BMRBj - BMREntryMaster

Found 1 Document (1.945 seconds)

BMRB: 7313

2IUE

1H, 13C and 15N Backbone Resonance Assignments of the Pactolus I domain

Authors

Sen, M., Legge, G.B.

Assembly

Pactolus I-domain

Entity

1. Pactolus I-domain (polymer, Thiol state: all disulfide bound), 230 monomers, 25350.42 Da Detail

GSSHHHHHHG LVPRGSHDNS VDLYFLMGLS GSAQGHLSNV QTLGSDLLKA LNEISRSGRI GFGSIVNMTF QHILKLTADS SQFQRELRKQ LVSGKLATPK GQLDAVVQVA ICLGEIGWRN GTRFLVLVTD NDFHLAKDKT LGTRQNTSDG RCHLDDGMYR SRGEPDYQSV VQLASKLAEN NIQPIFVVPS RMVKTYEKLT TFIPKLTIGE LSDDSSNVAQ LIRNAYSKLS

Formula weight

25350.42 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS112:SG	1:CYS152:SG

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

DYANA AMBER 8.0 AND MODELLER8V2

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 77.4 %, Completeness: 37.5 %, Completeness (bb): 62.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	37.5 % (988 of 2635)	20.9 % (285 of 1365)	52.4 % (535 of 1021)	67.5 % (168 of 249)
Backbone	62.1 % (850 of 1368)	39.7 % (188 of 474)	73.9 % (495 of 670)	74.6 % (167 of 224)
Sidechain	19.9 % (294 of 1477)	10.9 % (97 of 891)	34.9 % (196 of 561)	4.0 % (1 of 25)
Aromatic	15.9 % (28 of 176)	15.9 % (14 of 88)	16.1 % (14 of 87)	0.0 % (0 of 1)
Methyl	10.8 % (29 of 268)	9.0 % (12 of 134)	12.7 % (17 of 134)

1. Pactolus I-domain

Show sample condition

Sample #1

Pressure 1 (±0) atm, Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Pactolus I-domain	[U-99% 2H; U-99% 15N; U-99% 13C]	protein	0.3 (±0.01) mM
2	dTris		salt	10 mM
3	NaN3			0.05 %
4	D20			5 %

Sample #2

Pressure 1 (±0) atm, Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Pactolus I-domain	[U-99% 15N; U-99% 13C]	protein	0.3 (±0.01) mM
6	dTris		salt	10 mM
7	NaN3			0.05 %
8	D20			5 %

Sample #3

Pressure 1 (±0) atm, Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
9	Pactolus I-domain	[U-99% 15N]	protein	0.3 (±0.01) mM
10	dTris		salt	10 mM
11	NaN3			0.05 %
12	D20			5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	Water	protons	4.7 ppm	internal	indirect	0.25144
¹H	TSP	methyl protons	0.0 ppm	external	indirect	1.0
¹⁵N	Water	protons	4.7 ppm	internal	indirect	0.10132

Referencing offset: 0.47 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	Water	protons	4.7 ppm	internal	indirect	0.25144
¹H	TSP	methyl protons	0.0 ppm	external	indirect	1.0
¹⁵N	Water	protons	4.7 ppm	internal	indirect	0.10132

Referencing offset: 0.47 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	Water	protons	4.7 ppm	internal	indirect	0.25144
¹H	TSP	methyl protons	0.0 ppm	external	indirect	1.0
¹⁵N	Water	protons	4.7 ppm	internal	indirect	0.10132

Referencing offset: 3.27 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2IUE, Strand ID: A Detail

Release date

2007-06-19

Citation

Pactolus I-domain: functional switching of the Rossmann fold
Sen, M., Legge, G.B.
Proteins (2007), 68, 626-635, PubMed 17523188 , DOI 10.1002/prot.21458 , Abstract

