1H, 15N and 13C chemical shift assignments for reduced and oxidised forms of the DsbA oxidoreductase from Vibrio cholerae
AQFKEGEHYQ VLKTPASSSP VVSEFFSFYC PHCNTFEPII AQLKQQLPEG AKFQKNHVSF MGGNMGQAMS KAYATMIALE VEDKMVPVMF NRIHTLRKPP KDEQELRQIF LDEGIDAAKF DAAYNGFAVD SMVRRFDKQF QDSGLTGVPA VVVNNRYLVQ GQSAKSLDEY FDLVNYLLTL K
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 80.3 % (1735 of 2161) | 86.8 % (977 of 1126) | 68.8 % (579 of 842) | 92.7 % (179 of 193) |
Backbone | 84.8 % (906 of 1068) | 96.1 % (349 of 363) | 72.4 % (386 of 533) | 99.4 % (171 of 172) |
Sidechain | 78.9 % (997 of 1264) | 82.3 % (628 of 763) | 75.2 % (361 of 480) | 38.1 % (8 of 21) |
Aromatic | 24.1 % (51 of 212) | 39.6 % (42 of 106) | 8.5 % (9 of 106) | |
Methyl | 99.5 % (181 of 182) | 98.9 % (90 of 91) | 100.0 % (91 of 91) |
1. DsbA protein disulfide isomerase
AQFKEGEHYQ VLKTPASSSP VVSEFFSFYC PHCNTFEPII AQLKQQLPEG AKFQKNHVSF MGGNMGQAMS KAYATMIALE VEDKMVPVMF NRIHTLRKPP KDEQELRQIF LDEGIDAAKF DAAYNGFAVD SMVRRFDKQF QDSGLTGVPA VVVNNRYLVQ GQSAKSLDEY FDLVNYLLTL KTemperature 320 (±0.2) K, pH 6.8, Details 10mM HEPES 50mM NaCl pH 6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DsbA - Bacterial oxidoreductase | [U-95% 13C; U-90% 15N] | protein | |
2 | HEPES | 10 mM | ||
3 | NaCl | 50 mM |