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BMRB: 7362


7362
2NWT
NMR Structure of Protein UPF0165 protein AF_2212 from Archaeoglobus Fulgidus; Northeast Structural Genomics Consortium Target GR83
Authors
Singarapu, K.K., Sukumaran, D.K., Parish, D., Atreya, H.S., Liu, G., Eletsky, A., Chen, C.X., Jiang, M., Cunningham, K., Xiao, R., Swapna, G.V.T., Acton, T.B., Rost, B., Montelione, G.T., Szyperski, T.
Assembly
UPF0165
Entity
1. UPF0165 (polymer, Thiol state: not present), 69 monomers, 8046.234 × 2 Da Detail

MPKIIEAVYE NGVFKPLQKV DLKEGERVKI KLELKVEPID LGEPVSVEEI KKIRDGTWMS SLEHHHHHH


Total weight
16092.468 Da
Max. entity weight
8046.234 Da
Source organism
Archaeoglobus fulgidus
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 89.9 %, Completeness: 78.3 %, Completeness (bb): 72.9 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All78.3 % (666 of 851)84.9 % (381 of 449)67.7 % (226 of 334)86.8 % (59 of 68)
Backbone72.9 % (296 of 406)86.2 % (119 of 138)59.6 % (121 of 203)86.2 % (56 of 65)
Sidechain83.7 % (427 of 510)84.2 % (262 of 311)82.7 % (162 of 196)100.0 % (3 of 3)
Aromatic51.9 % (28 of 54)59.3 % (16 of 27)42.3 % (11 of 26)100.0 % (1 of 1)
Methyl92.5 % (74 of 80)90.0 % (36 of 40)95.0 % (38 of 40)

1. UPF0165 protein AF 2212

MPKIIEAVYE NGVFKPLQKV DLKEGERVKI KLELKVEPID LGEPVSVEEI KKIRDGTWMS SLEHHHHHH

Show sample condition
Sample

Solvent system 5% D2O, 95% H2O, Pressure 1 atm, Temperature 298 K, pH 5.5, Details 1.2 mM protein Y2212_ARCFU, U-15N,13C, 5mM CaCl, 100mM NaCl, 20mM NH4OAc, 10mM DTT, 0.02%NaN3, 5% D2O, 95% H2O


#NameIsotope labelingTypeConcentration
1UPF0165[U-13C; U-15N]1.2 mM
2NaCl5 mM
3NH4OAc20 mM
4DTT10 mM
5NaN30.02 %
6D2O5 %
7H2Onatural abundance95 %

LACS Plot; CA
Referencing offset: -0.14 ppm, Outliers: 4 Detail
LACS Plot; CB
Referencing offset: -0.14 ppm, Outliers: 4 Detail
LACS Plot; HA
Referencing offset: 0.06 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2NWT, Strand ID: A, B Detail

Release date
2010-03-03
Citation
NMR Structure of Protein Y2212_ARCFU from Archaeoglobus Fulgidus; Northeast Structural Genomics Consortium Target GR83
Singarapu, K.K., Sukumaran, D.K., Parish, D., Atreya, H.S., Liu, G., Eletsky, A., Chen, C.X., Jiang, M., Cunningham, K., Ma, L.C., Xiao, R., Liu, J., Baran, M., Swapna, G.V.T., Acton, T.B., Rost, B., Montelione, G.T., Szyperski, T.
Related entities 1. UPF0165, : 1 : 1 : 2 entities Detail
More details for 4 related entities
Experiments performed 5 experiments Detail
nullKeywords GFT NMR, Homo Dimer Protein, NESG, Northeast Structural Genomics Consortium, Protein Structure Initiative, PSI-2, Structural Genomics