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BMRB: 7229


7229
2HDL
Solution structure of Brak/CXCL14
Authors
Volkman, B.F., Peterson, F.C., Schwarze, S.R., Harder, A.G.
Assembly
Small inducible cytokine B14
Entity
1. Small inducible cytokine B14 (polymer, Thiol state: all disulfide bound), 78 monomers, 9476.004 Da Detail

GSKCKCSRKG PKIRYSDVKK LEMKPKYPHC EEKMVIITTK SVSRYRGQEH CLHPKLQSTK RFIKWYNAWN EKRRVYEE


Formula weight
9476.004 Da
Entity Connection
disulfide 2 Detail
IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing1:CYS4:SG1:CYS30:SG
2disulfidesing1:CYS6:SG1:CYS51:SG

Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
AUTOMATED METHODS WERE USED FOR BACKBONE CHEMICAL SHIFT ASSIGNMENT AND ITERATIVE NOE REFINEMENT.
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.4 %, Completeness: 87.0 %, Completeness (bb): 87.8 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All87.0 % (870 of 1000)87.6 % (472 of 539)85.8 % (327 of 381)88.7 % (71 of 80)
Backbone87.8 % (404 of 460)87.1 % (135 of 155)88.3 % (204 of 231)87.8 % (65 of 74)
Sidechain86.3 % (531 of 615)87.8 % (337 of 384)83.6 % (188 of 225)100.0 % (6 of 6)
Aromatic81.4 % (70 of 86)95.3 % (41 of 43)65.9 % (27 of 41)100.0 % (2 of 2)
Methyl100.0 % (52 of 52)100.0 % (26 of 26)100.0 % (26 of 26)

1. Small inducible cytokine B14

GSKCKCSRKG PKIRYSDVKK LEMKPKYPHC EEKMVIITTK SVSRYRGQEH CLHPKLQSTK RFIKWYNAWN EKRRVYEE

Show sample condition
Sample

Pressure 1 atm, Temperature 298 K, pH 6.5


#NameIsotope labelingTypeConcentration
1Small inducible cytokine B14[U-15N; U-13C]1 mM
2phosphate buffer100 mM
3NaCl150 mM
4H2O95 %
5D2O5 %

LACS Plot; CA
Referencing offset: -0.43 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: -0.43 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: 0.01 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: -0.04 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2HDL, Strand ID: A Detail

Release date
2006-10-18
Citation
Structural determinants involved in the regulation of CXCL14/BRAK expression by the 26 S proteasome
Peterson, F.C., Thorpe, J.A., Harder, A.G., Volkman, B.F., Schwarze, S.R.
J. Mol. Biol. (2006), 363, 813-822, PubMed 16987528 , DOI 10.1016/j.jmb.2006.08.057 ,
Entries sharing articles Swiss-Prot: 1 entries Detail
  Swiss-Prot: O95715 released on 2000-05-30
    Title CXL14_HUMAN Entity C-X-C motif chemokine 14
Related entities 1. Small inducible cytokine B14, : 1 : 2 : 3 : 34 entities Detail
More details for 40 related entities
Interaction partners 1. Small inducible cytokine B14, : 27 interactors Detail
More details for 27 interactors identified by 8 citations
Experiments performed 3 experiments Detail
nullKeywords Brak, chemokine, CXCL14, NMR