BMRB: 7269

1H, 13C, 15N sequence-specific backbone and sidechain resonance assignments for dockerin-containing C-terminal region of the NagH hyaluronidase from Clostridium perfringens

Authors

Chitayat, S., Adams, J.J., Smith, S.P.

Assembly

fivar doc

Entity

1. fivar doc (polymer, Thiol state: not present), 140 monomers, 15489.79 Da Detail

MDKTNLGELI NQGKSLLDES VEGFNVGEYH KGAKDGLTVE INKAEEVFNK EDATEEEINL AKESLEGAIA RFNSLLIEES TGDFNGNGKI DIGDLAMVSK NIGSTTNTSL DLNKDGSIDE YEISFINHRI LNLEHHHHHH

Formula weight

15489.79 Da

Source organism

Clostridium perfringens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 88.5 %, Completeness (bb): 92.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.5 % (1397 of 1579)	89.6 % (731 of 816)	85.9 % (522 of 608)	92.9 % (144 of 155)
Backbone	92.9 % (780 of 840)	93.2 % (273 of 293)	92.9 % (378 of 407)	92.1 % (129 of 140)
Sidechain	85.2 % (738 of 866)	87.6 % (458 of 523)	80.8 % (265 of 328)	100.0 % (15 of 15)
Aromatic	14.3 % (14 of 98)	26.5 % (13 of 49)	2.0 % (1 of 49)
Methyl	97.4 % (148 of 152)	97.4 % (74 of 76)	97.4 % (74 of 76)

1. fiv doc

MDKTNLGELI NQGKSLLDES VEGFNVGEYH KGAKDGLTVE INKAEEVFNK EDATEEEINL AKESLEGAIA RFNSLLIEES TGDFNGNGKI DIGDLAMVSK NIGSTTNTSL DLNKDGSIDE YEISFINHRI LNLEHHHHHH

Show sample condition

Sample

Temperature 293 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	fiv_doc	[U-13C; U-15N]		1 mM

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LACS Plot; CA

Referencing offset: 0.0 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.0 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.47 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2JNK, Strand ID: A Detail

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Release date

2007-06-26

Citation

NMR assignment of backbone and side chain resonances for a dockerin-containing C-terminal fragment of the putative mu-toxin from Clostridium perfringens
Chitayat, S., Adams, J.J., Smith, S.P.
Biomol. NMR Assign. (2007), 1, 13-15, PubMed 19636814 , DOI 10.1007/s12104-007-9002-7 , Abstract

Related entities 1. fivar doc, : 1 : 1 : 7 entities Detail

Interaction partners 1. fivar doc, : 3 interactors Detail

Experiments performed 10 experiments Detail