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BMRB: 7354

2BJC

NMR STRUCTURE OF A PROTEIN-DNA COMPLEX OF AN ALTERED SPECIFICITY MUTANT OF THE LAC REPRESSOR THAT MIMICS THE GAL REPRESSOR

Authors

Salinas, R.K., Folkers, G.E., Bonvin, A.M.J.J., Das, D., Boelens, R., Kaptein, R.

Assembly

LAC REPRESSOR

Entity

1. LAC-GAL OPERATOR (polymer), 22 monomers, 6813.361 × 2 Da Detail

GAATTGTAAG CGCTTACAAT TC

2. LAC HEADPIECE 1-62 (polymer), 62 monomers, 6693.594 × 2 Da Detail

MKPVTLYDVA EYAGVSVATV SRVVNQASHV SAKTREKVEA AMAELNYIPN RCAQQLAGKQ SL

Total weight

27013.91 Da

Max. entity weight

6813.361 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	2:CYS52:SG	2:CYS52:SG

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

HADDOCK

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 85.6 %, Completeness (bb): 85.2 % Detail

Polymer type: polydeoxyribonucleotide polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.6 % (773 of 903)	81.0 % (460 of 568)	92.2 % (247 of 268)	98.5 % (66 of 67)
Backbone	85.2 % (445 of 522)	75.9 % (211 of 278)	95.1 % (175 of 184)	98.3 % (59 of 60)
Sidechain	87.5 % (386 of 441)	85.9 % (249 of 290)	90.3 % (130 of 144)	100.0 % (7 of 7)
Aromatic	66.7 % (48 of 72)	69.0 % (40 of 58)	57.1 % (8 of 14)
Methyl	100.0 % (89 of 89)	100.0 % (48 of 48)	100.0 % (41 of 41)

1. LAC-GAL OPERATOR

GAATTGTAAG CGCTTACAAT TC

2. LAC HEADPIECE 1-62

MKPVTLYDVA EYAGVSVATV SRVVNQASHV SAKTREKVEA AMAELNYIPN RCAQQLAGKQ SL

Show sample condition

Sample

Pressure 1.0 atm, Temperature 315.0 K, pH 6.0, Details 95% WATER/5% D2O

#	Name	Isotope labeling	Concentration
1	LAC_REPRESSOR	natural abundance	0.0 ~ 0.0 mM
2	D2O		5 %
3	H2O	natural abundance	95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	internal	null
¹H	na	na	internal	null
¹⁵N	na	na	internal	null

Referencing offset: -0.46 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	internal	null
¹H	na	na	internal	null
¹⁵N	na	na	internal	null

Referencing offset: -0.46 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	internal	null
¹H	na	na	internal	null
¹⁵N	na	na	internal	null

Referencing offset: 0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type
¹³C	na	na	internal	null
¹H	na	na	internal	null
¹⁵N	na	na	internal	null

Referencing offset: -0.65 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 16 models in PDB: 2BJC, Strand ID: A, B Detail

Release date

2010-02-24

Citation 1

Altered specificity in DNA binding by the lac repressor: a mutant lac headpiece that mimics the gal repressor
Kopke Salinas, R., Folkers, G.E., Bonvin, A.M.J.J., Das, D., Boelens, R., Kaptein, R.
Chembiochem. (2005), 6, 1628-1637, PubMed 16094693 , DOI 10.1002/cbic.200500049 , Abstract

Show more 2 citaions

Citation 2

Plasticity in Protein-DNA Recognition: Lac Repressor Interacts with its Natural Operator O1 Through Alternative Conformations of its DNA Binding Domain
Kalodimos, C.G., Bonvin, A.M.J.J., Salinas, R.K., Wechselberger, R., Boelens, R., Kaptein, R.
Embo J. (2002), 21, 2866, PubMed , DOI:

Citation 3

The interaction of the recognition helix of lac repressor with lac operator
Lehming, N., Sartorius, J., Niemoler, M., Genenger, G., Wilcken-Bergmann, B., Muller-Hill, B.
EMBO J. (1987), 6, 3145-3153, PubMed 2826131 , DOI: , Abstract

Hide non-primary 2 citaions

Related entities 2. LAC HEADPIECE 1-62, : 1 : 18 : 176 entities Detail

Interaction partners 2. LAC HEADPIECE 1-62, : 6 interactors Detail

More details for 6 interactors identified by 2 citations

Experiments performed 12 experiments Detail

1 3D 15N NOESY-HSQC