BMRB: 7352

SOLUTION STRUCTURE OF THE ZN COMPLEX OF EIAV NCP11(22-58) PEPTIDE, INCLUDING TWO CCHC ZN-BINDING MOTIFS.

Authors

Amodeo, P., Castiglione-Morelli, M.A., Ostuni, A., Bavoso, A.

Assembly

GAG POLYPROTEIN

Entity

1. GAG POLYPROTEIN - NUCLEOCAPSID PROTEIN P11 (polymer, Thiol state: all other bound), 37 monomers, 4143.798 Da Detail

QTCYNCGKPG HLSSQCRAPK VCFKCKQPGH FSKQCRS

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

4274.6157 Da

Max. entity weight

4143.798 Da

Source organism

Human immunodeficiency virus 2

Exptl. method

solution NMR

Refine. method

SIMULATED ANNEALING-ENERGY MINIMIZATION

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.4 %, Completeness (bb): 98.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	97.4 % (227 of 233)	97.4 % (227 of 233)
Backbone	98.6 % (73 of 74)	98.6 % (73 of 74)
Sidechain	96.9 % (154 of 159)	96.9 % (154 of 159)
Aromatic	100.0 % (18 of 18)	100.0 % (18 of 18)
Methyl	100.0 % (6 of 6)	100.0 % (6 of 6)

1. GAG POLYPROTEIN - NUCLEOCAPSID PROTEIN P11

QTCYNCGKPG HLSSQCRAPK VCFKCKQPGH FSKQCRS

Show sample condition

Sample

Pressure 1.0 atm, Temperature 293.0 K, pH 5.8, Details 10% WATER/90% D2O

#	Name	Isotope labeling	Type	Concentration
1	NCp8	natural abundance		2.5 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	TCEP.HCl	natural abundance		37.5 mM
5	zinc chloride	natural abundance		7.5 mM

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Protein Blocks Logo

Calculated from 30 models in PDB: 2BL6, Strand ID: A Detail

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Release date

2007-06-05

Citation

Structural features in EIAV NCp11: a lentivirus nucleocapsid protein with a short linker
Amodeo, P., Castiglione-Morelli, M.A., Ostuni, A., Battistuzzi, G., Bavoso, A.
Biochemistry (2006), 45, 5517-5526, PubMed 16634633 , DOI 10.1021/bi0524924 , Abstract

Related entities 1. GAG POLYPROTEIN - NUCLEOCAPSID PROTEIN P11, : 1 : 1 : 360 entities Detail

Interaction partners 1. GAG POLYPROTEIN - NUCLEOCAPSID PROTEIN P11, : 1 interactors Detail

Experiments performed 2 experiments Detail