BMRB: 7299

Mouse Cripto CFC domain solution structure

Authors

Calvanese, L., Saporito, A., Marasco, D., D'Auria, G., Minchiotti, G., Pedone, C., Paolillo, L., Falcigno, L., Ruvo, M.

Assembly

cfc

Entity

1. cfc (polymer, Thiol state: all disulfide bound), 41 monomers, 4474.286 Da Detail

XKEHCGSILH GTWLPKKCSL CRCWHGQLHC LIQTFLPGCD X

Formula weight

4474.286 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS5:SG	1:CYS23:SG
2	disulfide	sing	1:CYS18:SG	1:CYS39:SG
3	disulfide	sing	1:CYS21:SG	1:CYS30:SG

Source organism

Mus musculus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.1 %, Completeness: 86.3 %, Completeness (bb): 90.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	86.3 % (207 of 240)	86.3 % (207 of 240)
Backbone	90.0 % (72 of 80)	90.0 % (72 of 80)
Sidechain	84.4 % (135 of 160)	84.4 % (135 of 160)
Aromatic	88.0 % (22 of 25)	88.0 % (22 of 25)
Methyl	77.8 % (14 of 18)	77.8 % (14 of 18)

1. CFC

XKEHCGSILH GTWLPKKCSL CRCWHGQLHC LIQTFLPGCD X

Show sample condition

Sample

Temperature 298 (±0.2) K, pH 6, Details H2O/D2O 90/10, TSP, pH 6

#	Name	Isotope labeling	Type	Concentration
1	CFC		protein	1.5 mM
2	H2O		solvent	90 %
3	D2O		solvent	10 %

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Protein Blocks Logo

Calculated from 10 models in PDB: 2J5H, Strand ID: A Detail

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Release date

2007-03-05

Citation

Solution structure of mouse Cripto CFC domain and its inactive variant Trp107Ala
Calvanese, L., Saporito, A., Marasco, D., D'Auria, G., Minchiotti, G., Pedone, C., Paolillo, L., Falcigno, L., Ruvo, M.
J. Med. Chem. (2006), 49, 7054-7062, PubMed 17125258 , DOI 10.1021/jm060772r , Abstract

Related entities 1. cfc, : 1 : 2 : 15 entities Detail

Interaction partners 1. cfc, : 5 interactors Detail

Experiments performed 3 experiments Detail