BMRB: 7293

Backbone 1H, 13C, and 15N chemical shift assignments for recoverin bound to rhodopsin kinase

Authors

Ames, J.B.

Assembly

recoverin bound to rhodopsin kinase fragment (RK25)

Entity

1. recoverin (polymer, Thiol state: all free), 202 monomers, 23333.10 Da Detail

MGNSKSGALS KEILEELQLN TKFTEEELSS WYQSFLKECP SGRITRQEFQ TIYSKFFPEA DPKAYAQHVF RSFDANSDGT LDFKEYVIAL HMTSAGKTNQ KLEWAFSLYD VDGNGTISKN EVLEIVTAIF KMISPEDTKH LPEDENTPEK RAEKIWGFFG KKDDDKLTEK EFIEGTLANK EILRLIQFEP QKVKEKLKEK KL

2. rk25 (polymer, Thiol state: all free), 25 monomers, 2563.793 Da Detail

MDFGSLETVV ANSAFIAARG SFDAS

3. CALCIUM ION (non-polymer), 40.078 × 2 Da

Total weight

25977.049 Da

Max. entity weight

23333.1 Da

Source organism

Bos taurus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.0 %, Completeness: 39.2 %, Completeness (bb): 72.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	39.2 % (1071 of 2732)	30.7 % (439 of 1428)	41.4 % (441 of 1064)	79.6 % (191 of 240)
Backbone	72.8 % (981 of 1348)	83.0 % (381 of 459)	61.4 % (411 of 669)	85.9 % (189 of 220)
Sidechain	7.0 % (112 of 1599)	5.8 % (56 of 969)	8.9 % (54 of 610)	10.0 % (2 of 20)
Aromatic	2.7 % (7 of 258)	3.9 % (5 of 129)	0.8 % (1 of 126)	33.3 % (1 of 3)
Methyl	14.1 % (31 of 220)	11.8 % (13 of 110)	16.4 % (18 of 110)

1. recoverin

MGNSKSGALS KEILEELQLN TKFTEEELSS WYQSFLKECP SGRITRQEFQ TIYSKFFPEA DPKAYAQHVF RSFDANSDGT LDFKEYVIAL HMTSAGKTNQ KLEWAFSLYD VDGNGTISKN EVLEIVTAIF KMISPEDTKH LPEDENTPEK RAEKIWGFFG KKDDDKLTEK EFIEGTLANK EILRLIQFEP QKVKEKLKEK KL

2. rk25

MDFGSLETVV ANSAFIAARG SFDAS

Show sample condition

Sample

Temperature 310 (±0.1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	recoverin	[U-95% 13C; U-90% 15N]	protein	0.5 mM
2	rk25	[U-95%; U-90% 15N]	protein	0.5 mM

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Protein Blocks Logo

Calculated from 10 models in PDB: 2I94, Strand ID: A, B Detail

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Release date

2006-12-19

Citation

Structural basis for calcium-induced inhibition of rhodopsin kinase by recoverin
Ames, J.B., Levay, K.B., Wingard, J.N., Lusin, J.D., Slepak, V.Z.
J. Biol. Chem. (2006), 281, 37237-37245, PubMed 17020884 , DOI 10.1074/jbc.M606913200 , Abstract

Related entities 1. recoverin, : 93 entities Detail

Related entities 2. rk25, : 1 : 9 : 9 : 7 entities Detail

Interaction partners 2. rk25, : 3 interactors Detail

Experiments performed 6 experiments Detail