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BMRB: 7287

2I7K

Backbone and side chain 1H, 13C and 15N Chemical Shift Assignments for human BRD7 bromodomain

Authors

Sun, H., Liu, J., Zhang, J., Huang, H., Xu, C., Dai, H., Wu, J., Shi, Y.

Assembly

BRD7 Bromodomain

Entity

1. BRD7 Bromodomain (polymer, Thiol state: all free), 117 monomers, 13894.88 Da Detail

MEEVEQTPLQ EALNQLMRQL QRKDPSAFFS FPVTDFIAPG YSMIIKHPMD FSTMKEKIKN NDYQSIEELK DNFKLMCTNA MIYNKPETIY YKAAKKLLHS GMKILSQERL EHHHHHH

Formula weight

13894.88 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.9 %, Completeness: 85.1 %, Completeness (bb): 93.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.1 % (1243 of 1460)	87.9 % (678 of 771)	79.5 % (449 of 565)	93.5 % (116 of 124)
Backbone	93.3 % (644 of 690)	93.5 % (215 of 230)	93.1 % (325 of 349)	93.7 % (104 of 111)
Sidechain	79.9 % (707 of 885)	85.6 % (463 of 541)	70.1 % (232 of 331)	92.3 % (12 of 13)
Aromatic	34.1 % (45 of 132)	60.6 % (40 of 66)	7.6 % (5 of 66)
Methyl	94.1 % (96 of 102)	96.1 % (49 of 51)	92.2 % (47 of 51)

1. bromodomain

MEEVEQTPLQ EALNQLMRQL QRKDPSAFFS FPVTDFIAPG YSMIIKHPMD FSTMKEKIKN NDYQSIEELK DNFKLMCTNA MIYNKPETIY YKAAKKLLHS GMKILSQERL EHHHHHH

Show sample condition

Sample

Temperature 293 (±0.2) K, pH 6.1

#	Name	Isotope labeling	Concentration
1	bromodomain	[U-13C; U-15N]	0.6 mM
2	phosphate buffer		50 mM
3	NaCl		50 mM
4	DTT		1 mM
5	EDTA		5 mM
6	H2O		90 %
7	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	H2O	protons	0.0 ppm	null	null	0.2514495
¹H	H2O	protons	4.81 ppm	internal	indirect	1.0
¹⁵N	H2O	protons	0.0 ppm	null	null	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	H2O	protons	0.0 ppm	null	null	0.2514495
¹H	H2O	protons	4.81 ppm	internal	indirect	1.0
¹⁵N	H2O	protons	0.0 ppm	null	null	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	H2O	protons	0.0 ppm	null	null	0.2514495
¹H	H2O	protons	4.81 ppm	internal	indirect	1.0
¹⁵N	H2O	protons	0.0 ppm	null	null	0.1013291

Referencing offset: 0.06 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	H2O	protons	0.0 ppm	null	null	0.2514495
¹H	H2O	protons	4.81 ppm	internal	indirect	1.0
¹⁵N	H2O	protons	0.0 ppm	null	null	0.1013291

Referencing offset: 0.19 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2I7K, Strand ID: A Detail

Release date

2007-01-09

Citation

Solution structure of BRD7 bromodomain and its interaction with acetylated peptides from histone H3 and H4
Sun, H., Liu, J., Zhang, J., Shen, W., Huang, H., Xu, C., Dai, H., Wu, J., Shi, Y.
Biochem. Biophys. Res. Commun. (2007), 358, 435-441, PubMed 17498659 , DOI 10.1016/j.bbrc.2007.04.139 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q9NPI1 released on 2006-03-21
Title BRD7_HUMAN Entity Bromodomain-containing protein 7

Related entities 1. BRD7 Bromodomain, : 1 : 171 entities Detail

Interaction partners 1. BRD7 Bromodomain, : 5 interactors Detail

More details for 5 interactors identified by 3 citations

Experiments performed 13 experiments Detail

1 1H15N HSQC