BMRBj - BMREntryMaster

Found 1 Document (1.861 seconds)

BMRB: 7288

2J5D

1H, 13C, 15N Chemical Shift Assignments and 15N Relaxation Data for Transmembrane Domain of BNIP3

Authors

Pustovalova, J.E., Bocharov, E.V., Maslennikov, I.V., Ermolyuk, Y.S., Goncharuk, M.V., Arseniev, A.S.

Assembly

BNIP3 TM dimer

Entity

1. BNIP3 TM dimer (polymer, Thiol state: not present), 45 monomers, 4919.874 × 2 Da Detail

RNTSVMKKGG IFSAEFLKVF LPSLLLSHLL AIGLGIYIGR RLTTS

Total weight

9839.748 Da

Max. entity weight

4919.874 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

TAD

Data set

assigned_chemical_shifts, heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation, H_exch_rates

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 76.9 %, Completeness (bb): 78.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	76.9 % (415 of 540)	91.4 % (254 of 278)	54.4 % (118 of 217)	95.6 % (43 of 45)
Backbone	78.7 % (211 of 268)	93.6 % (88 of 94)	62.3 % (81 of 130)	95.5 % (42 of 44)
Sidechain	76.6 % (239 of 312)	90.2 % (166 of 184)	56.7 % (72 of 127)	100.0 % (1 of 1)
Aromatic	61.9 % (26 of 42)	100.0 % (21 of 21)	23.8 % (5 of 21)
Methyl	68.6 % (48 of 70)	88.6 % (31 of 35)	48.6 % (17 of 35)

1. BNIP3 TM

RNTSVMKKGG IFSAEFLKVF LPSLLLSHLL AIGLGIYIGR RLTTS

Show sample condition

Sample #1

Temperature 313 (±2) K, pH 5.10 (±0.05), Details peptide in lipid bicelles (DHPC/DMPC)

#	Name	Isotope labeling	Type	Concentration
1	BNIP3 TM	[U-95% 13C; U-95% 15N]	protein	1.38 (±0.02) mM
2	DHPC		lipid	44.13 (±0.02) mM
3	DMPC		lipid	11.03 (±0.02) mM
4	Acetic buffer		buffer	20 mM
5	NaN3		antimicrobial agent	1.5 mM
6	EDTA		chelating agent	1 mM

Sample #2

Temperature 313 (±2) K, pH 5.10 (±0.05), Details peptide in lipid bicelles (DHPC/DMPC)

#	Name	Isotope labeling	Type	Concentration
7	BNIP3 TM	[U-95% 15N]	protein	1.38 (±0.02) mM
8	DHPC		lipid	44.13 (±0.02) mM
9	DMPC		lipid	11.03 (±0.02) mM
10	Acetic buffer		buffer	20 mM
11	NaN3		antimicrobial agent	1.5 mM
12	EDTA		chelating agent	1 mM

Protein Blocks Logo

Calculated from 16 models in PDB: 2J5D, Strand ID: A, B Detail

Heteronucl. T₁

38 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 600 MHz, Temperature 313 (±2) K, pH 5.10 (±0.05) Detail

Heteronucl. T₂

38 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 600 MHz, Temperature 313 (±2) K, pH 5.10 (±0.05) Detail

Heteronucl. NOE

38 NOE values in 1 lists
Value type na, Field strength (¹H) 600 MHz, Temperature 313 (±2) K, pH 5.10 (±0.05) Detail

Heteronucl. T₁/T₂

38 T₁/T₂ values in 1 lists
Field strength (¹H) 600 MHz, Temperature 313 (±2) K, pH 5.10 (±0.05) Detail

¹H exchange rate

44 k_ex values in 1 lists
Temperature 313 (±2) K, pH 5.10 (±0.05) Detail

Release date

2007-05-09

Citation

Unique dimeric structure of BNip3 transmembrane domain suggests membrane permeabilization as a cell death trigger
Bocharov, E.V., Pustovalova, Y.E., Pavlov, K.V., Volynsky, P.E., Goncharuk, M.V., Ermolyuk, Y.S., Karpunin, D.V., Schulga, A.A., Kirpichnikov, M.P., Efremov, R.G., Maslennikov, I.V., Arseniev, A.S.
J. Biol. Chem. (2007), 282, 16256-16266, PubMed 17412696 , DOI 10.1074/jbc.M701745200 , Abstract

Related entities 1. BNIP3 TM dimer, : 1 : 3 : 3 : 10 entities Detail

Interaction partners 1. BNIP3 TM dimer, : 12 interactors Detail

More details for 12 interactors identified by 5 citations

Experiments performed 5 experiments Detail

1 1H15N HSQC

Instrument

Model Varian UNITY (600 MHz)

