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BMRB: 7339


7339
2I9Y
Solution structure of Arabidopsis thaliana protein At1g70830, a member of the major latex protein family
Authors
Volkman, B.F., de la Cruz, N.B., Lytle, B.L., Peterson, F.C.
Assembly
MLP-like protein 28
Entity
1. MLP-like protein 28 (polymer, Thiol state: all free), 166 monomers, 18650.71 Da Detail

GHHHHHHLET EASSLVGKLE TDVEIKASAD KFHHMFAGKP HHVSKASPGN IQGCDLHEGD WGTVGSIVFW NYVHDGEAKV AKERIEAVEP DKNLITFRVI EGDLMKEYKS FLLTIQVTPK PGGPGSIVHW HLEYEKISEE VAHPETLLQF CVEVSKEIDE HLLAEE


Formula weight
18650.71 Da
Source organism
Arabidopsis thaliana
Exptl. method
solution NMR
Refine. method
AUTOMATED METHODS WERE USED FOR BACKBONE CHEMICAL SHIFT ASSIGNMENT AND ITERATIVE NOE REFINEMENT.
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.6 %, Completeness: 88.4 %, Completeness (bb): 92.8 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All88.4 % (1700 of 1922)87.9 % (871 of 991)87.7 % (669 of 763)95.2 % (160 of 168)
Backbone92.8 % (911 of 982)91.1 % (308 of 338)93.2 % (452 of 485)95.0 % (151 of 159)
Sidechain85.0 % (929 of 1093)85.9 % (561 of 653)83.3 % (359 of 431)100.0 % (9 of 9)
Aromatic54.9 % (101 of 184)62.0 % (57 of 92)46.1 % (41 of 89)100.0 % (3 of 3)
Methyl94.0 % (171 of 182)94.5 % (86 of 91)93.4 % (85 of 91)

1. MLP-like protein 28

GHHHHHHLET EASSLVGKLE TDVEIKASAD KFHHMFAGKP HHVSKASPGN IQGCDLHEGD WGTVGSIVFW NYVHDGEAKV AKERIEAVEP DKNLITFRVI EGDLMKEYKS FLLTIQVTPK PGGPGSIVHW HLEYEKISEE VAHPETLLQF CVEVSKEIDE HLLAEE

Show sample condition
Sample

Solvent system 95% H2O, 5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.7 mM At1g70830 U-15N/13C protein, 10 mM deuterated bis-tris, 5 mM Dithiothreitol, 95% H2O, 5% D2O


#NameIsotope labelingTypeConcentration
1MLP-like protein 28[U-13C; U-15N]0.7 mM
2bis-tris[U-2H]10 mM
3Dithiothreitolnone5 mM
4H2Onone95 %
5D2Onone5 %

LACS Plot; CA
Referencing offset: -0.58 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.58 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.14 ppm, Outliers: 4 Detail
LACS Plot; CO
Referencing offset: -0.06 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2I9Y, Strand ID: A Detail

Release date
2010-11-15
Citation
Structures of two Arabidopsis thaliana major latex proteins represent novel helix-grip folds
Lytle, B.L., Song, J., de la Cruz, N.B., Peterson, F.C., Johnson, K.A., Bingman, C.A., Phillips, G.N., Volkman, B.F.
Proteins (2009), 76, 237-243, PubMed 19326460 , DOI 10.1002/prot.22396 ,
Related entities 1. MLP-like protein 28, : 1 : 85 entities Detail
More details for 86 related entities
Interaction partners 1. MLP-like protein 28, : 1 interactors Detail
More details for 1 interactors identified by 1 citations
Experiments performed 3 experiments Detail
nullKeywords At1g70830, Bet v1-like, Center for Eukaryotic Structural Genomics, CESG, Protein Structure Initiative, PSI, Structural Genomics