BMRB: 7270

1H, 13C, 15N sequence-specific backbone and sidechain resonance assignments for a putative protein-protein interaction module from a family 84 glycoside hydrolase of Clostridium perfringens

Authors

Chitayat, S., Ficko-Blean, E., Adams, J.J., Gregg, K., Boraston, A.B., Smith, S.P.

Assembly

x82

Entity

1. x82 (polymer, Thiol state: not present), 165 monomers, 17423.07 Da Detail

MGSSHHHHHH SSGLVPRGSH MASKLKEAAE VTGSVSLEAL EEVQVGENLE VGVGIDELVN AEAFAYDFTL NYDENAFEYV EAISDDGVFV NAKKIEDGKV RVLVSSLTGE PLPAKEVLAK VVLRAEAKAE GSNLSVTNSS VGDGEGLVHE IAGTEKTVNI IEGTS

Formula weight

17423.07 Da

Source organism

Clostridium perfringens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.5 %, Completeness: 89.3 %, Completeness (bb): 93.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.3 % (1585 of 1775)	89.7 % (810 of 903)	87.4 % (613 of 701)	94.7 % (162 of 171)
Backbone	93.0 % (915 of 984)	93.6 % (321 of 343)	92.1 % (441 of 479)	94.4 % (153 of 162)
Sidechain	86.2 % (810 of 940)	87.3 % (489 of 560)	84.1 % (312 of 371)	100.0 % (9 of 9)
Aromatic	28.1 % (27 of 96)	39.6 % (19 of 48)	16.7 % (8 of 48)
Methyl	99.0 % (208 of 210)	99.0 % (104 of 105)	99.0 % (104 of 105)

1. x82

MGSSHHHHHH SSGLVPRGSH MASKLKEAAE VTGSVSLEAL EEVQVGENLE VGVGIDELVN AEAFAYDFTL NYDENAFEYV EAISDDGVFV NAKKIEDGKV RVLVSSLTGE PLPAKEVLAK VVLRAEAKAE GSNLSVTNSS VGDGEGLVHE IAGTEKTVNI IEGTS

Show sample condition

Sample

Temperature 293 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	x82	[U-13C; U-15N]		1 mM

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LACS Plot; CA

Referencing offset: -0.28 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.28 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.81 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2O4E, Strand ID: A Detail

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Release date

2007-06-26

Citation

NMR assignment of backbone and side chain resonances for a putative protein-protein interaction module from a family 84 glycoside hydrolase of Clostridium perfringens
Chitayat, S., Ficko-Blean, E., Adams, J.J., Gregg, K., Boraston, A.B., Smith, S.P.
Biomol. NMR Assign. (2007), 1, 7-9, PubMed 19636812 , DOI 10.1007/s12104-007-9001-8 , Abstract

Related entities 1. x82, : 1 : 3 : 12 entities Detail

Interaction partners 1. x82, : 3 interactors Detail

Experiments performed 10 experiments Detail