BMRB: 7243

beta-microseminoprotein

Authors

Ghasriani, H., Teilum, K., Johnsson, Y., Fernlund, P., Drakenberg, T.

Assembly

pMSP

Entity

1. pMSP (polymer, Thiol state: all disulfide bound), 94 monomers, 10265.60 Da Detail

GGGQCYFIPN QSLKPNECQD LKGVSHPLNS VWKTKDCEEC TCGQDAISCC NTAAIPTGYD TNKCQKILNK KTCTYTVVEK KDPGKTCDVT GWVL

Formula weight

10265.6 Da

Entity Connection

disulfide 5 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS5:SG	1:CYS50:SG
2	disulfide	sing	1:CYS18:SG	1:CYS42:SG
3	disulfide	sing	1:CYS37:SG	1:CYS73:SG
4	disulfide	sing	1:CYS40:SG	1:CYS49:SG
5	disulfide	sing	1:CYS64:SG	1:CYS87:SG

Source organism

Sus scrofa

Exptl. method

NMR

Refine. method

ANNEAL

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.9 %, Completeness: 87.5 %, Completeness (bb): 95.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	87.5 % (924 of 1056)	92.0 % (506 of 550)	79.0 % (319 of 404)	97.1 % (99 of 102)
Backbone	95.8 % (531 of 554)	96.9 % (185 of 191)	94.9 % (260 of 274)	96.6 % (86 of 89)
Sidechain	81.0 % (476 of 588)	89.4 % (321 of 359)	65.7 % (142 of 216)	100.0 % (13 of 13)
Aromatic	40.3 % (25 of 62)	74.2 % (23 of 31)	0.0 % (0 of 29)	100.0 % (2 of 2)
Methyl	93.0 % (80 of 86)	97.7 % (42 of 43)	88.4 % (38 of 43)

1. beta-microseminoprotein

GGGQCYFIPN QSLKPNECQD LKGVSHPLNS VWKTKDCEEC TCGQDAISCC NTAAIPTGYD TNKCQKILNK KTCTYTVVEK KDPGKTCDVT GWVL

Show sample condition

Sample

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	beta-microseminoprotein			4.0 (±0.2) mM
2	H2O			90 %
3	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.03 ppm, Outliers: 6 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	null	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	null	0.1013291

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LACS Plot; CB

Referencing offset: -0.03 ppm, Outliers: 6 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	null	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	null	0.1013291

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	null	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	null	0.1013291

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LACS Plot; CO

Referencing offset: 0.32 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	null	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	null	0.1013291

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Protein Blocks Logo

Calculated from 10 models in PDB: 2IZ4, Strand ID: A Detail

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Release date

2006-10-19

Citation

Solution structures of human and porcine beta-microseminoprotein
Ghasriani, H., Teilum, K., Johnsson, Y., Fernlund, P., Drakenberg, T.
J. Mol. Biol. (2006), 362, 502-515, PubMed 16930619 , DOI 10.1016/j.jmb.2006.07.029 , Abstract

Related entities 1. pMSP, : 1 : 1 : 20 entities Detail

Experiments performed 5 experiments Detail