BMRB: 7227

Solution nmr structure of hypothetical protein yppE: Northeast Structural Genomics Consortium Target SR213

Authors

Liu, G., Singarapu, K.K., Parish, D., Eletsky, A., Xu, D., Sukumaran, D., Ho, C.K., Fang, Y., Cunningham, K., Ma, L., Xiao, R., Liu, J., Baran, M., Swapna, G.V.T., Acton, T.B., Rost, B., Montelione, G.T., Szyperski, T.

Assembly

Hypothetical protein yppE

Entity

1. Hypothetical protein yppE (polymer, Thiol state: not present), 131 monomers, 15524.27 Da Detail

MLSQTLLEMT EQMIEVAEKG ADRYQEGKNS NHSYDFFETI KPAVEENDEL AARWAEGALE LIKVRRPKYV HKEQIEAVKD NFLELVLQSY VHHIHKKRFK DITESVLYTL HAVKDEIARE DSRLEHHHHH H

Formula weight

15524.27 Da

Source organism

Bacillus subtilis

Exptl. method

NMR

Refine. method

simulated annealing,

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.0 %, Completeness: 75.0 %, Completeness (bb): 70.1 % Detail

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• NMR-STAR v3.1 (partial)
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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	75.0 % (1189 of 1585)	81.5 % (674 of 827)	66.6 % (412 of 619)	74.1 % (103 of 139)
Backbone	70.1 % (548 of 782)	80.2 % (211 of 263)	61.5 % (240 of 390)	75.2 % (97 of 129)
Sidechain	80.3 % (748 of 931)	82.1 % (463 of 564)	78.2 % (279 of 357)	60.0 % (6 of 10)
Aromatic	58.6 % (82 of 140)	71.4 % (50 of 70)	44.9 % (31 of 69)	100.0 % (1 of 1)
Methyl	95.8 % (136 of 142)	95.8 % (68 of 71)	95.8 % (68 of 71)

1. Hypothetical protein yppE

MLSQTLLEMT EQMIEVAEKG ADRYQEGKNS NHSYDFFETI KPAVEENDEL AARWAEGALE LIKVRRPKYV HKEQIEAVKD NFLELVLQSY VHHIHKKRFK DITESVLYTL HAVKDEIARE DSRLEHHHHH H

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Hypothetical protein yppE			0.94 mM
2	Arginine			50 mM
3	DTT			10 mM
4	Bis Tris			50 mM
5	H2O			95 %
6	D2O			5 %

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LACS Plot; CA

Referencing offset: -0.31 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.31 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2HFI, Strand ID: A Detail

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Release date

2010-03-03

Citation

Solution NMR structure of hypothetical protein yppE: Northeast Structural Genomics Consortium Target SR213
Liu, G., Singarapu, K.K., Parish, D., Eletsky, A., Sukumaran, D.K., Xu, D., Ho, C.K., Fang, Y., Cunningham, K., Ma, L., Xiao, R., Liu, J., Baran, M., Swapna, G.V.T., Acton, T.B., Rost, B., Montelione, G.T., Szyperski, T.

Related entities 1. Hypothetical protein yppE, : 1 : 2 : 3 entities Detail

Experiments performed 6 experiments Detail