BMRB: 7241

Chromo 2 domain of cpSRP43 complexed with cpSRP54 peptide

Authors

Kathir, K.MUTHUSAMY., Vaithiyalingam, S., Dakshinamurthy, R., Henry, R., Thallapuranam, S.KRISHNASWAMY.

Assembly

Chromo domain 2 complexed with cpSRP54 peptide

Entity

1. CD2 of cpSRP43 (polymer, Thiol state: all free), 57 monomers, 6539.076 Da Detail

GSQVFEYAEV DEIVEKRGKG KDVEYLVRWK DGGDCEWVKG VHVAEDVAKD YEDGLEY

2. cpSRP54 peptide (polymer, Thiol state: all free), 14 monomers, 1510.700 Da Detail

APPGTARRKR KADS

Total weight

8049.7764 Da

Max. entity weight

6539.076 Da

Source organism

Arabidopsis thaliana

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing, molecular dynamics, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 80.3 %, Completeness: 57.7 %, Completeness (bb): 67.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	57.7 % (467 of 810)	60.6 % (257 of 424)	48.7 % (153 of 314)	79.2 % (57 of 72)
Backbone	67.3 % (284 of 422)	79.7 % (118 of 148)	53.2 % (109 of 205)	82.6 % (57 of 69)
Sidechain	51.7 % (233 of 451)	50.4 % (139 of 276)	54.7 % (94 of 172)	0.0 % (0 of 3)
Aromatic	0.0 % (0 of 70)	0.0 % (0 of 35)	0.0 % (0 of 33)	0.0 % (0 of 2)
Methyl	74.2 % (46 of 62)	77.4 % (24 of 31)	71.0 % (22 of 31)

1. CD2 of cpSRP43

GSQVFEYAEV DEIVEKRGKG KDVEYLVRWK DGGDCEWVKG VHVAEDVAKD YEDGLEY

2. cpSRP54 peptide

APPGTARRKR KADS

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	CD2 domain of cpSRP43	[U-13C; U-15N]	protein	1.0 (±0.05) mM
2	cpSRP54 peptide		protein	1.0 (±0.05) mM
3	Phosphate			20 mM
4	NaCl			50 mM
5	sodium azide			1 mM

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LACS Plot; CA

Referencing offset: 2.62 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
15N	TSP	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.62 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
15N	TSP	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.11 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
15N	TSP	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2HUG, Strand ID: A, B Detail

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Release date

2007-07-23

Citation

Structural Characterization of the Interface of the Subunits Chloroplast Signal Recognition Particle (cpSRP) ? Determination of the Three-Dimensional Solution Structure of the Chromo Domain 2 ? cpSRP54 Peptide Complex
Kathir, K.MUTHUSAMY., Vaithiyalingam, S., Dakshinamurthy, R., Henry, R., Thallapuranam, S.KRISHNASWAMY.

Related entities 1. CD2 of cpSRP43, : 2 : 21 entities Detail

Related entities 2. cpSRP54 peptide, : 1 : 2 : 1 entities Detail

Interaction partners 1. CD2 of cpSRP43, : 20 : 1 interactors Detail

Interaction partners 2. cpSRP54 peptide, : 4 : 1 interactors Detail

Experiments performed 1 experiments Detail