BMRB: 7259

The solution structure of the BRCT domain from human polymerase reveals homology with the TdT BRCT domain

Authors

DeRose, E.F., Clarkson, M.W., Gilmore, S.A., Ramsden, D.A., Mueller, G.A., London, R.E., Lee, A.L.

Assembly

DNA polymerase mu (E.C.2.7.7.7)

Entity

1. DNA polymerase mu (E.C.2.7.7.7) (polymer, Thiol state: all free), 105 monomers, 11405.91 Da Detail

GTPPSTRFPG VAIYLVEPRM GRSRRAFLTG LARSKGFRVL DACSSEATHV VMEETSAEEA VSWQERRMAA APPGCTPPAL LDISWLTESL GAGQPVPVEC RHRLE

Formula weight

11405.91 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Torsion angle dynamics followed by molecular dynamics refinement in explicit water solvent

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 90.9 %, Completeness (bb): 92.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.9 % (1056 of 1162)	90.1 % (544 of 604)	90.8 % (417 of 459)	96.0 % (95 of 99)
Backbone	92.8 % (566 of 610)	92.8 % (193 of 208)	91.9 % (282 of 307)	95.8 % (91 of 95)
Sidechain	89.7 % (582 of 649)	88.6 % (351 of 396)	91.2 % (227 of 249)	100.0 % (4 of 4)
Aromatic	80.0 % (56 of 70)	80.0 % (28 of 35)	78.8 % (26 of 33)	100.0 % (2 of 2)
Methyl	100.0 % (114 of 114)	100.0 % (57 of 57)	100.0 % (57 of 57)

1. DNA polymerase mu (E.C.2.7.7.7)

GTPPSTRFPG VAIYLVEPRM GRSRRAFLTG LARSKGFRVL DACSSEATHV VMEETSAEEA VSWQERRMAA APPGCTPPAL LDISWLTESL GAGQPVPVEC RHRLE

Show sample condition

Sample

Pressure 1 atm, Temperature 283 K, pH 7.9

#	Name	Isotope labeling	Type	Concentration
1	DNA polymerase mu (E.C.2.7.7.7)	[U-13C; U-15N]		1 mM
2	d-Tris			25 mM
3	KCl			50 mM
4	NaN3			0.02 %
5	DTT			5 mM
6	H2O			90 %
7	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.34 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 11 models in PDB: 2HTF, Strand ID: A Detail

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Release date

2007-11-20

Citation

Solution structure of polymerase mu's BRCT Domain reveals an element essential for its role in nonhomologous end joining
DeRose, E.F., Clarkson, M.W., Gilmore, S.A., Galban, C.J., Tripathy, A., Havener, J.M., Mueller, G.A., Ramsden, D.A., London, R.E., Lee, A.L.
Biochemistry (2007), 46, 12100-12110, PubMed 17915942 , DOI 10.1021/bi7007728 , Abstract

Related entities 1. DNA polymerase mu (E.C.2.7.7.7), : 1 : 2 : 19 entities Detail

Interaction partners 1. DNA polymerase mu (E.C.2.7.7.7), : 3 interactors Detail

Experiments performed 2 experiments Detail