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Found 1 Document (1.93 seconds)

BMRB: 7223

2HJ8

Solution NMR structure of the C-terminal domain of the interferon alpha-inducible ISG15 protein from Homo sapiens. Northeast Structural Genomics target HR2873B

Authors

Aramini, J.M., Ho, C.K., Yin, C., Cunningham, K., Janjua, H., Ma, L., Xiao, R., Acton, T.B., Montelione, G.T.

Assembly

Interferon, alpha-inducible protein

Entity

1. Interferon, alpha-inducible protein (polymer, Thiol state: not present), 88 monomers, 10097.34 Da Detail

MDEPLSILVR NNKGRSSTYE VRLTQTVAHL KQQVSGLEGV QDDLFWLTFE GKPLEDQLPL GEYGLKPLST VFMNLRLRGG LEHHHHHH

Formula weight

10097.34 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 94.3 %, Completeness: 91.8 %, Completeness (bb): 92.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.8 % (963 of 1049)	91.6 % (501 of 547)	91.9 % (376 of 409)	92.5 % (86 of 93)
Backbone	92.5 % (481 of 520)	92.8 % (167 of 180)	92.6 % (237 of 256)	91.7 % (77 of 84)
Sidechain	91.3 % (556 of 609)	91.0 % (334 of 367)	91.4 % (213 of 233)	100.0 % (9 of 9)
Aromatic	67.4 % (58 of 86)	67.4 % (29 of 43)	66.7 % (28 of 42)	100.0 % (1 of 1)
Methyl	100.0 % (100 of 100)	100.0 % (50 of 50)	100.0 % (50 of 50)

1. Interferon-induced 17 kDa protein

MDEPLSILVR NNKGRSSTYE VRLTQTVAHL KQQVSGLEGV QDDLFWLTFE GKPLEDQLPL GEYGLKPLST VFMNLRLRGG LEHHHHHH

Show sample condition

Sample

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Interferon-induced 17 kDa protein	[U-13C; U-15N]	0.81 mM
2	ammonium citrate		50 mM
3	CaCl2		5 mM
4	NaN3		0.02 %
5	D2O		5 %
6	H2O		95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.17 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2HJ8, Strand ID: A Detail

Coupling constant

57 J values in 1 lists
Pressure 1 atm, Temperature 293 K, pH 6.5 Detail

Release date

2009-05-27

Citation

Solution NMR structure of the C-terminal domain of the interferon alpha-inducible ISG15 protein from Homo sapiens. Northeast Structural Genomics target HR2873B
Aramini, J.M., Ho, C.K., Yin, C., Cunningham, K., Janjua, H., Li, M., Xiao, R., Acton, T.B., Montelione, G.T.

Related entities 1. Interferon, alpha-inducible protein, : 1 : 15 : 694 entities Detail

Interaction partners 1. Interferon, alpha-inducible protein, : 14 interactors Detail

More details for 14 interactors identified by 11 citations

Experiments performed 8 experiments Detail

1 3D 15N-separated NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Interferon-induced 17 kDa protein	[U-13C; U-15N]	0.81 mM
2	ammonium citrate		50 mM
3	CaCl2		5 mM
4	NaN3		0.02 %
5	D2O		5 %
6	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Interferon-induced 17 kDa protein	[U-5% 13C; U-15N]	0.9 mM
8	ammonium citrate		50 mM
9	CaCl2		5 mM
10	NaN3		0.02 %
11	D2O		5 %
12	H2O		95 %

2 3D 13C-separated NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Interferon-induced 17 kDa protein	[U-13C; U-15N]	0.81 mM
2	ammonium citrate		50 mM
3	CaCl2		5 mM
4	NaN3		0.02 %
5	D2O		5 %
6	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Interferon-induced 17 kDa protein	[U-5% 13C; U-15N]	0.9 mM
8	ammonium citrate		50 mM
9	CaCl2		5 mM
10	NaN3		0.02 %
11	D2O		5 %
12	H2O		95 %

3 HNHA

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Interferon-induced 17 kDa protein	[U-13C; U-15N]	0.81 mM
2	ammonium citrate		50 mM
3	CaCl2		5 mM
4	NaN3		0.02 %
5	D2O		5 %
6	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Interferon-induced 17 kDa protein	[U-5% 13C; U-15N]	0.9 mM
8	ammonium citrate		50 mM
9	CaCl2		5 mM
10	NaN3		0.02 %
11	D2O		5 %
12	H2O		95 %

4 high resolution 2D CH-HSQC (for stereospecific assignment of Val/Leu methyls)

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Interferon-induced 17 kDa protein	[U-13C; U-15N]	0.81 mM
2	ammonium citrate		50 mM
3	CaCl2		5 mM
4	NaN3		0.02 %
5	D2O		5 %
6	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Interferon-induced 17 kDa protein	[U-5% 13C; U-15N]	0.9 mM
8	ammonium citrate		50 mM
9	CaCl2		5 mM
10	NaN3		0.02 %
11	D2O		5 %
12	H2O		95 %

5 2D 15N 1H heteronuclear NOE

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Interferon-induced 17 kDa protein	[U-13C; U-15N]	0.81 mM
2	ammonium citrate		50 mM
3	CaCl2		5 mM
4	NaN3		0.02 %
5	D2O		5 %
6	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Interferon-induced 17 kDa protein	[U-5% 13C; U-15N]	0.9 mM
8	ammonium citrate		50 mM
9	CaCl2		5 mM
10	NaN3		0.02 %
11	D2O		5 %
12	H2O		95 %

6 3D TR backbone expts

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Interferon-induced 17 kDa protein	[U-13C; U-15N]	0.81 mM
2	ammonium citrate		50 mM
3	CaCl2		5 mM
4	NaN3		0.02 %
5	D2O		5 %
6	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Interferon-induced 17 kDa protein	[U-5% 13C; U-15N]	0.9 mM
8	ammonium citrate		50 mM
9	CaCl2		5 mM
10	NaN3		0.02 %
11	D2O		5 %
12	H2O		95 %

7 3D HCCH-COSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Interferon-induced 17 kDa protein	[U-13C; U-15N]	0.81 mM
2	ammonium citrate		50 mM
3	CaCl2		5 mM
4	NaN3		0.02 %
5	D2O		5 %
6	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Interferon-induced 17 kDa protein	[U-5% 13C; U-15N]	0.9 mM
8	ammonium citrate		50 mM
9	CaCl2		5 mM
10	NaN3		0.02 %
11	D2O		5 %
12	H2O		95 %

8 3D HCCH-TOCSYs

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Interferon-induced 17 kDa protein	[U-13C; U-15N]	0.81 mM
2	ammonium citrate		50 mM
3	CaCl2		5 mM
4	NaN3		0.02 %
5	D2O		5 %
6	H2O		95 %

Sample #2

Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	Interferon-induced 17 kDa protein	[U-5% 13C; U-15N]	0.9 mM
8	ammonium citrate		50 mM
9	CaCl2		5 mM
10	NaN3		0.02 %
11	D2O		5 %
12	H2O		95 %

nullKeywords HR2873B, human ISG15, NESG, NMR structure, Northeast Structural Genomics Consortium, Protein Structure Initiative, PSI-1

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Found 1 Document (1.93 seconds)

BMRB: 7223

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Interferon, alpha-inducible protein, : 1 : 15 : 694 entities Detail

Interaction partners 1. Interferon, alpha-inducible protein, : 14 interactors Detail

Experiments performed 8 experiments Detail

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