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Found 1 Document (1.732 seconds)

BMRB: 7350

2BZB

NMR SOLUTION STRUCTURE OF A PROTEIN ASPARTIC ACID PHOSPHATE PHOSPHATASE FROM BACILLUS ANTHRACIS

Authors

Grenha, R., Rzechorzek, N., Brannigan, J.A., Ab, E., Folkers, G., Dejong, R., Diercks, T., Wilkinson, A.J., Kaptein, R., Wilson, K.S.

Assembly

CONSERVED DOMAIN PROTEIN

Entity

1. CONSERVED DOMAIN PROTEIN (polymer, Thiol state: not present), 54 monomers, 6428.592 Da Detail

MEMGQLKNKI ENKKKELIQL VARHGLDHDK VLLFSRDLDK LINKFMNVKD KVHK

Formula weight

6428.592 Da

Source organism

Bacillus anthracis

Exptl. method

Refine. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.3 %, Completeness (bb): 96.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.3 % (672 of 698)	96.2 % (359 of 373)	96.2 % (255 of 265)	96.7 % (58 of 60)
Backbone	96.0 % (311 of 324)	94.5 % (104 of 110)	96.9 % (155 of 160)	96.3 % (52 of 54)
Sidechain	96.9 % (413 of 426)	97.0 % (255 of 263)	96.8 % (152 of 157)	100.0 % (6 of 6)
Aromatic	68.8 % (22 of 32)	68.8 % (11 of 16)	68.8 % (11 of 16)
Methyl	100.0 % (62 of 62)	100.0 % (31 of 31)	100.0 % (31 of 31)

1. CONSERVED DOMAIN PROTEIN

MEMGQLKNKI ENKKKELIQL VARHGLDHDK VLLFSRDLDK LINKFMNVKD KVHK

Show sample condition

Sample

Pressure 1.0 atm, Temperature 298.0 K, pH 7.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

LACS Plot; CA

Referencing offset: 2.43 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: 2.43 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: 2.65 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 32 models in PDB: 2BZB, Strand ID: A, B Detail

Release date

2008-08-13

Citation

Structural characterization of Spo0E-like protein-aspartic acid phosphatases that regulate sporulation in bacilli
Grenha, R., Rzechorzek, N.J., Brannigan, J.A., de Jong, R.N., AB, E., Diercks, T., Truffault, V., Ladds, J.C., Fogg, M.J., Bongiorni, C., Perego, M., Kaptein, R., Wilson, K.S., Folkers, G.E., Wilkinson, A.J.
J. Biol. Chem. (2006), 281, 37993-38003, PubMed 17001075 , DOI 10.1074/jbc.M607617200 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 7349 released on 2008-08-13
Title NMR SOLUTION STRUCTURE OF A PROTEIN ASPARTIC ACID PHOSPHATE PHOSPHATASE FROM BACILLUS ANTHRACIS

Related entities 1. CONSERVED DOMAIN PROTEIN, : 1 : 6 entities Detail

Experiments performed 13 experiments Detail

1 HNCO

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 298.0 K, pH 7.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

2 HNCACO

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 298.0 K, pH 7.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

3 HNCACB

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 298.0 K, pH 7.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

4 CBCACONH

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 298.0 K, pH 7.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

5 HNCA

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 298.0 K, pH 7.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

6 HBHACONH

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 298.0 K, pH 7.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

7 HNCAHA

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 298.0 K, pH 7.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

8 CCH-COSY

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 298.0 K, pH 7.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

9 HCCH-TOCSY

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 298.0 K, pH 7.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

10 HNH-NOESY

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 298.0 K, pH 7.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

11 HCH-NOESY

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 298.0 K, pH 7.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

12 CNH- NOESY

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 298.0 K, pH 7.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

13 HH-NOESY

Instrument

Bruker OTHER - 700 MHz

Sample

State isotropic, Pressure 1.0 atm, Temperature 298.0 K, pH 7.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CONSERVED_DOMAIN_PROTEIN	natural abundance		0.0 ~ 0.0 mM
2	D2O			10 %
3	H2O	natural abundance		90 %

nullKeywords ANTHRACIS, ANTITHETICAL, BACILLUS, NEGATIVE, PHOSPHATASE, PHOSPHORYLATION, REGULATOR, SPINE, SPORULATION