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BMRB: 7297

2IDA

Assignment of conserved hypothetical protein RPA1320 from Rhodopseudomonas Palustris; Northeast Structural Genomics Consortium Target RPT3 / Ontario Center for Structural Proteomics Target RP1313

Authors

Lemak, A., Yee, A., Lukin, J., Karra, M., Gutmanas, A., Guido, V., Arrowsmith, C.H.

Assembly

conserved hypotetical protein RPA1320

Entity

1. conserved hypotetical protein rpa1320 (polymer, Thiol state: free and other bound), 102 monomers, 11411.97 Da Detail

MTMGCRHVAG IRTVTPSALG CEECLKIGSP WVHLRICRTC GHVGCCDDSP HKHATRHFHA TGHPIIEGYD PPEGWGWCYV DEVMFDLSDR MTPHNGPIPR YV

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

11542.788 Da

Max. entity weight

11411.97 Da

Entity Connection

na 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS37:SG	2:ZN1:ZN
2	na	sing	1:CYS40:SG	2:ZN1:ZN
3	na	sing	1:HIS57:NE2	2:ZN1:ZN
4	na	sing	1:HIS63:ND1	2:ZN1:ZN
5	na	sing	1:CYS5:SG	2:ZN1:ZN
6	na	sing	1:CYS78:SG	2:ZN1:ZN
7	na	sing	1:HIS7:ND1	2:ZN1:ZN
8	na	sing	1:ASP81:OD1	2:ZN1:ZN

Source organism

Rhodopseudomonas palustris CGA009

Exptl. method

NMR

Refine. method

molecular dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.1 %, Completeness (bb): 96.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.1 % (1068 of 1135)	94.9 % (556 of 586)	92.5 % (418 of 452)	96.9 % (94 of 97)
Backbone	96.6 % (574 of 594)	98.5 % (203 of 206)	95.3 % (281 of 295)	96.8 % (90 of 93)
Sidechain	92.6 % (585 of 632)	92.9 % (353 of 380)	91.9 % (228 of 248)	100.0 % (4 of 4)
Aromatic	72.4 % (84 of 116)	72.4 % (42 of 58)	70.9 % (39 of 55)	100.0 % (3 of 3)
Methyl	100.0 % (90 of 90)	100.0 % (45 of 45)	100.0 % (45 of 45)

1. conserved hypotetical protein rpa1320

MTMGCRHVAG IRTVTPSALG CEECLKIGSP WVHLRICRTC GHVGCCDDSP HKHATRHFHA TGHPIIEGYD PPEGWGWCYV DEVMFDLSDR MTPHNGPIPR YV

Show sample condition

Sample

Temperature 298 (±1) K, pH 7.3 (±0.2)

#	Name	Isotope labeling	Concentration
1	conserved hypotetical protein rpa1320	[U-13C; U-15N]	0.6 mM
2	NaCl		250 mM
3	TRIS		10 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.51 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.51 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2IDA, Strand ID: A Detail

Release date

2006-10-22

Citation

Solution structure of RPA1320
Lemak, A., Yee, A., Lukin, J., Karra, M., Gutmanas, A., Guido, V., Arrowsmith, C.H.

Related entities 1. conserved hypotetical protein rpa1320, : 1 : 6 entities Detail

Experiments performed 9 experiments Detail

1 15N HSQC