BMRB: 7298

1H, 13C, 15N-NMR resonance assignments for Znf-UBP domain of Ubp-M

Authors

Pai, M., Zhou, P.

Assembly

Znf-UBP domain of Ubp-M

Entity

1. Ubp-M Znf-UBP domain (polymer, Thiol state: all free), 126 monomers, 14459.23 Da Detail

GSHMPVCRHI RKGLEQGNLK KALVNVEWNI CQDCKTDNKV KDKAEEETEE KPSVWLCLKC GHQGCGRNSQ EQHALKHYLT PRSEPHCLVL SLDNWSVWCY VCDNEVQYCS SNQLGQVVDY VRKQAS

2. ZN (non-polymer), 65.409 × 3 Da

Total weight

14655.457 Da

Max. entity weight

14459.23 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 90.0 %, Completeness (bb): 83.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.0 % (1326 of 1474)	96.6 % (748 of 774)	79.0 % (440 of 557)	96.5 % (138 of 143)
Backbone	83.8 % (627 of 748)	96.1 % (245 of 255)	71.4 % (265 of 371)	95.9 % (117 of 122)
Sidechain	96.4 % (815 of 845)	96.9 % (503 of 519)	95.4 % (291 of 305)	100.0 % (21 of 21)
Aromatic	89.4 % (93 of 104)	98.1 % (51 of 52)	79.2 % (38 of 48)	100.0 % (4 of 4)
Methyl	100.0 % (114 of 114)	100.0 % (57 of 57)	100.0 % (57 of 57)

1. Ubp-M Znf-UBP domain

GSHMPVCRHI RKGLEQGNLK KALVNVEWNI CQDCKTDNKV KDKAEEETEE KPSVWLCLKC GHQGCGRNSQ EQHALKHYLT PRSEPHCLVL SLDNWSVWCY VCDNEVQYCS SNQLGQVVDY VRKQAS

Show sample condition

Sample

Temperature 300 (±0.2) K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	Ubp-M Znf-UBP domain	[U-13C; U-15N]		1.0 mM
2	phosphate			25 mM
3	KCl			100 mM
4	D2O			100 %

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LACS Plot; CA

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2I50, Strand ID: A Detail

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Release date

2008-05-11

Citation

Solution structure of the Ubp-M BUZ domain, a highly specific protein module that recognizes the C-terminal tail of free ubiquitin
Pai, M., Tzeng, S., Kovacs, J.J., Keaton, M.A., Li, S.S.-C., Yao, T., Zhou, P.
J. Mol. Biol. (2007), 370, 290-302, PubMed 17512543 , DOI 10.1016/j.jmb.2007.04.015 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Ubp-M Znf-UBP domain, : 1 : 2 : 24 entities Detail

Interaction partners 1. Ubp-M Znf-UBP domain, : 4 interactors Detail

Experiments performed 8 experiments Detail