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Found 1 Document (1.887 seconds)

BMRB: 7351

2BZE

NMR STRUCTURE OF HUMAN RTF1 PLUS3 DOMAIN.

Authors

Truffault, V., Diercks, T., Ab, E., De Jong, R.N., Daniels, M., Kaptein, R., Folkers, G.E.

Assembly

KIAA0252 PROTEIN

Entity

1. KIAA0252 PROTEIN (polymer, Thiol state: all free), 153 monomers, 17534.87 Da Detail

MGSSHHHHHH SSGLVPRGSH MVSLPEELNR VRLSRHKLER WCHMPFFAKT VTGCFVRIGI GNHNSKPVYR VAEITGVVET AKVYQLGGTR TNKGLQLRHG NDQRVFRLEF VSNQEFTESE FMKWKEAMFS AGMQLPTLDE INKKELSIKE ALN

Formula weight

17534.87 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.7 %, Completeness: 92.0 %, Completeness (bb): 93.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.0 % (1674 of 1820)	93.2 % (887 of 952)	89.9 % (634 of 705)	93.9 % (153 of 163)
Backbone	93.5 % (849 of 908)	93.9 % (294 of 313)	93.3 % (417 of 447)	93.2 % (138 of 148)
Sidechain	91.0 % (958 of 1053)	92.8 % (593 of 639)	87.7 % (350 of 399)	100.0 % (15 of 15)
Aromatic	63.4 % (104 of 164)	70.7 % (58 of 82)	55.0 % (44 of 80)	100.0 % (2 of 2)
Methyl	100.0 % (148 of 148)	100.0 % (74 of 74)	100.0 % (74 of 74)

1. KIAA0252 PROTEIN

MGSSHHHHHH SSGLVPRGSH MVSLPEELNR VRLSRHKLER WCHMPFFAKT VTGCFVRIGI GNHNSKPVYR VAEITGVVET AKVYQLGGTR TNKGLQLRHG NDQRVFRLEF VSNQEFTESE FMKWKEAMFS AGMQLPTLDE INKKELSIKE ALN

Show sample condition

Sample

Pressure 1.0 atm, Temperature 298.0 K, pH 6.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Concentration
1	KIAA0252_PROTEIN	[U-13C; U-15N]	0.0 ~ 0.0 mM
2	NaCl		150 mM
3	Na_2 HPO_4		50 mM
4	NaH_2 PO_4		50 mM
5	D2O		10 %
6	H2O	natural abundance	90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.57 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.57 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.54 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 25 models in PDB: 2BZE, Strand ID: A Detail

Release date

2007-10-22

Citation

Structure and DNA binding of the human Rtf1 Plus3 domain
De Jong, R.N., Truffault, V., Diercks, T., Ab, E., Daniels, M.A., Kaptein, R., Folkers, G.E.
Structure (2008), 16, 149-159, PubMed 18184592 , DOI 10.1016/j.str.2007.10.018 , Abstract

Related entities 1. KIAA0252 PROTEIN, : 1 : 2 : 34 entities Detail

Interaction partners 1. KIAA0252 PROTEIN, : 13 interactors Detail

More details for 13 interactors identified by 7 citations

Experiments performed 9 experiments Detail

1 HH-NOESY