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BMRB: 7324

2NPB

NMR solution structure of mouse SelW

Authors

Aachmann, F.L., Fomenko, D.E., Soragni, A., Gladyshev, V.N., Dikiy, A.

Assembly

SelW

Entity

1. SelW (polymer, Thiol state: all free), 96 monomers, 10689.23 Da Detail

MALAVRVVYS GACGYKPKYL QLKEKLEHEF PGCLDICGEG TPQVTGFFEV TVAGKLVHSK KRGDGYVDTE SKFRKLVTAI KAALAQCQLE HHHHHH

Formula weight

10689.23 Da

Source organism

Exptl. method

Refine. method

distance geometry, simulated annealing, energy minimisation, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.9 %, Completeness: 87.7 %, Completeness (bb): 93.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.7 % (980 of 1117)	91.2 % (527 of 578)	82.1 % (363 of 442)	92.8 % (90 of 97)
Backbone	93.9 % (535 of 570)	93.4 % (185 of 198)	94.6 % (264 of 279)	92.5 % (86 of 93)
Sidechain	83.4 % (529 of 634)	90.0 % (342 of 380)	73.2 % (183 of 250)	100.0 % (4 of 4)
Aromatic	37.5 % (39 of 104)	75.0 % (39 of 52)	0.0 % (0 of 52)
Methyl	96.2 % (102 of 106)	96.2 % (51 of 53)	96.2 % (51 of 53)

1. SelW

MALAVRVVYS GACGYKPKYL QLKEKLEHEF PGCLDICGEG TPQVTGFFEV TVAGKLVHSK KRGDGYVDTE SKFRKLVTAI KAALAQCQLE HHHHHH

Show sample condition

Sample

Temperature 298 (±0.2) K, pH 5.1 (±0.05), Details 25 mM phosphate buffer, 50 mM NaCl, 2.5 mM DTT in 90%/10% H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	SelW	[U-98% 13C; U-99% 15N]	protein	1 (±0.1) mM
2	phosphate buffer		buffer	25 mM
3	NaCl		salt	50 mM
4	DTT			2.5 mM
5	H2O			90 %
6	D2O			10 %

LACS Plot; CA

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 7 Detail

LACS Plot; CO

Referencing offset: -0.29 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2NPB, Strand ID: A Detail

Release date

2006-11-05

Citation

Solution structure of selenoprotein W and NMR analysis of its interaction with 14-3-3 proteins
Aachmann, F.L., Fomenko, D.E., Soragni, A., Gladyshev, V.N., Dikiy, A.
J. Biol. Chem. (2007), 282, 37036-37044, PubMed 17928294 , DOI 10.1074/jbc.M705410200 , Abstract

Related entities 1. SelW, : 1 : 1 : 15 entities Detail

Experiments performed 17 experiments Detail

1 1H15N_HSQC

Instrument

Bruker Avence AV - 600 MHz Cryoprobe-TXI probe

Sample

State isotropic, Temperature 298 (±0.2) K, pH 5.1 (±0.05), Details 25 mM phosphate buffer, 50 mM NaCl, 2.5 mM DTT in 90%/10% H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	SelW	[U-98% 13C; U-99% 15N]	protein	1 (±0.1) mM
2	phosphate buffer		buffer	25 mM
3	NaCl		salt	50 mM
4	DTT			2.5 mM
5	H2O			90 %
6	D2O			10 %

2 1H13C_HSQC

Instrument

Bruker Avence AV - 600 MHz Cryoprobe-TXI probe

Sample

State isotropic, Temperature 298 (±0.2) K, pH 5.1 (±0.05), Details 25 mM phosphate buffer, 50 mM NaCl, 2.5 mM DTT in 90%/10% H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	SelW	[U-98% 13C; U-99% 15N]	protein	1 (±0.1) mM
2	phosphate buffer		buffer	25 mM
3	NaCl		salt	50 mM
4	DTT			2.5 mM
5	H2O			90 %
6	D2O			10 %

3 2D_1H_NOESY

Instrument

Bruker Avence AV - 600 MHz Cryoprobe-TXI probe

Sample

State isotropic, Temperature 298 (±0.2) K, pH 5.1 (±0.05), Details 25 mM phosphate buffer, 50 mM NaCl, 2.5 mM DTT in 90%/10% H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	SelW	[U-98% 13C; U-99% 15N]	protein	1 (±0.1) mM
2	phosphate buffer		buffer	25 mM
3	NaCl		salt	50 mM
4	DTT			2.5 mM
5	H2O			90 %
6	D2O			10 %

