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BMRB: 7268

2V37

1H, 13C, and 15N Chemical Shift Assignments for the N-terminal Extracellular Domain of Truncated Cadherin

Authors

Bang, E., Haussinger, D., Stetefeld, J., Ahrens, T., Dames, S.A., Grzesiek, S.

Assembly

TCAD1 monomer

Entity

1. TCAD1 monomer (polymer, Thiol state: not present), 105 monomers, 11741.12 Da Detail

SIVVSPILIP ENQRQPFPRD VGKVVDSDRP ERSKFRLTGK GVDQEPKGIF RINENTGSVS VTRTLDREVI AVYQLFVETT DVNGKTLEGP VPLEVIVIDQ NDNRP

Formula weight

11741.12 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

X-PLOR-NIH

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.2 %, Completeness (bb): 96.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.2 % (1142 of 1225)	91.8 % (590 of 643)	94.7 % (450 of 475)	95.3 % (102 of 107)
Backbone	96.7 % (592 of 612)	98.6 % (205 of 208)	95.5 % (294 of 308)	96.9 % (93 of 96)
Sidechain	91.1 % (648 of 711)	88.5 % (385 of 435)	95.8 % (254 of 265)	81.8 % (9 of 11)
Aromatic	54.2 % (26 of 48)	54.2 % (13 of 24)	54.2 % (13 of 24)
Methyl	100.0 % (132 of 132)	100.0 % (66 of 66)	100.0 % (66 of 66)

1. N-terminal extracellular domain of truncated cadherin

SIVVSPILIP ENQRQPFPRD VGKVVDSDRP ERSKFRLTGK GVDQEPKGIF RINENTGSVS VTRTLDREVI AVYQLFVETT DVNGKTLEGP VPLEVIVIDQ NDNRP

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 7.8 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal extracellular domain of truncated cadherin	[U-98% 13C; U-99% 15N]	protein	0.6 mM
2	TRIS		buffer	5.0 mM
3	NaCl		salt	10.0 mM
4	EDTA		chelating agent	1.0 mM
5	TCEP		reducing agent	1.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.81 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2V37, Strand ID: A Detail

Release date

2007-01-08

Citation

1H, 13C, and 15N chemical shift assignments for the N-terminal extracellular domain of T-cadherin
Bang, E., Haussinger, D., Stetefeld, J., Ahrens, T., Grzesiek, S., Dames, S.A.
J. Biomol. NMR (2007), 38, 179-179, PubMed 17180550 , DOI 10.1007/s10858-006-9106-x , Abstract

Related entities 1. TCAD1 monomer, : 1 : 3 : 312 entities Detail

Interaction partners 1. TCAD1 monomer, : 10 interactors Detail

More details for 10 interactors identified by 4 citations

Experiments performed 9 experiments Detail

1 3D HNCA