Sample #1

Temperature 313 (±2) K, pH 5.10 (±0.05), Details peptide in lipid bicelles (DHPC/DMPC)

#	Name	Isotope labeling	Type	Concentration
1	BNIP3 TM	[U-95% 13C; U-95% 15N]	protein	1.38 (±0.02) mM
2	DHPC		lipid	44.13 (±0.02) mM
3	DMPC		lipid	11.03 (±0.02) mM
4	Acetic buffer		buffer	20 mM
5	NaN3		antimicrobial agent	1.5 mM
6	EDTA		chelating agent	1 mM

Sample #2

Temperature 313 (±2) K, pH 5.10 (±0.05), Details peptide in lipid bicelles (DHPC/DMPC)

#	Name	Isotope labeling	Type	Concentration
7	BNIP3 TM	[U-95% 15N]	protein	1.38 (±0.02) mM
8	DHPC		lipid	44.13 (±0.02) mM
9	DMPC		lipid	11.03 (±0.02) mM
10	Acetic buffer		buffer	20 mM
11	NaN3		antimicrobial agent	1.5 mM
12	EDTA		chelating agent	1 mM

Sample #3

Temperature 313 (±2) K, pH 5.10 (±0.05), Details peptide in lipid bicelles (DHPC/DMPC)

#	Name	Isotope labeling	Type	Concentration
13	BNIP3 TM	[U-95% 13C; U-95% 15N]	protein	0.69 (±0.02) mM
14	DHPC		lipid	44.13 (±0.02) mM
15	DMPC		lipid	11.03 (±0.02) mM
16	Acetic buffer		buffer	20 mM
17	NaN3		antimicrobial agent	1.5 mM
18	EDTA		chelating agent	1 mM

2 3D 15N-NOESY

Instrument

Model Varian UNITY (600 MHz)

Sample #1

Temperature 313 (±2) K, pH 5.10 (±0.05), Details peptide in lipid bicelles (DHPC/DMPC)

#	Name	Isotope labeling	Type	Concentration
1	BNIP3 TM	[U-95% 13C; U-95% 15N]	protein	1.38 (±0.02) mM
2	DHPC		lipid	44.13 (±0.02) mM
3	DMPC		lipid	11.03 (±0.02) mM
4	Acetic buffer		buffer	20 mM
5	NaN3		antimicrobial agent	1.5 mM
6	EDTA		chelating agent	1 mM

Sample #2

Temperature 313 (±2) K, pH 5.10 (±0.05), Details peptide in lipid bicelles (DHPC/DMPC)

#	Name	Isotope labeling	Type	Concentration
7	BNIP3 TM	[U-95% 15N]	protein	1.38 (±0.02) mM
8	DHPC		lipid	44.13 (±0.02) mM
9	DMPC		lipid	11.03 (±0.02) mM
10	Acetic buffer		buffer	20 mM
11	NaN3		antimicrobial agent	1.5 mM
12	EDTA		chelating agent	1 mM

Sample #3

Temperature 313 (±2) K, pH 5.10 (±0.05), Details peptide in lipid bicelles (DHPC/DMPC)

#	Name	Isotope labeling	Type	Concentration
13	BNIP3 TM	[U-95% 13C; U-95% 15N]	protein	0.69 (±0.02) mM
14	DHPC		lipid	44.13 (±0.02) mM
15	DMPC		lipid	11.03 (±0.02) mM
16	Acetic buffer		buffer	20 mM
17	NaN3		antimicrobial agent	1.5 mM
18	EDTA		chelating agent	1 mM

3 1H13C HSQC

Instrument

Model Varian UNITY (600 MHz)

Sample #1

Temperature 313 (±2) K, pH 5.10 (±0.05), Details peptide in lipid bicelles (DHPC/DMPC)

#	Name	Isotope labeling	Type	Concentration
1	BNIP3 TM	[U-95% 13C; U-95% 15N]	protein	1.38 (±0.02) mM
2	DHPC		lipid	44.13 (±0.02) mM
3	DMPC		lipid	11.03 (±0.02) mM
4	Acetic buffer		buffer	20 mM
5	NaN3		antimicrobial agent	1.5 mM
6	EDTA		chelating agent	1 mM

Sample #2

Temperature 313 (±2) K, pH 5.10 (±0.05), Details peptide in lipid bicelles (DHPC/DMPC)

#	Name	Isotope labeling	Type	Concentration
7	BNIP3 TM	[U-95% 15N]	protein	1.38 (±0.02) mM
8	DHPC		lipid	44.13 (±0.02) mM
9	DMPC		lipid	11.03 (±0.02) mM
10	Acetic buffer		buffer	20 mM
11	NaN3		antimicrobial agent	1.5 mM
12	EDTA		chelating agent	1 mM

