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BMRB: 7302

2I4K

The PX domain of Sorting Nexin 1 (SNX1)

Authors

Watson, M.J., Waltho, J.P., Hounslow, A.M.

Assembly

SNX1-PX

Entity

1. SNX1-PX (polymer, Thiol state: not present), 128 monomers, 14679.60 Da Detail

QFDLTVGITD PEKIGDGMNA YVAYKVTTQT SLPLFRSKQF AVKRRFSDFL GLYEKLSEKH SQNGFIVPPP PEKSLIGMTK VKVGKEDSSS AEFLEKRRAA LERYLQRIVN HPTMLQDPDV REFLEKEE

Formula weight

14679.6 Da

Source organism

Exptl. method

Refine. method

molecular dynamics, simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.7 %, Completeness: 85.5 %, Completeness (bb): 96.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.5 % (1327 of 1552)	79.5 % (650 of 818)	91.4 % (553 of 605)	96.1 % (124 of 129)
Backbone	96.0 % (722 of 752)	92.9 % (237 of 255)	97.1 % (366 of 377)	99.2 % (119 of 120)
Sidechain	78.4 % (722 of 921)	73.4 % (413 of 563)	87.1 % (304 of 349)	55.6 % (5 of 9)
Aromatic	61.7 % (74 of 120)	65.0 % (39 of 60)	58.3 % (35 of 60)
Methyl	98.5 % (128 of 130)	98.5 % (64 of 65)	98.5 % (64 of 65)

1. SNX1-PX

QFDLTVGITD PEKIGDGMNA YVAYKVTTQT SLPLFRSKQF AVKRRFSDFL GLYEKLSEKH SQNGFIVPPP PEKSLIGMTK VKVGKEDSSS AEFLEKRRAA LERYLQRIVN HPTMLQDPDV REFLEKEE

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SNX1-PX	[U-13C; U-15N]	protein	1 mM
2	Phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	NaN3		antimicrobial agent	1 mM
5	D20		solvent	10 %

LACS Plot; CA

Referencing offset: -0.02 ppm, Outliers: 7 Detail

LACS Plot; CB

Referencing offset: -0.02 ppm, Outliers: 7 Detail

LACS Plot; HA

Referencing offset: 0.12 ppm, Outliers: 3 Detail

LACS Plot; CO

Referencing offset: -0.02 ppm, Outliers: 5 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2I4K, Strand ID: A Detail

Release date

2006-11-13

Citation

Determinants of the endosomal localization of sorting nexin 1
Zhong, Q., Watson, M.J., Lazar, C.S., Hounslow, A.M., Waltho, J.P., Gill, G.N.
Mol. Biol. Cell (2005), 16, 2049-2057, PubMed 15673616 , DOI 10.1091/mbc.E04-06-0504 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q13596 released on 2000-12-01
Title SNX1_HUMAN Entity Sorting nexin-1

Related entities 1. SNX1-PX, : 1 : 3 : 5 : 230 entities Detail

Interaction partners 1. SNX1-PX, : 25 interactors Detail

More details for 25 interactors identified by 12 citations

Experiments performed 10 experiments Detail

1 15N-HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SNX1-PX	[U-13C; U-15N]	protein	1 mM
2	Phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	NaN3		antimicrobial agent	1 mM
5	D20		solvent	10 %

2 13C-HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SNX1-PX	[U-13C; U-15N]	protein	1 mM
2	Phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	NaN3		antimicrobial agent	1 mM
5	D20		solvent	10 %

3 HNCA

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SNX1-PX	[U-13C; U-15N]	protein	1 mM
2	Phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	NaN3		antimicrobial agent	1 mM
5	D20		solvent	10 %

4 HNCO

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SNX1-PX	[U-13C; U-15N]	protein	1 mM
2	Phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	NaN3		antimicrobial agent	1 mM
5	D20		solvent	10 %

5 HNCACB

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SNX1-PX	[U-13C; U-15N]	protein	1 mM
2	Phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	NaN3		antimicrobial agent	1 mM
5	D20		solvent	10 %

6 CBCA(CO)NH

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SNX1-PX	[U-13C; U-15N]	protein	1 mM
2	Phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	NaN3		antimicrobial agent	1 mM
5	D20		solvent	10 %

7 HN(CA)CO

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SNX1-PX	[U-13C; U-15N]	protein	1 mM
2	Phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	NaN3		antimicrobial agent	1 mM
5	D20		solvent	10 %

8 CCH-TOCSY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SNX1-PX	[U-13C; U-15N]	protein	1 mM
2	Phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	NaN3		antimicrobial agent	1 mM
5	D20		solvent	10 %

9 HCCH-TOCSY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SNX1-PX	[U-13C; U-15N]	protein	1 mM
2	Phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	NaN3		antimicrobial agent	1 mM
5	D20		solvent	10 %

10 15N,13C-NOESY-HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Temperature 298 (±0.1) K, pH 6.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	SNX1-PX	[U-13C; U-15N]	protein	1 mM
2	Phosphate		buffer	20 mM
3	NaCl		salt	100 mM
4	NaN3		antimicrobial agent	1 mM
5	D20		solvent	10 %

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