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BMRB: 7271

2NNZ

Backbone and sidechain 1H, 13C and 15N resonance assignments of AF2241 from Archaeoglobus fulgidus

Authors

Ai, X., Yee, A., Arrowsmith, C.H., Li, S.S.C., Choy, W.

Assembly

AF2241

Entity

1. AF2241 (polymer, Thiol state: all free), 153 monomers, 17111.04 Da Detail

MGTSHHHHHH SSGRENLYFQ GHMGERGVEM RLRIRFESAE CEVELYEEWA PETVRAIADA LPIKSTANRW GDEIYFTTQV AVEKEENSKD VVELGDVAYW IPGKAICLFF GKTPISDDKI RPASAVNVIG RIVNSMEGLK GVADGESVVV ERA

Formula weight

17111.04 Da

Source organism

Archaeoglobus fulgidus

Exptl. method

NMR

Refine. method

simulated annealing,

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.6 %, Completeness: 78.7 %, Completeness (bb): 83.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	78.7 % (1367 of 1738)	79.3 % (712 of 898)	77.0 % (525 of 682)	82.3 % (130 of 158)
Backbone	83.7 % (760 of 908)	84.7 % (266 of 314)	83.4 % (372 of 446)	82.4 % (122 of 148)
Sidechain	73.5 % (713 of 970)	75.2 % (439 of 584)	71.0 % (267 of 376)	70.0 % (7 of 10)
Aromatic	13.0 % (19 of 146)	21.9 % (16 of 73)	0.0 % (0 of 70)	100.0 % (3 of 3)
Methyl	95.8 % (159 of 166)	96.4 % (80 of 83)	95.2 % (79 of 83)

1. Structural Proteomics protein AF2241

MGTSHHHHHH SSGRENLYFQ GHMGERGVEM RLRIRFESAE CEVELYEEWA PETVRAIADA LPIKSTANRW GDEIYFTTQV AVEKEENSKD VVELGDVAYW IPGKAICLFF GKTPISDDKI RPASAVNVIG RIVNSMEGLK GVADGESVVV ERA

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.5 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	Structural Proteomics protein AF2241	[U-13C; U-15N]	protein	0.3 mM
2	NaCl		salt	450 mM
3	MOPS		buffer	10 mM
4	Zn2+		salt	10 uM
5	DTT			10 mM
6	NaN3			0.01 %
7	Inhibitor mixture			1 na
8	benzamidine			1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.2 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.2 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.59 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2NNZ, Strand ID: A Detail

Release date

2007-03-28

Citation

Backbone and side chain 1H, 13C, and 15N resonance assignments of AF2241 from Archaeoglobus fulgidus
Ai, X., Semesi, A., Yee, A., Arrowsmith, C.H., Li, S.S.C., Choy, W.
J. Biomol. NMR (2007), 38, 183-183, PubMed 17206469 , DOI 10.1007/s10858-006-9114-x , Abstract

Related entities 1. AF2241, : 1 : 3 entities Detail

Experiments performed 12 experiments Detail

1 1H15N HSQC