BMRB: 7230

The NMR Structure of an Internal Loop from 23S Ribosomal RNA Differs from its Structure in Crystals of the 50S Ribosomal Subunit

Authors

Shankar, N.

Assembly

5'-R(*GP*GP*CP*UP*AP*AP*GP*AP*C)-3', 5'-R(*GP*UP*CP*GP*AP*AP*GP*CP*C)-3'

Entity

1. 5'-R(*GP*GP*CP*UP*AP*AP*GP*AP*C)-3' (polymer, Thiol state: not present), 9 monomers, 2957.779 Da Detail

GGCUAAGAC

2. 5'-R(*GP*UP*CP*GP*AP*AP*GP*CP*C)-3' (polymer, Thiol state: not present), 9 monomers, 2933.755 Da Detail

GUCGAAGCC

Total weight

5891.534 Da

Max. entity weight

2957.779 Da

Source organism

Deinococcus radiodurans

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 50.0 %, Completeness: 33.3 %, Completeness (bb): 28.7 % Detail

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Polymer type: polyribonucleotide

	Total	1H
All	33.3 % (52 of 156)	33.3 % (52 of 156)
Suger, PO4	28.7 % (31 of 108)	28.7 % (31 of 108)
Nucleobase	43.8 % (21 of 48)	43.8 % (21 of 48)
Aromatic	50.0 % (19 of 38)	50.0 % (19 of 38)

1. 5'-R(*GP*GP*CP*UP*AP*AP*GP*AP*C)-3'

GGCUAAGAC

2. 5'-R(*GP*UP*CP*GP*AP*AP*GP*CP*C)-3'

GUCGAAGCC

Show sample condition

Sample

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	5'-R(*GP*GP*CP*UP*AP*AP*GP*AP*C)-3'			2 mM
2	5'-R(*GP*UP*CP*GP*AP*AP*GP*CP*C)-3'			2 mM
3	NaCl			80 mM
4	SODIUM PHOSPHATE			10 mM
5	Na2EDTA			0.5 mM
6	H2O			90 %
7	D2O			10 %

Hide sample codition

Chem. Shift Complete2

Sequence coverage: 50.0 %, Completeness: 32.7 %, Completeness (bb): 27.3 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
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Polymer type: polyribonucleotide

	Total	1H
All	32.7 % (102 of 312)	32.7 % (102 of 312)
Suger, PO4	27.3 % (59 of 216)	27.3 % (59 of 216)
Nucleobase	44.8 % (43 of 96)	44.8 % (43 of 96)
Aromatic	48.7 % (37 of 76)	48.7 % (37 of 76)

1. 5'-R(*GP*GP*CP*UP*AP*AP*GP*AP*C)-3'

GGCUAAGAC

2. 5'-R(*GP*UP*CP*GP*AP*AP*GP*CP*C)-3'

GUCGAAGCC

Show sample condition

Sample

Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	5'-R(*GP*GP*CP*UP*AP*AP*GP*AP*C)-3'			2 mM
2	5'-R(*GP*UP*CP*GP*AP*AP*GP*CP*C)-3'			2 mM
3	NaCl			80 mM
4	SODIUM PHOSPHATE			10 mM
5	Na2EDTA			0.5 mM
6	H2O			90 %
7	D2O			10 %

Hide sample codition

Release date

2006-10-18

Citation

The NMR structure of an internal loop from 23S ribosomal RNA differs from its structure in crystals of 50s ribosomal subunits
Shankar, N., Kennedy, S.D., Chen, G., Krugh, T.R., Turner, D.H.
Biochemistry (2006), 45, 11776-11789, PubMed 17002278 , DOI 10.1021/bi0605787 , Abstract

Experiments performed 1 experiments Detail