Related entities 1. Pactolus I-domain, : 1 : 182 entities Detail

Experiments performed 12 experiments Detail

1 HNCA

Instrument

Bruker Avance - 800 MHz Located at University of Houston-KECK NMR Center

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Pactolus I-domain	[U-99% 2H; U-99% 15N; U-99% 13C]	protein	0.3 (±0.01) mM
2	dTris		salt	10 mM
3	NaN3			0.05 %
4	D20			5 %

2 HN(CO)CA

Instrument

Bruker Avance - 800 MHz Located at University of Houston-KECK NMR Center

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Pactolus I-domain	[U-99% 2H; U-99% 15N; U-99% 13C]	protein	0.3 (±0.01) mM
2	dTris		salt	10 mM
3	NaN3			0.05 %
4	D20			5 %

3 HNCB

Instrument

Bruker Avance - 800 MHz Located at University of Houston-KECK NMR Center

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Pactolus I-domain	[U-99% 2H; U-99% 15N; U-99% 13C]	protein	0.3 (±0.01) mM
2	dTris		salt	10 mM
3	NaN3			0.05 %
4	D20			5 %

4 null

5 HN(CA)CO

Instrument

Bruker Avance - 800 MHz Located at University of Houston-KECK NMR Center

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Pactolus I-domain	[U-99% 2H; U-99% 15N; U-99% 13C]	protein	0.3 (±0.01) mM
2	dTris		salt	10 mM
3	NaN3			0.05 %
4	D20			5 %

6 HNCO

Instrument

Bruker Avance - 800 MHz Located at University of Houston-KECK NMR Center

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Pactolus I-domain	[U-99% 2H; U-99% 15N; U-99% 13C]	protein	0.3 (±0.01) mM
2	dTris		salt	10 mM
3	NaN3			0.05 %
4	D20			5 %

7 HSQC-NOESY-HSQC

Instrument

Bruker Avance - 800 MHz Located at University of Houston-KECK NMR Center

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Pactolus I-domain	[U-99% 2H; U-99% 15N; U-99% 13C]	protein	0.3 (±0.01) mM
2	dTris		salt	10 mM
3	NaN3			0.05 %
4	D20			5 %

8 CACB(CO)NH

Instrument

Bruker Avance - 800 MHz Located at University of Houston-KECK NMR Center

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Pactolus I-domain	[U-99% 2H; U-99% 15N; U-99% 13C]	protein	0.3 (±0.01) mM
2	dTris		salt	10 mM
3	NaN3			0.05 %
4	D20			5 %

9 CN-NOESY

Instrument

Bruker Avance - 800 MHz Located at University of Houston-KECK NMR Center

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Pactolus I-domain	[U-99% 2H; U-99% 15N; U-99% 13C]	protein	0.3 (±0.01) mM
2	dTris		salt	10 mM
3	NaN3			0.05 %
4	D20			5 %

10 N-TOCSY

Instrument

Bruker Avance - 800 MHz Located at University of Houston-KECK NMR Center

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
9	Pactolus I-domain	[U-99% 15N]	protein	0.3 (±0.01) mM
10	dTris		salt	10 mM
11	NaN3			0.05 %
12	D20			5 %

11 N-NOESY

Instrument

Bruker Avance - 600 MHz Located at University of Houston-KECK NMR Center

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
9	Pactolus I-domain	[U-99% 15N]	protein	0.3 (±0.01) mM
10	dTris		salt	10 mM
11	NaN3			0.05 %
12	D20			5 %

12 HSQC

Instrument

Bruker Avance - 800 MHz Located at University of Houston-KECK NMR Center

Sample

State isotropic, Pressure 1 (±0) atm, Temperature 298 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
9	Pactolus I-domain	[U-99% 15N]	protein	0.3 (±0.01) mM
10	dTris		salt	10 mM
11	NaN3			0.05 %
12	D20			5 %

nullKeywords integrin, NMR, Pactolus, Inserted domain of Pactolus, Integrin, Pactolus I-domain, Sequential backbone assignment

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.945 seconds)

BMRB: 7313

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Pactolus I-domain, : 1 : 182 entities Detail

Experiments performed 12 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample