BMRBj - BMREntryMaster

Found 1 Document (1.864 seconds)

BMRB: 7272

2I59

Backbone and Side Chains Assignments of RGS10

Authors

Fedorov, O., Higman, V., Leidert, M., Elkins, J., Soundararajan, M., Doyle, D., Schmieder, P., Sundstrom, M., Arrowsmith, C., Weigelt, J., Edwards, A., Diehl, A., Oschkinat, H., Ball, L.

Assembly

RGS10

Entity

1. RGS10 (polymer, Thiol state: all free), 138 monomers, 16486.60 Da Detail

SMQSLKSTAK WAASLENLLE DPEGVKRFRE FLKKEFSEEN VLFWLACEDF KKMQDKTQMQ EKAKEIYMTF LSSKASSQVN VEGQSRLNEK ILEEPHPLMF QKLQDQIFNL MKYDSYSRFL KSDLFLKHKR TEEEEEDL

Formula weight

16486.6 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.4 %, Completeness: 87.4 %, Completeness (bb): 94.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.4 % (1522 of 1742)	85.2 % (787 of 924)	88.5 % (590 of 667)	96.0 % (145 of 151)
Backbone	94.4 % (776 of 822)	93.8 % (258 of 275)	94.2 % (388 of 412)	96.3 % (130 of 135)
Sidechain	82.8 % (874 of 1056)	81.5 % (529 of 649)	84.4 % (330 of 391)	93.8 % (15 of 16)
Aromatic	74.4 % (116 of 156)	75.6 % (59 of 78)	72.4 % (55 of 76)	100.0 % (2 of 2)
Methyl	89.7 % (104 of 116)	89.7 % (52 of 58)	89.7 % (52 of 58)

1. RGS10

SMQSLKSTAK WAASLENLLE DPEGVKRFRE FLKKEFSEEN VLFWLACEDF KKMQDKTQMQ EKAKEIYMTF LSSKASSQVN VEGQSRLNEK ILEEPHPLMF QKLQDQIFNL MKYDSYSRFL KSDLFLKHKR TEEEEEDL

Show sample condition

Sample

Temperature 297 K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	RGS10	[U-13C; U-15N]	1.0 mM
2	Na phosphate buffer		20 mM
3	NaCl		50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.25 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2I59, Strand ID: A Detail

Release date

2007-01-08

Citation

Resonance assignment of the RGS domain of human RGS10
Fedorov, O.Y., Higman, V.A., Schmieder, P., Leidert, M., Diehl, A., Elkins, J., Soundararajan, M., Oschkinat, H., Ball, L.J.
J. Biomol. NMR (2007), 38, 191-191, PubMed 17180548 , DOI 10.1007/s10858-006-9111-0 , Abstract

Related entities 1. RGS10, : 1 : 4 : 235 entities Detail

Interaction partners 1. RGS10, : 4 interactors Detail

More details for 4 interactors identified by 2 citations

Experiments performed 15 experiments Detail

1 1H15N HSQC