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Found 1 Document (1.632 seconds)

BMRB: 7349

2C0S

NMR SOLUTION STRUCTURE OF A PROTEIN ASPARTIC ACID PHOSPHATE PHOSPHATASE FROM BACILLUS ANTHRACIS

Authors

Grenha, R., Rzechorzek, N., Brannigan, J.A., Ab, E., Folkers, G.E., De Jong, R.N., Diercks, T., Wilkinson, A.J., Kaptein, R., Wilson, K.S.

Assembly

CONSERVED DOMAIN PROTEIN

Entity

1. CONSERVED DOMAIN PROTEIN (polymer, Thiol state: not present), 57 monomers, 6878.087 Da Detail

MNVTKLNDRI EAKKKELIYL VEKYGFTHHK VISFSQELDR LLNLLIELKT KKKRYSL

Formula weight

6878.087 Da

Source organism

Bacillus anthracis

Exptl. method

Refine. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.5 %, Completeness (bb): 95.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.5 % (676 of 747)	87.6 % (346 of 395)	93.1 % (271 of 291)	96.7 % (59 of 61)
Backbone	95.6 % (327 of 342)	93.0 % (107 of 115)	96.5 % (164 of 170)	98.2 % (56 of 57)
Sidechain	87.4 % (403 of 461)	85.4 % (239 of 280)	91.0 % (161 of 177)	75.0 % (3 of 4)
Aromatic	84.6 % (44 of 52)	84.6 % (22 of 26)	84.6 % (22 of 26)
Methyl	85.5 % (65 of 76)	84.2 % (32 of 38)	86.8 % (33 of 38)

1. CONSERVED DOMAIN PROTEIN

MNVTKLNDRI EAKKKELIYL VEKYGFTHHK VISFSQELDR LLNLLIELKT KKKRYSL

Show sample condition

Sample

Pressure 1.0 atm, Temperature 278.0 K, pH 6.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

Protein Blocks Logo

Calculated from 25 models in PDB: 2C0S, Strand ID: A Detail

Release date

2008-08-13

Citation

Structural characterization of Spo0E-like protein-aspartic acid phosphatases that regulate sporulation in bacilli
Grenha, R., Rzechorzek, N.J., Brannigan, J.A., de Jong, R.N., AB, E., Diercks, T., Truffault, V., Ladds, J.C., Fogg, M.J., Bongiorni, C., Perego, M., Kaptein, R., Wilson, K.S., Folkers, G.E., Wilkinson, A.J.
J. Biol. Chem. (2006), 281, 37993-38003, PubMed 17001075 , DOI 10.1074/jbc.M607617200 , Abstract

Related entities 1. CONSERVED DOMAIN PROTEIN, : 1 : 9 entities Detail

Experiments performed 14 experiments Detail

1 HNCO

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 278.0 K, pH 6.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

2 HNCACO

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 278.0 K, pH 6.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

3 HNCACB

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 278.0 K, pH 6.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

4 CBCACONH

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 278.0 K, pH 6.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

5 HNCA

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 278.0 K, pH 6.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

6 HBHACONH

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 278.0 K, pH 6.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

7 HNCAHA

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 278.0 K, pH 6.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

8 CCH-COSY

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 278.0 K, pH 6.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

9 HCCH-TOCSY

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 278.0 K, pH 6.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

10 CCCONH

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 278.0 K, pH 6.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

11 HNH-NOESY

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 278.0 K, pH 6.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

12 HCH-NOESY

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 278.0 K, pH 6.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

13 CNH-NOESY

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 278.0 K, pH 6.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

14 HH-NOESY

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 278.0 K, pH 6.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

nullKeywords ANTHRACIS, ANTITHETICAL, BACILLUS, NEGATIVE, PHOSPHATASE, PHOSPHORYLATION, REGULATOR, SPINE, SPORULATION