Sample #3

Temperature 313 (±2) K, pH 5.10 (±0.05), Details peptide in lipid bicelles (DHPC/DMPC)

#	Name	Isotope labeling	Type	Concentration
13	BNIP3 TM	[U-95% 13C; U-95% 15N]	protein	0.69 (±0.02) mM
14	DHPC		lipid	44.13 (±0.02) mM
15	DMPC		lipid	11.03 (±0.02) mM
16	Acetic buffer		buffer	20 mM
17	NaN3		antimicrobial agent	1.5 mM
18	EDTA		chelating agent	1 mM

4 3D 13C-NOESY

Instrument

Model Varian UNITY (600 MHz)

Sample #1

Temperature 313 (±2) K, pH 5.10 (±0.05), Details peptide in lipid bicelles (DHPC/DMPC)

#	Name	Isotope labeling	Type	Concentration
1	BNIP3 TM	[U-95% 13C; U-95% 15N]	protein	1.38 (±0.02) mM
2	DHPC		lipid	44.13 (±0.02) mM
3	DMPC		lipid	11.03 (±0.02) mM
4	Acetic buffer		buffer	20 mM
5	NaN3		antimicrobial agent	1.5 mM
6	EDTA		chelating agent	1 mM

Sample #2

Temperature 313 (±2) K, pH 5.10 (±0.05), Details peptide in lipid bicelles (DHPC/DMPC)

#	Name	Isotope labeling	Type	Concentration
7	BNIP3 TM	[U-95% 15N]	protein	1.38 (±0.02) mM
8	DHPC		lipid	44.13 (±0.02) mM
9	DMPC		lipid	11.03 (±0.02) mM
10	Acetic buffer		buffer	20 mM
11	NaN3		antimicrobial agent	1.5 mM
12	EDTA		chelating agent	1 mM

Sample #3

Temperature 313 (±2) K, pH 5.10 (±0.05), Details peptide in lipid bicelles (DHPC/DMPC)

#	Name	Isotope labeling	Type	Concentration
13	BNIP3 TM	[U-95% 13C; U-95% 15N]	protein	0.69 (±0.02) mM
14	DHPC		lipid	44.13 (±0.02) mM
15	DMPC		lipid	11.03 (±0.02) mM
16	Acetic buffer		buffer	20 mM
17	NaN3		antimicrobial agent	1.5 mM
18	EDTA		chelating agent	1 mM

5 HCCH-TOCSY

Instrument

Model Varian UNITY (600 MHz)

Sample #1

Temperature 313 (±2) K, pH 5.10 (±0.05), Details peptide in lipid bicelles (DHPC/DMPC)

#	Name	Isotope labeling	Type	Concentration
1	BNIP3 TM	[U-95% 13C; U-95% 15N]	protein	1.38 (±0.02) mM
2	DHPC		lipid	44.13 (±0.02) mM
3	DMPC		lipid	11.03 (±0.02) mM
4	Acetic buffer		buffer	20 mM
5	NaN3		antimicrobial agent	1.5 mM
6	EDTA		chelating agent	1 mM

Sample #2

Temperature 313 (±2) K, pH 5.10 (±0.05), Details peptide in lipid bicelles (DHPC/DMPC)

#	Name	Isotope labeling	Type	Concentration
7	BNIP3 TM	[U-95% 15N]	protein	1.38 (±0.02) mM
8	DHPC		lipid	44.13 (±0.02) mM
9	DMPC		lipid	11.03 (±0.02) mM
10	Acetic buffer		buffer	20 mM
11	NaN3		antimicrobial agent	1.5 mM
12	EDTA		chelating agent	1 mM

Sample #3

Temperature 313 (±2) K, pH 5.10 (±0.05), Details peptide in lipid bicelles (DHPC/DMPC)

#	Name	Isotope labeling	Type	Concentration
13	BNIP3 TM	[U-95% 13C; U-95% 15N]	protein	0.69 (±0.02) mM
14	DHPC		lipid	44.13 (±0.02) mM
15	DMPC		lipid	11.03 (±0.02) mM
16	Acetic buffer		buffer	20 mM
17	NaN3		antimicrobial agent	1.5 mM
18	EDTA		chelating agent	1 mM

nullKeywords apoptotic, BNIP3, apoptosis, dynamics, structure

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Found 1 Document (1.861 seconds)

BMRB: 7288

Sample #1

Sample #2

Related entities 1. BNIP3 TM dimer, : 1 : 3 : 3 : 10 entities Detail

Interaction partners 1. BNIP3 TM dimer, : 12 interactors Detail

Experiments performed 5 experiments Detail

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