mM
8	EDTA	chelating agent	1 mM
9	H2O	solvent	95 %
10	D2O	solvent	5 %

7 HCCH-TOCSY

Instrument

Model Bruker AVANCE DRX (600 MHz)

Sample #1

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Isotope labeling	Type	Concentration
1	PilP	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
2	phosphate buffer		buffer	20 mM
3	EDTA		chelating agent	1 mM
4	H2O		solvent	95 %
5	D2O		solvent	5 %

Sample #2

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Type	Concentration
6	PilP	protein	0.5 ~ 1.0 mM
7	phosphate buffer	buffer	20 mM
8	EDTA	chelating agent	1 mM
9	H2O	solvent	95 %
10	D2O	solvent	5 %

8 NOESY-HSQC

Instrument

Model Bruker AVANCE DRX (600 MHz)

Sample #1

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Isotope labeling	Type	Concentration
1	PilP	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
2	phosphate buffer		buffer	20 mM
3	EDTA		chelating agent	1 mM
4	H2O		solvent	95 %
5	D2O		solvent	5 %

Sample #2

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Type	Concentration
6	PilP	protein	0.5 ~ 1.0 mM
7	phosphate buffer	buffer	20 mM
8	EDTA	chelating agent	1 mM
9	H2O	solvent	95 %
10	D2O	solvent	5 %

9 TOCSY-HSQC

Instrument

Model Bruker AVANCE DRX (600 MHz)

Sample #1

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Isotope labeling	Type	Concentration
1	PilP	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
2	phosphate buffer		buffer	20 mM
3	EDTA		chelating agent	1 mM
4	H2O		solvent	95 %
5	D2O		solvent	5 %

Sample #2

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Type	Concentration
6	PilP	protein	0.5 ~ 1.0 mM
7	phosphate buffer	buffer	20 mM
8	EDTA	chelating agent	1 mM
9	H2O	solvent	95 %
10	D2O	solvent	5 %

10 NOESY

Instrument

Model Bruker AVANCE DRX (600 MHz)

Sample #1

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Isotope labeling	Type	Concentration
1	PilP	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
2	phosphate buffer		buffer	20 mM
3	EDTA		chelating agent	1 mM
4	H2O		solvent	95 %
5	D2O		solvent	5 %

Sample #2

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Type	Concentration
6	PilP	protein	0.5 ~ 1.0 mM
7	phosphate buffer	buffer	20 mM
8	EDTA	chelating agent	1 mM
9	H2O	solvent	95 %
10	D2O	solvent	5 %

11 TOCSY

Instrument

Model Bruker AVANCE DRX (600 MHz)

Sample #1

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Isotope labeling	Type	Concentration
1	PilP	[U-13C; U-15N]	protein	0.5 ~ 1.0 mM
2	phosphate buffer		buffer	20 mM
3	EDTA		chelating agent	1 mM
4	H2O		solvent	95 %
5	D2O		solvent	5 %

Sample #2

Temperature 303 (±0.1) K, pH 6.1 (±0.1), Details PilP fragment, residues 69-181

#	Name	Type	Concentration
6	PilP	protein	0.5 ~ 1.0 mM
7	phosphate buffer	buffer	20 mM
8	EDTA	chelating agent	1 mM
9	H2O	solvent	95 %
10	D2O	solvent	5 %

nullKeywords lipoprotein, pilot protein, pilus biogenesis, secretin, Neisseria meningitidis

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.621 seconds)

BMRB: 7209

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. PilP, : 1 : 1 : 1 entities Detail

Experiments performed 11 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2