BMRB: 7342

SOLUTION STRUCTURE OF THE ZN COMPLEX OF HIV-2 NCP(23-49) PEPTIDE, ENCOMPASSING PROTEIN CCHC-LINKER, DISTAL CCHC ZN-BINDING MOTIF AND C-TERMINAL TAIL

Authors

Amodeo, P., Castiglione Morelli, M.A., Ostuni, A., Cristinziano, P., Bavoso, A.

Assembly

NUCLEOCAPSID PROTEIN

Entity

1. NUCLEOCAPSID PROTEIN (polymer, Thiol state: all other bound), 27 monomers, 3012.521 Da Detail

RAPRRQGCWK CGKTGHVMAK CPERQAG

2. ZN (non-polymer), 65.409 Da

Total weight

3077.93 Da

Max. entity weight

3012.521 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS8:SG	2:ZN1:ZN
2	na	sing	1:CYS11:SG	2:ZN1:ZN
3	na	sing	1:HIS16:NE2	2:ZN1:ZN
4	na	sing	1:CYS21:SG	2:ZN1:ZN

Source organism

Human immunodeficiency virus 2

Exptl. method

solution NMR

Refine. method

SIMULATED ANNEALING-ENERGY MINIMIZATION

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.8 %, Completeness (bb): 98.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	95.8 % (160 of 167)	95.8 % (160 of 167)
Backbone	98.2 % (55 of 56)	98.2 % (55 of 56)
Sidechain	94.6 % (105 of 111)	94.6 % (105 of 111)
Aromatic	100.0 % (8 of 8)	100.0 % (8 of 8)
Methyl	100.0 % (6 of 6)	100.0 % (6 of 6)

1. NUCLEOCAPSID PROTEIN

RAPRRQGCWK CGKTGHVMAK CPERQAG

Show sample condition

Sample

Pressure 1.0 atm, Temperature 298.0 K, pH 7.0, Details 80% WATER/20% D2O

#	Name	Isotope labeling	Type	Concentration
1	NCp8	natural abundance		2.6 mM
2	H2O	natural abundance		80 %
3	D2O	natural abundance		20 %
4	TCEP.HCl	natural abundance		11.5 mM
5	zinc chloride	natural abundance		3.1 mM

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Protein Blocks Logo

Calculated from 30 models in PDB: 2IWJ, Strand ID: A Detail

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Release date

2011-05-25

Citation

Structural Features of the C-Terminal Zinc Finger Domain of the HIV-2 Nc Protein (Residues 23-49)
Amodeo, P., Castiglione Morelli, M.A., Ostuni, A., Cristinziano, P., Bavoso, A.

Related entities 1. NUCLEOCAPSID PROTEIN, : 1 : 6 : 2 : 4 : 147 entities Detail

Interaction partners 1. NUCLEOCAPSID PROTEIN, : 1 interactors Detail

Experiments performed 3 experiments Detail