BMRB: 7305

R21A Spc-SH3 free

Authors

van Nuland, N.A.J., Casares, S., AB, E., Eshuis, H., Lopez-Mayorga, O., Conejero-Lara, F.

Assembly

R21A Spc-SH3 monomer

Entity

1. R21A Spc-SH3 monomer (polymer, Thiol state: not present), 62 monomers, 7134.053 Da Detail

MDETGKELVL ALYDYQEKSP AEVTMKKGDI LTLLNSTNKD WWKVEVNDRQ GFVPAAYVKK LD

Formula weight

7134.053 Da

Source organism

Gallus gallus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.5 %, Completeness (bb): 99.2 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H
All	99.5 % (387 of 389)	99.5 % (387 of 389)
Backbone	99.2 % (124 of 125)	99.2 % (124 of 125)
Sidechain	99.6 % (263 of 264)	99.6 % (263 of 264)
Aromatic	100.0 % (29 of 29)	100.0 % (29 of 29)
Methyl	100.0 % (36 of 36)	100.0 % (36 of 36)

1. R21A Spc-SH3 free

MDETGKELVL ALYDYQEKSP AEVTMKKGDI LTLLNSTNKD WWKVEVNDRQ GFVPAAYVKK LD

Show sample condition

Sample

Temperature 300 (±0.1) K, pH 3.5 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	R21A Spc-SH3 free			2 mM
2	H2O			90 %
3	D2O			10 %
4	d5-glycine			20 mM

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 2JM8, Strand ID: A Detail

---

Release date

2007-05-03

Citation

The high-resolution NMR structure of the R21A Spc-SH3:P41 complex: understanding the determinants of binding affinity by comparison with Abl-SH3
Casares, S., AB, E., Eshuis, H., Lopez-Mayorga, O., van Nuland, N.A.J., Conejero-Lara, F.
BMC Struct. Biol. (2007), 7, 22-22, PubMed 17407569 , DOI 10.1186/1472-6807-7-22 , Abstract

Related entities 1. R21A Spc-SH3 monomer, : 1 : 3 : 43 : 264 entities Detail

Interaction partners 1. R21A Spc-SH3 monomer, : 121 interactors Detail

Experiments performed 2 experiments Detail