BMRB: 7220

ephrinB2 ectodomain

Authors

Ran, X., Fan, J., Song, J.

Assembly

ephrinB2 ectodomain monomer

Entity

1. ephrinB2 ectodomain monomer (polymer, Thiol state: all disulfide bound), 151 monomers, 17019.20 Da Detail

SKSIVLEPIY WNSSNSKFLP GQGLVLYPQI GDKLDIICPK VDSKTVGQYE YYKVYMVDKD QADRCTIKKE NTPLLNCAKP DQDIKFTIKF QEFSPNLWGL EFQKNKDYYI ISTSNGSLEG LDNQEGGVCQ TRAMKILMKV GQDASSAGST R

Formula weight

17019.2 Da

Entity Connection

disulfide 2 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS38:SG	1:CYS77:SG
2	disulfide	sing	1:CYS65:SG	1:CYS129:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.0 %, Completeness: 80.1 %, Completeness (bb): 95.7 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	80.1 % (1442 of 1801)	74.8 % (707 of 945)	83.8 % (580 of 692)	94.5 % (155 of 164)
Backbone	95.7 % (854 of 892)	94.8 % (290 of 306)	95.7 % (423 of 442)	97.9 % (141 of 144)
Sidechain	69.0 % (724 of 1049)	65.3 % (417 of 639)	75.1 % (293 of 390)	70.0 % (14 of 20)
Aromatic	15.9 % (22 of 138)	18.8 % (13 of 69)	13.4 % (9 of 67)	0.0 % (0 of 2)
Methyl	86.5 % (128 of 148)	87.8 % (65 of 74)	85.1 % (63 of 74)

1. ephrinB2 ectodomain

SKSIVLEPIY WNSSNSKFLP GQGLVLYPQI GDKLDIICPK VDSKTVGQYE YYKVYMVDKD QADRCTIKKE NTPLLNCAKP DQDIKFTIKF QEFSPNLWGL EFQKNKDYYI ISTSNGSLEG LDNQEGGVCQ TRAMKILMKV GQDASSAGST R

Show sample condition

Sample

Temperature 293 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	ephrinB2 ectodomain	[U-13C; U-15N]		0.5 mM
2	phosphate buffer			20 mM

Hide sample codition

LACS Plot; CA

Referencing offset: -0.42 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.42 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 3.13 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 10 models in PDB: 2I85, Strand ID: A Detail

---

Release date

2006-11-05

Citation

NMR assignment of the human ephrinB2 ectodomain
Ran, X., Fan, J., Song, J.
J. Biomol. NMR (2007), 38, 171-171, PubMed 17061024 , DOI 10.1007/s10858-006-9088-8 , Abstract

Related entities 1. ephrinB2 ectodomain monomer, : 1 : 2 : 1 : 5 : 39 entities Detail

Interaction partners 1. ephrinB2 ectodomain monomer, : 9 interactors Detail

Experiments performed 8 experiments Detail