4 2D_1H_TOCSY

Instrument

Bruker Avence AV - 600 MHz Cryoprobe-TXI probe

Sample

State isotropic, Temperature 298 (±0.2) K, pH 5.1 (±0.05), Details 25 mM phosphate buffer, 50 mM NaCl, 2.5 mM DTT in 90%/10% H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	SelW	[U-98% 13C; U-99% 15N]	protein	1 (±0.1) mM
2	phosphate buffer		buffer	25 mM
3	NaCl		salt	50 mM
4	DTT			2.5 mM
5	H2O			90 %
6	D2O			10 %

5 2D_1H_IP-COSY

Instrument

Bruker Avence AV - 600 MHz Cryoprobe-TXI probe

Sample

State isotropic, Temperature 298 (±0.2) K, pH 5.1 (±0.05), Details 25 mM phosphate buffer, 50 mM NaCl, 2.5 mM DTT in 90%/10% H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	SelW	[U-98% 13C; U-99% 15N]	protein	1 (±0.1) mM
2	phosphate buffer		buffer	25 mM
3	NaCl		salt	50 mM
4	DTT			2.5 mM
5	H2O			90 %
6	D2O			10 %

6 3D_15N-separated_NOESY

Instrument

Bruker Avence AV - 600 MHz Cryoprobe-TXI probe

Sample

State isotropic, Temperature 298 (±0.2) K, pH 5.1 (±0.05), Details 25 mM phosphate buffer, 50 mM NaCl, 2.5 mM DTT in 90%/10% H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	SelW	[U-98% 13C; U-99% 15N]	protein	1 (±0.1) mM
2	phosphate buffer		buffer	25 mM
3	NaCl		salt	50 mM
4	DTT			2.5 mM
5	H2O			90 %
6	D2O			10 %

7 3D_13C-separated_NOESY

Instrument

Bruker Avence AV - 600 MHz Cryoprobe-TXI probe

Sample

State isotropic, Temperature 298 (±0.2) K, pH 5.1 (±0.05), Details 25 mM phosphate buffer, 50 mM NaCl, 2.5 mM DTT in 90%/10% H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	SelW	[U-98% 13C; U-99% 15N]	protein	1 (±0.1) mM
2	phosphate buffer		buffer	25 mM
3	NaCl		salt	50 mM
4	DTT			2.5 mM
5	H2O			90 %
6	D2O			10 %

8 HNCA

Instrument

Bruker Avence AV - 600 MHz Cryoprobe-TXI probe

Sample

State isotropic, Temperature 298 (±0.2) K, pH 5.1 (±0.05), Details 25 mM phosphate buffer, 50 mM NaCl, 2.5 mM DTT in 90%/10% H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	SelW	[U-98% 13C; U-99% 15N]	protein	1 (±0.1) mM
2	phosphate buffer		buffer	25 mM
3	NaCl		salt	50 mM
4	DTT			2.5 mM
5	H2O			90 %
6	D2O			10 %

9 HN(CO)CA

Instrument

Bruker Avence AV - 600 MHz Cryoprobe-TXI probe

Sample

State isotropic, Temperature 298 (±0.2) K, pH 5.1 (±0.05), Details 25 mM phosphate buffer, 50 mM NaCl, 2.5 mM DTT in 90%/10% H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	SelW	[U-98% 13C; U-99% 15N]	protein	1 (±0.1) mM
2	phosphate buffer		buffer	25 mM
3	NaCl		salt	50 mM
4	DTT			2.5 mM
5	H2O			90 %
6	D2O			10 %

10 HN(CA)CO

Instrument

Bruker Avence AV - 600 MHz Cryoprobe-TXI probe

Sample

State isotropic, Temperature 298 (±0.2) K, pH 5.1 (±0.05), Details 25 mM phosphate buffer, 50 mM NaCl, 2.5 mM DTT in 90%/10% H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	SelW	[U-98% 13C; U-99% 15N]	protein	1 (±0.1) mM
2	phosphate buffer		buffer	25 mM
3	NaCl		salt	50 mM
4	DTT			2.5 mM
5	H2O			90 %
6	D2O			10 %

