1H, 13C, and 15N Chemical Shift Assignments for the N-terminal Extracellular Domain of Truncated Cadherin
SIVVSPILIP ENQRQPFPRD VGKVVDSDRP ERSKFRLTGK GVDQEPKGIF RINENTGSVS VTRTLDREVI AVYQLFVETT DVNGKTLEGP VPLEVIVIDQ NDNRP
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 93.2 % (1142 of 1225) | 91.8 % (590 of 643) | 94.7 % (450 of 475) | 95.3 % (102 of 107) |
Backbone | 96.7 % (592 of 612) | 98.6 % (205 of 208) | 95.5 % (294 of 308) | 96.9 % (93 of 96) |
Sidechain | 91.1 % (648 of 711) | 88.5 % (385 of 435) | 95.8 % (254 of 265) | 81.8 % (9 of 11) |
Aromatic | 54.2 % (26 of 48) | 54.2 % (13 of 24) | 54.2 % (13 of 24) | |
Methyl | 100.0 % (132 of 132) | 100.0 % (66 of 66) | 100.0 % (66 of 66) |
1. N-terminal extracellular domain of truncated cadherin
SIVVSPILIP ENQRQPFPRD VGKVVDSDRP ERSKFRLTGK GVDQEPKGIF RINENTGSVS VTRTLDREVI AVYQLFVETT DVNGKTLEGP VPLEVIVIDQ NDNRPTemperature 298 (±0.1) K, pH 7.8 (±0.02)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | N-terminal extracellular domain of truncated cadherin | [U-98% 13C; U-99% 15N] | protein | 0.6 mM |
2 | TRIS | buffer | 5.0 mM | |
3 | NaCl | salt | 10.0 mM | |
4 | EDTA | chelating agent | 1.0 mM | |
5 | TCEP | reducing agent | 1.0 mM |