11 HNCO

Instrument

Bruker Avence AV - 600 MHz Cryoprobe-TXI probe

Sample

State isotropic, Temperature 298 (±0.2) K, pH 5.1 (±0.05), Details 25 mM phosphate buffer, 50 mM NaCl, 2.5 mM DTT in 90%/10% H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	SelW	[U-98% 13C; U-99% 15N]	protein	1 (±0.1) mM
2	phosphate buffer		buffer	25 mM
3	NaCl		salt	50 mM
4	DTT			2.5 mM
5	H2O			90 %
6	D2O			10 %

12 CACBNH

Instrument

Bruker Avence AV - 600 MHz Cryoprobe-TXI probe

Sample

State isotropic, Temperature 298 (±0.2) K, pH 5.1 (±0.05), Details 25 mM phosphate buffer, 50 mM NaCl, 2.5 mM DTT in 90%/10% H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	SelW	[U-98% 13C; U-99% 15N]	protein	1 (±0.1) mM
2	phosphate buffer		buffer	25 mM
3	NaCl		salt	50 mM
4	DTT			2.5 mM
5	H2O			90 %
6	D2O			10 %

13 CACB(CO)NH

Instrument

Bruker Avence AV - 600 MHz Cryoprobe-TXI probe

Sample

State isotropic, Temperature 298 (±0.2) K, pH 5.1 (±0.05), Details 25 mM phosphate buffer, 50 mM NaCl, 2.5 mM DTT in 90%/10% H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	SelW	[U-98% 13C; U-99% 15N]	protein	1 (±0.1) mM
2	phosphate buffer		buffer	25 mM
3	NaCl		salt	50 mM
4	DTT			2.5 mM
5	H2O			90 %
6	D2O			10 %

14 HAHBNH

Instrument

Bruker Avence AV - 600 MHz Cryoprobe-TXI probe

Sample

State isotropic, Temperature 298 (±0.2) K, pH 5.1 (±0.05), Details 25 mM phosphate buffer, 50 mM NaCl, 2.5 mM DTT in 90%/10% H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	SelW	[U-98% 13C; U-99% 15N]	protein	1 (±0.1) mM
2	phosphate buffer		buffer	25 mM
3	NaCl		salt	50 mM
4	DTT			2.5 mM
5	H2O			90 %
6	D2O			10 %

15 HAHB(CO)NH

Instrument

Bruker Avence AV - 600 MHz Cryoprobe-TXI probe

Sample

State isotropic, Temperature 298 (±0.2) K, pH 5.1 (±0.05), Details 25 mM phosphate buffer, 50 mM NaCl, 2.5 mM DTT in 90%/10% H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	SelW	[U-98% 13C; U-99% 15N]	protein	1 (±0.1) mM
2	phosphate buffer		buffer	25 mM
3	NaCl		salt	50 mM
4	DTT			2.5 mM
5	H2O			90 %
6	D2O			10 %

16 HCCH-TOCSY

Instrument

Bruker Avence AV - 600 MHz Cryoprobe-TXI probe

Sample

State isotropic, Temperature 298 (±0.2) K, pH 5.1 (±0.05), Details 25 mM phosphate buffer, 50 mM NaCl, 2.5 mM DTT in 90%/10% H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	SelW	[U-98% 13C; U-99% 15N]	protein	1 (±0.1) mM
2	phosphate buffer		buffer	25 mM
3	NaCl		salt	50 mM
4	DTT			2.5 mM
5	H2O			90 %
6	D2O			10 %

17 HCCH-COSY

Instrument

Bruker Avence AV - 600 MHz Cryoprobe-TXI probe

Sample

State isotropic, Temperature 298 (±0.2) K, pH 5.1 (±0.05), Details 25 mM phosphate buffer, 50 mM NaCl, 2.5 mM DTT in 90%/10% H2O/D2O

#	Name	Isotope labeling	Type	Concentration
1	SelW	[U-98% 13C; U-99% 15N]	protein	1 (±0.1) mM
2	phosphate buffer		buffer	25 mM
3	NaCl		salt	50 mM
4	DTT			2.5 mM
5	H2O			90 %
6	D2O			10 %

nullKeywords NMR, selenoprotein, SelW